Protein cross-linking inhibitor

ABSTRACT

The present invention provides a protein cross-linking inhibitor containing a compound represented by any of the following formulas (1)-(13), or a pharmaceutically acceptable salt thereof:
 
R 3 —[—X—B(ZR 1 )—Y—B(ZR 2 )—W—] n —R 4 ,  (1)
 
R 3 —[—X—B(ZR 1 )—Y—] n —R 4 ,  (2)
 
R 3 —[—B(ZR 1 )—Y—B(ZR 2 )—W—] n —R 4 ,  (3)
 
R 3 —[—X—B(ZR 1 )—] n —R 4 ,  (4)
 
R 3 —[—B(ZR 2 )—W—] n —R 4 ,  (5)
 
R 3 —X—B(ZR 1 )-T[B(ZR 2 )—W—R 4 ] 2 ,  (6)
 
R 3 —B(OH) 2 ,  (7)
 
R 3 —B(ZR 1 )—X—B(ZR 2 )—R 4 ,  (8)
 
R 3 —B(R 1 )—O—B(R 2 )—R 4 ,  (9)
 
R 3 —[—X—B(ZR 1 )—Y—B(ZR 2 )—] n —R 4 ,  (10)
 
R 3 —[—X—B(ZR 1 )—Y—B(ZR 2 )—W-Q-] n —R 4 ,  (11)
 
R 3 —[—P—X—B(ZR 1 )—Y—B(ZR 2 )—W—] n —R 4 ,  (12)
 
[R 3 —X—B(ZR 1 )—Y] 2 B(ZR 2 ),  (13)
 
wherein each symbol is as defined in the DESCRIPTION.

TECHNICAL FIELD

The present invention relates to a protein cross-linking inhibitorcomprising a boron compound. Furthermore, the present invention relatesto a novel boron compound useful for use thereof.

BACKGROUND ART

Calcium ion is essential for the body, and the concentration ofintracellular Ca²⁺ constituting the body is as extremely low as 10⁻⁷M,which is 1 to 10,000 relative to the extracellular concentration. Whenthe cell is stimulated, intracellular Ca²⁺ increases to generate Ca²⁺wave that produces slow intracellular Ca²⁺ oscillation, and inducesphysiological function.

SOCE (store-operated calcium entry) is also called capacitive calciumentry, which is a mechanism that causes extracellular influx of Ca²⁺ forreplenishment of depleted intracellular Ca²⁺ stores, and important forlong-term sustainability of intracellular Ca²⁺ signals.

SOCE is measured as Icrac (calcium release-activated calcium-selectivecurrent). It has been clarified that SOCE and Icrac channel aredefective in the T cells of patients with severe combinedimmunodeficiency (SCID). Furthermore, it has also been clarified that aprotein called STIM (stromal interaction molecule) senses depletion ofCa²⁺ in the endoplasmic reticulum, passes the information to thecellular membrane, and activates CRACM (calcium release-activatedcalcium modulator) (Orai) located in the cellular membrane and formsIcrac channel pore.

Extracellular stimulus is recognized by a receptor on the cellularmembrane, the information thereof activates PLC (phospholipase C) via Gprotein and hydrolyzes PIP2 (phosphatidylinositol bisphosphate), whichis an inositolphospholipid in the cellular membrane, and producesdiacylglycerol and IP3 (inositol trisphosphate). Diacylglycerolactivates protein kinase C and phosphorylates protein, causing variousphysiological phenomena. IP3 acts on IP3 receptor to cause release ofCa²⁺. The present inventors have found an IP3 receptor molecule inmutant mouse, and successfully determined all base sequences of themembrane protein (non-patent document 1). In addition, they haveclarified that the IP3 receptor localizes in the endoplasmic reticulum,and this is the calcium channel (non-patent documents 1-5). Furthermore,the present inventors have clarified that the IP3 receptor is themolecule involved in development and differentiation, neural plasticityand various signal transduction (non-patent documents 6-11). Inaddition, they have clarified that the IP3 receptor is also bound to theCa²⁺ channel on the cell membrane surface (non-patent document 12).

2-Aminoethyl diphenylborinate (2-APB: C₆H₅B (OCH₂CH₂NH₂)C₆H₅) has beeninternationally recognized as an IP3 receptor inhibitor, and is soldfrom Sigma. It decreases intracellular calcium concentration byinhibiting SOCE. The present inventors have synthesized and foundcompounds that control intracellular calcium concentration (patentdocument 1, patent document 2, Japanese patent application No.2008-028152).

It has been clarified that the causes of intractable diseases such asAlzheimer's disease, Parkinson's disease, Celiac disease, cataract, madcow disease, congenital lamellar ichthyosis, congenital hemostaticdisorder and the like are based on the abnormal cross-linking reactionof proteins (non-patent document 13, non-patent document 14). Inaddition, it has also been clarified that the cause of Huntington'sdisease is abnormal aggregation of polyglutamine (non-patent document15).

Transglutaminase is an enzyme activated by the presence of Ca²⁺, and itsinvolvement in neurological diseases such as Alzheimer's disease,Parkinson's disease, Huntington's disease and the like has recently beenknown. Therefore, novel inhibitors thereof are considered to beeffective as therapeutic drugs for the diseases (non-patent document 16,non-patent document 17). A reaction forming an isopeptide bond bydeammoniation of an amide group of glutamine and an amino group oflysine is the main reaction of protein cross-linking. The mechanism bywhich an inhibitor of enzyme transglutaminase causing the reaction iseffective for the aforementioned neurological diseases has beenclarified (non-patent document 19). As a basis, while many studies havebeen made based on the above to develop inhibitors of transglutaminaseas therapeutic drugs for intractable diseases such as Alzheimer'sdisease, Huntington's disease, Parkinson's disease and the like(non-patent documents 17-23), a boron compound having a transglutaminaseinhibitory activity has not been reported heretofore.

DOCUMENT LIST Patent Documents

-   patent document 1: WO03/033002 (U.S. Pat. No. 7,217,701)-   patent document 2: WO2007/061074

Non-Patent Documents

-   non-patent document 1: Furuichi, T. et al. Primary structure and    functional expression of the inositol 1,4,5-trisphosphate-binding    protein P400. Nature. 1989 Nov. 2; 342(6245):32-8.-   non-patent document 2: Miyawaki, A. et al. Expressed cerebellar-type    inositol 1,4,5-trisphosphate receptor, P400, has calcium release    activity in a fibroblast L cell line. Neuron. 1990 July; 5(1):11-8.-   non-patent document 3: Maeda, N. et al. Structural and functional    characterization of inositol 1,4,5-trisphosphate receptor channel    from mouse cerebellum. J Biol. Chem. 1991 Jan. 15; 266(2):1109-16.-   non-patent document 4: Kume, S. et al. The Xenopus 1P3 receptor:    structure, function, and localization in oocytes and eggs. Cell.    1993 May 7; 73(3):555-70.-   non-patent document 5: Yamamoto-Hino, M. et al. Cloning and    characterization of human type 2 and type 3 inositol triphosphate    receptors. Receptors and Channels. 1994; 2: 9-22.-   non-patent document 6: Miyazaki, S. et al. Block of Ca²⁺ wave and    Ca²⁺ oscillation by antibody to the inositol 1,4,5-trisphosphate    receptor in fertilized hamster eggs. Science. 1992 Jul. 10;    257(5067):251-5.-   non-patent document 7: Kume, S. et al. Role of inositol    1,4,5-trisphosphate receptor in ventral signaling in Xenopus    embryos. Science. 1997 Dec. 12; 278(5345):1940-3.-   non-patent document 8: Takei, K. et al. Regulation of nerve growth    mediated by inositol 1,4,5-trisphosphate receptors in growth cones.    Science. 1998 Nov. 27; 282(5394):1705-8.-   non-patent document 9: Nishiyama, M. et al. Calcium stores regulate    the polarity and input specificity of synaptic modification. Nature.    2000 Nov. 30; 408(6812):584-8.-   non-patent document 10: Bosanac, I. et al. Structure of the inositol    1,4,5-trisphosphate receptor binding core in complex with its    ligand. Nature. 2002 Dec. 12; 420(6916):696-700. Epub 2002 Nov. 17.-   non-patent document 11: Matsumoto, M. et al. Ataxia and epileptic    seizures in mice lacking type 1 inositol 1,4,5-trisphosphate    receptor. Nature. 1996 Jan. 11; 379(6561):168-71.-   non-patent document 12: Boulay, G. et al. Modulation of Ca(2+) entry    by polypeptides of the inositol 1,4,5-trisphosphate receptor (IP3R)    that bind transient receptor potential (TRP): evidence for roles of    TRP and IP3R in store depletion-activated Ca(2+) entry. Proc Natl    Acad Sci USA. 1999 Dec. 21; 96(26): 14955-60.-   non-patent document 13: Nobuyuki Nukina, Toru Nishikawa Experiment    Medicine 25, No 13, (extra edition), page 20-29 (2007)-   non-patent document 14: Hartley M Dean et al. Transglutaminase    induces protofibril-like amyloid β-protein assemblies that are    protease-resistant and inhibit long-term potentiation. J. Biol.    Chem. 2008 283: 16790-16800.-   non-patent document 15: Thomas M. Jeitner, et al. Increased levels    of γ-glutamylamines in Huntingtondisease CSF. J. Neurochemistry 2008    Apr. 1; 106(1):7-44.-   non-patent document 16: Kim, S. Y, et al. Transglutaminases in    disease. Neurochem Int. 2002 January; 40(1):85-103.-   non-patent document 17: Hoffner G, and Djian P. Transglutaminase and    diseases of the central nervous system. Front Biosci. 2005 Sep. 1;    10:3078-92.-   non-patent document 18: Duval E, et al. Structure-activity    relationship study of novel tissue transglutaminase inhibitors.    Bioorg Med Chem. Lett. 2005 Apr. 1; 15(7):1885-9.-   non-patent document 19: L. Lorand Neurodegenerative diseases and    transglutaminase. Proc Natl Acad Sci USA. 1996 Dec. 10;    93(25):14310-3.-   non-patent document 20: Mastroberardino P G, et al. ‘Tissue’    transglutaminase ablation reduces neuronal death and prolongs    survival in a mouse model of Huntington's disease. Cell Death    Differ. 2002 September; 9(9):873-80.-   non-patent document 21: Grierson A J, et al. Three different human    tau isoforms and rat neurofilament light, middle and heavy chain    proteins are cellular substrates for transglutaminase. Neurosci    Lett. 2001 Jan. 26; 298(1): 9-12.-   non-patent document 22: Watts R E, et al. Structure-activity    relationship analysis of the selective inhibition of    transglutaminase 2 by dihydroisoxazoles. J Med. Chem. 2006 Dec. 14;    49(25): 7493-501.-   non-patent document 23: Karpuj M V, et al. Prolonged survival and    decreased abnormal movements in transgenic model of    Huntingtondisease, with administration of the transglutaminase    inhibitor cystamine. Nat. Med. 2002 February; 8(2): 143-9.

SUMMARY OF THE INVENTION Problems to be Solved by the Invention

The present invention aims to develop a prophylaxis and/or therapeuticdrug for diseases caused by cross-linking abnormality of protein(Alzheimer's disease, Parkinson's disease, Celiac disease, cataract, madcow disease, congenital lamellar ichthyosis, congenital hemostaticdisorder etc.).

Means of Solving the Problems

The present inventors have conducted intensive studies in an attempt tosolve the aforementioned problems and found that a series of boroncompounds, particularly the compounds represented by the followingformulas (1)-(13) (hereinafter to be also simply referred to ascompounds (1)-(13)), inhibit cross-linking of protein, and the compoundscan be used as prophylactic and/or therapeutic drugs for diseases causedby abnormal cross-linking of proteins.

Accordingly, the present invention provides the following.

[1] A compound represented by any of the following formulas (1)-(13) ora pharmaceutically acceptable salt thereof;R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—[—X—B(ZR₁)—Y—]_(n)—R₄  (2)R₃—[—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (3)R₃—[—X—B(ZR₁)—]_(n)—R₄  (4)R₃—[—B(ZR₂)—W—]_(n)—R₄  (5)R₃—X—B(ZR₁)-T[B(ZR₂)—W—R₄]₂  (6)R₃—B(OH)₂  (7)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)R₃—B(R₁)—O—B(R₂)—R₄  (9)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—]_(n)—R₄  (10)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W-Q-]_(n)—R₄  (11)R₃—[—O—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (12)[R₃—X—B(ZR₁)—Y]₂B(ZR₂)  (13)wherein B is a boron atom,Z is O or S,R₁ and R₂ are independently a group selected from H, —(CH₂)_(m)—NR₅R₆,—CO—(CH₂)_(m)—NR₇R₉, —COCH(NH₂)—R₉, —CH₂CH(NH₂)—R₁₀, —CHR₁₁R₁₂,—COCH(—NR₁₃R₁₄)—R₁₅, —COCH(NH₂)—(CH₂)_(m)NHCR₁₈NH₂,—COCH(NH₂)—(CH₂)_(m)—COR₁₉, —COR₂₀, —(CH₂)_(m)—R₂₂, —O(CH₂)_(m)NH₂,—COCH(NH₂)—(CH₂)_(m)—R₂₃, —(CH₂CH₂NH)₂—R₂₃,

and heterocyclylalkyl, or when R₁ and R₂ are present in plurality, R₁may be bonded to R₁, R₂ may be bonded to R₂, or R₁ may be bonded to R₂,R₅, R₆, R₇, R₈, R₉, R₁₀, R₁₁, R₁₂, R₁₃, R₁₄, R₁₅, R₁₉, R₂₀ and R₂₂ areindependently H, or each is a substituted or unsubstituted alkyl,alkenyl, alkynyl, cycloalkyl, cycloalkenyl, aryl, arylalkyl,heterocyclyl, amino, aminoalkylcarbonyl, hydroxy, aromatic group orheterocyclylalkyl,R₁₈ is oxo or ═NH,Q is a group represented by —R₁₆—O—R₁₇—, —R₂₁—O— or —O— (wherein R₁₆,R₁₇ and R₂₁ mean a single bond or lower alkylene),R₂₃ is a fluorescence group,m is an integer of 1 to 5,R₃ and R₄ are H, OH, CH₂OH, CH₂OCH₂OCH₃, cyano or aryloxy, or each is asubstituted or unsubstituted alkyl or aryl,T is a substituted or unsubstituted aryl,X, Y and W are independently groups containing aromatic series or fattyseries, andn is an integer of 1 to 100.[2] A compound represented by any of the following formulas (1)-(13) ora pharmaceutically acceptable salt thereof;R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—[—X—B(ZR₁)—Y—]_(n)—R₄  (2)R₃—[—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (3)R₃—[—X—B(ZR₁)—]_(n)—R₄  (4)R₃—[—B(ZR₂)—W—]_(n)—R₄  (5)R₃—X—B(ZR₁)-T[B(ZR₂)—W—R₄]₂  (6)R₃—B(OH)₂  (7)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)R₃—B(R₁)—O—B(R₂)—R₄  (9)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—]_(n)—R₄  (10)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W-Q-]_(n)—R₄  (11)R₃—[—O—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (12)[R₃—X—B(ZR₁)—Y]₂B(ZR₂)  (13)wherein B is a boron atom,Z is O or S,R₁ and R₂ are independently a group selected from H, —(CH₂)_(m)—NR₅R₆,—CO—(CH₂)_(m)NR₇R₈, —COCH(NH₂)—R₉, —CH₂CH(NH₂)—R₁₀, —CHR₁₁R₁₂,—COCH(—NR₁₃R₁₄)—R₁₅, —COCH(NH₂)— (CH₂)_(m)NHCR₁₈NH₂,—COCH(NH₂)—(CH₂)_(m)—COR₁₉, —COR₂₀, —(CH₂)_(m)—R₂₂, —O(CH₂)_(m)NH₂,—COCH(NH₂)—(CH₂)_(m)—R₂₃, —(CH₂CH₂NH)₂—R₂₃,

and heterocyclylalkyl, or when R₁ and R₂ are present in plurality, R₁may be bonded to R₁, R₂ may be bonded to R₂, or R₁ may be bonded to R₂,R₅, R₆, R₇, R₈, R₉, R₁₀, R₁₁, R₁₂, R₁₃, R₁₄, R₁₅, R₁₉, R₂₀ and R₂₂ areindependently H, or each is a substituted or unsubstituted alkyl,alkenyl, alkynyl, cycloalkyl, cycloalkenyl, aryl, arylalkyl,heterocyclyl, amino, aminoalkylcarbonyl, hydroxy, aromatic group orheterocyclylalkyl, R₁₈ is oxo or ═NH,Q is a group represented by —R₁₆—O—R₁₁—, —R₂₁—O—, or —O— (wherein R₁₆,R₁₇ and R₂₁ mean a single bond or lower alkylene),R₂₃ is a fluorescence group,m is an integer of 1 to 5,R₃ and R₄ are H, OH, CH₂OH, CH₂OCH₂OCH₃, cyano or aryloxy, or each is asubstituted or unsubstituted alkyl or aryl,T is a substituted or unsubstituted aryl,X, Y and W are independently groups containing aromatic series or fattyseries, andn is an integer of 1 to 100,excluding a compound represented by the following formula (Ia)

whereinB is a boron atom,B is an oxygen or sulfur atom,J¹ and J³ are each independently a monocyclic aromatic group, apolycyclic aromatic group, or a heterocyclic group containing at leastone hetero atom selected from an oxygen atom, a nitrogen atom and asulfur atom,J² is a hydrogen atom; —(CH₂)_(D)—NJ⁴J⁵ wherein D is an integer of 1-4,J⁴ and J⁵ are independently a hydrogen atom, or C₁₋₄ alkyl substitutedor unsubstituted by an amino group, a mono or di-C₁₋₄ alkylamino groupor a phenyl group, or J⁴ and J⁵ form, together with the nitrogen atombonded thereto, a 5- or 6-membered cyclo ring); —CO—(CH₂)_(D)—NJ⁴J⁵wherein D, J⁴ and J⁵ are as defined above; —COCH(NH₂)J⁶ wherein J⁶ is anamino acid residue, or —(CH₂)_(D′)NH₂ wherein D′ is an integer of 1 to3; —CHJ⁷J⁸ wherein J⁷ and J⁸ are independently an amino group, C₁₋₄alkyl substituted or unsubstituted by a mono or di(C₁₋₄ alkylsubstituted or unsubstituted by an amino group)amino group or phenylgroup, or phenyl substituted by pyridyl or a C₁₋₃ alkoxy group;—CH₂CH(NH₂)-J⁶ wherein J⁹ is phenyl, or C₁₋₄ alkyl substituted byphenyl; quinolyl or isoquinolyl substituted by a alkyl group; or C₁₋₄alkyl substituted by a pyridyl group, a piperidino group or apyrrolidinyl group, andU is a monocyclic aromatic group, a polycyclic aromatic group or aheterocyclic group, which is the same as or different from J¹ and J³, ora bifunctional group having a monocyclic aromatic group, a polycyclicaromatic group or a heterocyclic group bonded to both sides thereof viaa group selected from the group consisting of a single bond, O, CH₂, S,SO₂, CH₂OCH₂, OCH₂, OCH₂CH₂OCH₂, OCH₂OCH₂CH₂ and CH₂OCH₂CH₂, and acompound represented by the following formula (Ib)

wherein J¹⁰ is(1) a hydrogen atom,(2) —(CH₂)_(D″)NJ¹¹J¹² wherein D″ is an integer of 1 to 3, J¹¹ and J¹²are each independently a hydrogen atom, C₁₋₄ alkyl, C₅₋₆ monocycliccarbocycle, C₁₋₄ alkyl substituted by C₅₋₆ monocyclic carbocycle, or 5-or 6-membered monocyclic heterocycle,the carbon atom in —(CH₂)_(D″)— is optionally substituted by 1 or 2 J¹³,and further, said carbocycle and heterocycle are optionally substitutedby 1 or 2 J¹⁶,J¹³ is (a) C₁₋₈ alkyl, (b) carboxyl, (c) C₁₋₄ alkoxycarbonyl, (d) keto,(e) C₅₋₆ monocyclic carbocycle, (f) guanidino(C₁₋₂)alkyl, (g) C₁₋₆ alkylsubstituted by C₅₋₆ monocyclic carbocycle, (h) C₁₋₂ alkyl substituted by4-chlorophenoxy, or (i) C₁₋₄ alkyl substituted by di(C₁₋₄ alkylamino,(3) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by C₅₋₆ monocycliccarbocycle, wherein said carbocycle is optionally substituted by 1 to 5J¹⁶, and further, said C₁₋₆ alkyl or C₂₋₆ alkenyl is optionallysubstituted by 1 or 2 J¹⁹,(4) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by 5- or 6-memberedmonocyclic heterocycle, wherein said heterocycle is optionallysubstituted by 1 to 5 J¹⁶, and further, said C₁₋₆ alkyl or C₂₋₆ alkenylis optionally substituted by 1 or 2 J¹⁹, andJ¹⁹ is C₁₋₄ alkyl or C₂₋₄ alkenyl,(5) a —CHJ¹⁴J¹⁵ group wherein J¹⁴ and J¹⁵ are each independently(i) C₅₋₆ monocyclic carbocycle,(ii) 5- or 6-membered monocyclic heterocycle,(iii) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by C₅₋₆ monocycliccarbocycle, or(iv) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by 5- or 6-memberedmonocyclic heterocycle, wherein said carbocycle and heterocycle areoptionally substituted by 1 to 5 J¹⁶, or(6) 5,6,7,8-tetrahydroquinolin-8-yl,J¹⁶ is (a) C₁₋₄ alkyl, (b) C₁₋₄ alkoxy, (c) a halogen atom, (d) —CF₃,(e) nitro, (f) C₅₋₆ monocyclic carbocycle, (g) C₁₋₄ alkyl substituted byC₅₋₆ monocyclic carbocycle, (h) amino, (i) —NHCO(C₁₋₄ alkyl), or (j)C₁₋₄ alkoxycarbonyl,G is Cyc₁ or hydroxy, Cyc₁ is C₅₋₁₀ monocyclic or bicyclic carbocycle,or 5- to 10-membered monocyclic or bicyclic heterocycle, wherein saidcarbocycle and heterocycle are optionally substituted by 1 to 5 J¹⁷,Cyc₂ is C₅₋₁₀ monocyclic or bicyclic heterocycle or 5- to 10-memberedmonocyclic or bicyclic heterocycle, wherein said carbocycle andheterocycle are optionally substituted by 1 to 5 J¹⁸, J¹⁷ and J¹⁸ areeach independently

-   (a) C₁₋₄ alkyl,-   (b) C₂₋₄ alkenyl,-   (c) C₁₋₄ alkoxy,-   (d) a halogen atom,-   (e) —CF₃,-   (f) alkylthio,-   (g) amino,-   (h) (C₁₋₄ alkyl)amino,-   (i) di(C₁₋₄ alkyl)amino,-   (j) formyl,-   (k) phenyl,-   (l) phenoxy,-   (m) hydroxy(C₁₋₂)alkyl,-   (n) (C₅₋₁₀ monocyclic or bicyclic carbocycle)-O—(C₁₋₂)alkyl,-   (o) C₁₋₄ alkoxycarbonylvinyl,-   (p) C₁₋₂ alkyl substituted by a group selected from —O—(C₁₋₂    alkylene)-phenyl (said phenyl is optionally substituted by 1 to 3    C₁₋₄ alkoxy), —O—CONH-phenyl (said phenyl is optionally substituted    by 1 to 3 C₁₋₄ alkyl, nitro or C₁₋₄ alkoxycarbonyl), or    —O—CONH—(C₁₋₄)alkyl (said alkyl is optionally substituted by 1 to 3    C₁₋₄ alkyl, carboxyl or C₁₋₄ alkoxycarbonyl),-   (q) phenylthio,-   (r) —CON(C₁₋₄ alkyl)₂.-   (s) —SO₂N(C₁₋₄ alkyl)₂,-   (t) alkoxy(C₁₋₂)alkyl,-   (u) C₁₋₄ alkoxycarbonyloxy(C₁₋₂)alkyl,

carbocycle, phenyl, Cyc₁ and Cyc₂ in J¹⁷ and J¹⁸ are optionallysubstituted by 1 or 2 J¹⁸, or J¹⁷ and J¹⁸ in combination optionally show—O—, and J¹⁸ and J¹⁹ in combination optionally show a single bond,d′₁ is an integer of 1 to 4,d′₂ is an integer of 1 to 4,d′₃ is an integer of 1 to 4, andE is a single bond or C₁₋₄ alkylene substituted or unsubstituted by C₅₋₆monocyclic carbocycle.[3] The compound of [2] represented by the following formula (4′) or(8′)R₃′—[X′—B(ZR₁′)—]_(n)—R₄′  (4′)R₃′—B(ZR₁′)—X′—B(ZR₂′)—R₄′  (8′)wherein B is a boron atom,Z is O or S,R₁′ and R₂′ are H, —(CH₂)_(m)—NR₅′R₆′, —COCH(NH₂)—(CH₂)_(m)NHCONH₂ or—COCH(NH₂)—(CH₂)_(m)—COR₁₉′, wherein R₅′, R₆′, R₁₁′, R₁₂′ and R₁₉′ areindependently H, or each is a substituted or unsubstituted amino,heterocyclyl or aryloxy,R₃′ and R₄′ are H, aryl or heterocyclyl,X′ is a substituted or unsubstituted aromatic group,m is an integer of 1 to 5, andn is an integer of 1 to 100, or a pharmaceutically acceptable saltthereof.[4] The compound of [2] or [3], which is any of

wherein n is an integer of 1 to 100, or a pharmaceutically acceptablesalt thereof.[5] A protein cross-linking inhibitor comprising the compound of [2] to[4] or a pharmaceutically acceptable salt thereof.[6] The inhibitor of [5], wherein the compound is represented by theformula (1) or (8)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)wherein each symbol is as defined in [2].[7] A prophylactic and/or therapeutic drug for a disease caused bycross-linking of protein, comprising the compound of [2] to [4] or apharmaceutically acceptable salt thereof.[8] The prophylactic and/or therapeutic drug of [7], wherein thecompound is represented by the formula (1) or (8)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)wherein each symbol is as defined in [2].[9] The prophylactic and/or therapeutic drug of [7] or [8], wherein thedisease caused by cross-linking of protein is selected from Alzheimer'sdisease, Parkinson's disease, Celiac disease, cataract, mad cow disease,congenital lamellar ichthyosis and congenital hemostatic disorder.[10] A method of preventing and/or treating a disease caused bycross-linking of protein, comprising administering an effective amountof the compound of [2] to [4] or a pharmaceutically acceptable saltthereof to a subject.[11] The method of [10], wherein the compound is represented by theformula (1) or (8)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)wherein each symbol is as defined in [2].[12] The method of [10] or [11], wherein the disease caused bycross-linking of protein is selected from Alzheimer's disease,Parkinson's disease, Celiac disease, cataract, mad cow disease,congenital lamellar ichthyosis and congenital hemostatic disorder.[13] The compound of [2] to [4] to be used for the prophylaxis and/ortreatment of disease caused by cross-linking of protein, orpharmaceutically acceptable salts thereof.[14] The compound of [13] which is represented by the formula (1) or (8)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)wherein each symbol is as defined in [2], or a pharmaceuticallyacceptable salt thereof.[15] The compound of [13] or [14], wherein the disease caused bycross-linking of protein is selected from Alzheimer's disease,Parkinson's disease, Celiac disease, cataract, mad cow disease,congenital lamellar ichthyosis and congenital hemostatic disorder, or apharmaceutically acceptable salt thereof.

Effect of the Invention

The present invention can provide a prophylactic and/or therapeutic drugfor the diseases based on an abnormal cross-linking reaction of proteinsuch as Alzheimer's disease, Parkinson's disease, Celiac disease,cataract, mad cow disease, congenital lamellar ichthyosis, congenitalhemostatic disorder and the like.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a drawing showing an Icrac inhibitory effect of 162AE, whereinthe vertical axis shows a relative electric current when the amount ofIcrac immediately before acting 162AE (120 sec) is 1, and the horizontalaxis shows time (seconds).

FIG. 2 is a drawing showing an Icrac inhibitory effect of 163AE, and thevertical axis and the horizontal axis show the same as in FIG. 1.

FIG. 3 is a drawing showing a dose inhibition curve relating to theinhibitory effect of 162AE and 163AE on Icrac, wherein the vertical axisshows the amount in percentage of Icrac when the inhibitor was usedrelative to the amount of Icrac without the inhibitor as 100%, and thehorizontal axis shows the concentration (M) of the inhibitor.

DESCRIPTION OF EMBODIMENTS

In the present invention, the cross-linking of protein means the statewhere a new bond of protein chain is formed in a molecule or betweenmolecules (covalent bond, ionic bond, coordinate bond, hydrogen bondetc.), and a bridge is built.

In addition, polyglutamine aggregation means formation of assembly ofpolyglutamine (polymerization and/or specific aggregate).

Abnormal aggregation of polyglutamine is one example of cross-linkingabnormalities of protein. An abnormal cross-linking of protein occursdue to abnormal transglutaminase activity that depends on calciumconcentration.

The present invention relates to a protein cross-linking inhibitorcontaining a compound represented by any of the following formulas(1)-(13).R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (1)R₃—[—X—B(ZR₁)—Y—]_(n)—R₄  (2)R₃—[—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (3)R₃—[—X—B(ZR₁)—]_(n)—R₄  (4)R₃—[—B(ZR₂)—W—]_(n)—R₄  (5)R₃—X—B(ZR₁)-T[B(ZR₂)—W—R₄]₂  (6)R₃—B(OH)₂  (7)R₃—B(ZR₁)—X—B(ZR₂)—R₄  (8)R₃—B(R₁)—O—B(R₂)—R₄  (9)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—]_(n)—R₄  (10)R₃—[—X—B(ZR₁)—Y—B(ZR₂)—W-Q-]_(n)—R₄  (11)R₃—[—O—X—B(ZR₁)—Y—B(ZR₂)—W—]_(n)—R₄  (12)[R₃—X—B(ZR₁)—Y]₂B(ZR₂)  (13)

In the formula.

B is a boron atom,

Z is O or S,

R₁ and R₂ are independently a group selected from H, —(CH₂)_(m)—NR₅R₆,—CO—(CH₂)_(m)—NR₇R₈, —COCH(NH₂)—R₉, —CH₂CH(NH₂)—R₁₀, —CHR₁₁R₁₂,—COCH(—NR₁₃R₁₄)—R₁₅, —COCH(NH₂)—(CH₂)_(m)NHCR₁₈NH₂,—COCH(NH₂)—(CH₂)_(m)—COR₁₉, —COR₂₀, —(CH₂)_(m)—R₂₂, —O(CH₂)_(m)NH₂,—COCH(NH₂)—(CH₂)_(m)—R₂₃, —(CH₂CH₂NH)₂—R₂₃,

and heterocyclylalkyl, or when R₁ and R₂ are present in plurality, R₁may be bonded to R₁, R₂ may be bonded to R₂, or R₁ may be bonded to R₂,R₅, R₆, R₇, R₈, R₉, R₁₀, R₁₁, R₁₂, R₁₃, R₁₄, R₁₅, R₁₉, R₂₀ and R₂₂ areindependently H, or each is a substituted or unsubstituted alkyl,alkenyl, alkynyl, cycloalkyl, cycloalkenyl, aryl, arylalkyl,heterocyclyl, amino, aminoalkylcarbonyl, hydroxy, aromatic group orheterocyclylalkyl,R₁₈ is oxo or ═NH,Q is a group represented by —R₁₆—O—R₁₇—, —R₂₁—O— or —O— (wherein R₁₆,R₁₇ and R₂₁ mean a single bond or lower alkylene),R₂₃ is a fluorescence group,m is an integer of 1 to 5,R₃ and R₄ are H, OH, CH₂OH, CH₂OCH₂OCH₃, cyano or aryloxy, or each is asubstituted or unsubstituted alkyl or aryl,T is a substituted or unsubstituted aryl,X, Y and W are independently groups containing aromatic series or fattyseries, andn is an integer of 1 to 100.

R₁ and R₂ are preferably independently a group selected from H,—(CH₂)_(m)—NR₅R₆, —CH₂CH(NH₂)—R₁₀, —CHR₁₁R₁₂,—COCH(NH₂)—(CH₂)_(m)—COR₁₉, —COR₂₀, —(CH₂)_(m)—R₂₂,—COCH(NH₂)—(CH₂)_(m)—R₂₃ and heterocyclylalkyl.

R₃ and R₄ are preferably independently H, or a substituted orunsubstituted aryl.

When n is 2 to 100, repeat units may be bonded to each other at bothends, and may be bonded by R₁ and R₂.

In the present specification, preferable examples of alkyl includemethyl, ethyl, propyl, butyl and isomers thereof.

In the present specification, “heterocyclyl” means 5- to 10-memberedsaturated or unsaturated monocycle containing 1 to 4 hetero atoms (anitrogen atom, a sulfur atom, an oxygen atom) or a fused ring thereof.For example, pyrrole, imidazole, triazole, tetrazole, pyrazole,pyridine, pyrazine, piperidine, piperazine, pyrrolidine, pyrimidine,pyridazine, furan, pyran, thiophene, thiin, oxazole, isoxazole,thiazole, isothiazole, indole, isoindole, benzofuran, isobenzofuran,benzothiophene, isobenzothiophene, indazole, quinoline, isoquinoline,quinoxaline, quinazoline, cinnoline, benzooxazole, benzothiazole,benzoimidazole, chromene, indoline, isoindoline, dihydrobenzofuran,dihydrobenzothiophene, dihydroindazole, tetrahydroquinoline,tetrahydroisoquinoline, tetrahydroquinoxaline, tetrahydroquinazoline,tetrahydrocinnoline and the like can be mentioned.

Here, heterocyclylalkyl means the aforementioned alkyl moietysubstituted by the aforementioned heterocyclyl moiety. Preferableexamples of heterocyclylalkyl include 2-pyridylmethyl.

In the present specification, preferable examples of alkenyl includeethenyl, propenyl, butenyl, and isomers thereof and the like.

In the present specification, preferable examples of alkynyl includeethynyl, propynyl, butynyl, and isomers thereof and the like.

In the present specification, “cycloalkyl” means cyclic saturatedhydrocarbon. Examples of cycloalkyl include 3- to 10-membered,preferably 5- or 6-membered, cycloalkyl such as cyclopentyl andcyclohexyl.

In the present specification, the “cycloalkenyl” means cyclicunsaturated hydrocarbon having 1 or 2 carbon-carbon double bonds.

Preferable examples of cycloalkenyl include 5- or 6-memberedcycloalkenyl, for example, cyclopentenyl, cyclohexenyl and the like.

In the present specification, “aryl” means an atomic group obtained byremoving one hydrogen atom from aromatic hydrocarbon. Examples of arylinclude a substituted or unsubstituted phenyl, naphthyl, anthryl and thelike.

In the present specification, “arylalkyl” means the aforementioned alkylmoiety substituted by 1 or plural aforementioned aryl moieties.Preferable examples of arylalkyl include benzyl and phenylethyl.

In the present specification, aryl of the “aryloxy” is as defined above.Preferable examples of aryloxy include phenoxy.

The aforementioned alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl,aryl, arylalkyl, aryloxy, heterocyclyl and heterocyclylalkyl may havesubstituent(s) at substitutable position(s). While the number of thesubstituents is not particularly limited, it is preferably 1 to 3.Specific examples of the substituent include halogen (e.g., fluorine,chlorine), optionally substituted hydroxy (e.g., hydroxy, alkoxy (e.g.,methoxy, ethoxy)), optionally substituted methyl (e.g., methyl,trifluoromethyl), optionally substituted amino, carboxyl, optionallysubstituted phenyl (e.g., phenyl, naphthyl), thiol, optionallysubstituted amide (e.g., carbonamide), aminoalkylcarbonyl (e.g.,aminoethylcarbonyl), thioalkyl (e.g., thiomethyl), and cyano. Theoptionally substituted amino may have substituent(s) at substitutableposition(s). Specific examples of the substituent include aminoalkyl.

In the present specification, “lower alkylene” means straight chain orbranched alkylene having a carbon number of 1 to 6, preferably 1 to 4,and preferably includes methylene, ethylene and propylene.

In the present specification, “aminoalkyl” means alkyl having an aminogroup, preferably aminoethyl.

In the present specification, the “fluorescence group” includesfluorescein such as fluorescein isothiocyanate (FITC) and the like,tetramethylrhodamine (TMeRH), cyanine (Cy2, Cy3, Cy5, Cy7 etc.),fluorescamine and the like. Particularly, FITC and TMeRH are preferable.

In the present specification, the aromatic group is a group derived fromaromatic hydrocarbon and heterocycle showing aromatic property, andmeans a group derived from monocyclic aromatic series (monocyclicaromatic group) and a group derived from polycyclic aromatic series(polycyclic aromatic group). The monocyclic aromatic group means asubstituted or unsubstituted phenyl or phenylene group. The phenylenegroup includes o-, m- and p-phenylene. Examples of the substituentinclude at least one substituent selected from the group consisting ofhalogen (e.g., fluorine, chlorine), halogenated C₁-C₄ alkyl, cyano,hydroxy, hydroxy C₁-C₄ alkyl, sulfanyl, amino, nitro, mono or di C₁-C₄alkylamino, carboxyl, C₁-C₄ alkylcarbonyl, C₁-C₄ alkylcarbonyloxy, C₁-C₄alkyl, C₂-C₄ alkenyl, C₂-C₄ alkynyl, cycloalkyl (as defined above),cycloalkenyl (as defined above), C₁-C₄ alkylthio, C₁-C₄ alkoxy, aryl (asdefined above), aryloxy (as defined above), amide and C₁-C₄ alkylamide,thiol and carbamoyl.

In the aforementioned groups, the C₁-C₄ alkyl moiety means a linear orbranched alkyl group having a carbon number of 1 to 4 (e.g., methyl,ethyl, propyl, butyl).

In the aforementioned group, the C₁-C₄ alkoxy moiety means a linear orbranched alkoxy group having a carbon number of 1 to 4 (e.g., methoxy,ethoxy).

In the aforementioned group, the C₂-C₄ alkenyl moiety means a linear orbranched alkenyl group having a carbon number of 1 to 4 (e.g., ethenyl,propenyl, butenyl).

In the aforementioned group, the C₂-C₄ alkynyl moiety means a linear orbranched alkynyl group having a carbon number of 1 to 4 (e.g., ethynyl,propynyl, butynyl).

In the aforementioned group, the aryl moiety is as defined above.

In the aforementioned group, examples of the substituted phenyl include,but are not limited to, mono, di or trifluorophenyl, methoxyphenyl,tolyl, xylyl, o-chlorotolyl, trifluoromethylphenyl, methoxyphenyl,tolyl, xylyl, o-chlorotolyl, trifluoromethylphenyl,2-methoxy-5-fluorophenyl, hydroxymethylphenyl, phenoxyphenyl and thelike. Examples of the substituted phenylene include, but are not limitedto, 5-methyl-m-phenylene, 5-methyl-p-phenylene and the like. Thepolycyclic aromatic group means a fused polycyclic hydrocarbon groupcomprised of a fused ring of 2 to 6, preferably 2 or 3, of 5-memberedand/or 6-membered monocyclic carbocycles. Examples include, but are notlimited to, substituted or unsubstituted naphthyl, anthryl, phenanthryl,indenyl, fluorenyl and the like. Here, examples of the substituentinclude the same substituents as recited above. Examples of the aromaticheterocyclic group include a 5-membered ring containing one hetero atomsuch as a furanyl group, a thiophenyl group, a pyrrolyl group and thelike, a 6-membered ring containing one hetero atom such as a pyridinylgroup and the like, a 5-membered ring containing two hetero atoms suchas an oxazolyl group, a thiazolyl group and the like, a 6-membered ringcontaining two hetero atoms such as a pyridazinyl group, a pyrimidinylgroup and the like, and a 5- to 7-membered ring containing at least onehetero atom, a bicyclic condensed hetero group containing one heteroatom such as an indolyl group, a quinolinyl group and the like, abicyclic condensed hetero group containing two hetero atoms such as aquinoxalinyl group and the like, a tricyclic condensed hetero groupcontaining one hetero atom such as an acrydinyl group and the like, abicyclic condensed hetero group containing two hetero atoms such as anindazolyl group and the like, and a polycyclic condensed hetero groupcontaining at least one hetero atom, and the like.

In the present specification, a group of aliphatic series (aliphaticgroup) is a group derived from saturated hydrocarbon (alkane) andunsaturated hydrocarbon (alkene, alkyne).

Particularly preferably, X, Y and W are groups containing aromaticseries or aliphatic series, monocyclic aromatic groups, such as

condensed aromatic groups having two or more rings, such as

aromatic groups wherein two aromatic groups are directly bonded, such as

substituted or unsubstituted aromatic groups wherein two aromatic groupsare bonded via O, CH₂, S, SO₂, CH₂OCH₂, OCH₂, OCH₂CH₂OCH₂, OCH₂OCH₂CH₂,CH₂OCH₂CH₂, CH₂OCH₂CH₂OCH₂ and the like, such as

and substituted or unsubstituted aliphatic groups such as (CH₂)₄ can bementioned.

More preferably, as X, Y, W, monocyclic aromatic groups, such as

condensed aromatic groups having two or more rings, such as

aromatic groups wherein two aromatic groups are directly bonded, such as

substituted or unsubstituted aromatic groups wherein an aromatic groupis bonded via O, CH₂O, CH₂OCH₂ and the like, such as

can be mentioned.

However the compound of the present invention excludes a compoundrepresented by the following formula (Ia)

and a compound represented by the following formula (Ib)

In the formula (Ia), B is a boron atom, and V is an oxygen or sulfuratom. J¹ and J³ are each independently a monocyclic aromatic group, apolycyclic aromatic group, or a heterocyclic group containing at leastone hetero atom selected from an oxygen atom, a nitrogen atom and asulfur atom.

J² is a hydrogen atom; —(CH₂)_(D)—NJ⁴J⁵ wherein D is an integer of 1-4,J⁴ and J⁵ are independently a hydrogen atom, or C₁₋₄ alkyl substitutedor unsubstituted by an amino group, a mono or di-C₁₋₄ alkylamino groupor a phenyl group, or J⁴ and J⁵ form, together with a nitrogen atombonded thereto, a 5-membered or 6-membered cyclo ring); —CO—(CH₂)J⁵wherein D, J⁴ and J⁵ are as defined above); —COCH(NH₂)J⁶ wherein J⁶ isan amino acid residue, or —(CH₂)_(D′)NH₂ wherein D′ is an integer of1-3; —CHJ⁷J⁶ wherein J⁷ and J⁸ are independently an amino group, C₁₋₄alkyl substituted or unsubstituted by a mono or di(C₁₋₄ alkylsubstituted or unsubstituted by an amino group)amino group or phenylgroup, or phenyl substituted by pyridyl or C₁₋₃ alkoxy group;—CH₂CH(NH₂)-J⁹ wherein J⁹ is C₁₋₄ alkyl substituted by phenyl orphenyl); quinolyl or isoquinolyl substituted by a C₁₋₄ alkyl group; orC₁₋₄ alkyl substituted by a pyridyl group, a piperidino group or apyrrolidinyl group.

U is a monocyclic aromatic group, a polycyclic aromatic group or aheterocyclic group, which is the same as or different from J¹ and J³, ora bifunctional group having a monocyclic aromatic group, a polycyclicaromatic group or a heterocyclic group bonded to both sides thereof viaa group selected from the group consisting of a single bond, O, CH₂, S,SO₂, CH₂OCH₂, OCH₂, OCH₂CH₂OCH₂, OCH₂OCH₂CH₂ and CH₂OCH₂CH₂.

A compound represented by the formula (Ia) to be excluded from thecompound of the present invention corresponds to a compound representedby the formula (I) disclosed in WO2007/061074. Therefore, the definitionof each substituent (functional group) in the formula (Ia) follows thedefinition described in the publication.

In the formula (Ib), J¹⁰ is any of the following (1)-(6).

(1) a hydrogen atom.

(2) —(CH₂)_(D″)—NJ¹¹J¹².

In the group, D″ is an integer of 1-3, J¹¹ and J¹² are eachindependently a hydrogen atom, C₁₋₄ alkyl, C₅₋₆ monocyclic carbocycle,C₁₋₄ alkyl substituted by C₅₋₆ monocyclic carbocycle, or 5- or6-membered monocyclic heterocycle.

The carbon atom in —(CH₂)_(D″)— is optionally substituted by 1 or 2 J¹³,and the carbocycle and heterocycle are optionally substituted by 1 or 2J¹⁶. J¹³ is (a) C₁₋₈ alkyl, (b) carboxyl, (c) alkoxycarbonyl, (d) keto,(e) C₅₋₆ monocyclic carbocycle, (f) guanidino(C₁₋₂)alkyl, (g) C₁₋₆ alkylsubstituted by C₅₋₆ monocyclic carbocycle, (h) C₁₋₂ alkyl substituted by4-chlorophenoxy, or (i) C₁₋₄ alkyl substituted by di(C₁₋₄ alkylamino.

(3) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by C₅₋₆ monocyclic carbocycle.

The carbocycle is optionally substituted by 1 to 5 J¹⁶, and the C₁₋₆alkyl or C₂₋₆ alkenyl is optionally substituted by 1 or 2 J¹⁹.

(4) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by 5- or 6-memberedmonocyclic heterocycle.

The heterocycle is optionally substituted by 1 to 5 J¹⁶, and the C₁₋₆alkyl and C₂₋₆ alkenyl are optionally substituted by 1 or 2 J¹⁹. J¹⁹ isC₁₋₄ alkyl or C₂₋₄ alkenyl.

(5) —CHJ¹⁴J¹⁵.

In the group, J¹⁴ and J¹⁵ are each independently

(i) C₅₋₆ monocyclic carbocycle,

(ii) 5- or 6-membered monocyclic heterocycle,

(iii) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by C₅₋₆ monocycliccarbocycle, or

(iv) C₁₋₆ alkyl or C₂₋₆ alkenyl substituted by 5- or 6-memberedmonocyclic heterocycle.

Moreover, the carbocycle and heterocycle are optionally substituted by 1to 5 J¹⁶.

(6) 5,6,7,8-tetrahydroquinolin-8-yl.

J¹⁶ is (a) C₁₋₄ alkyl, (b) C₁₋₄ alkoxy, (c) a halogen atom, (d) —CF₃,(e) nitro, (f) C₅₋₆ monocyclic carbocycle, (g) C₁₋₄ alkyl substituted byC₅₋₆ monocyclic carbocycle, (h) amino, (i) —NHCO(C₁₋₄ alkyl), or (j)C₁₋₄ alkoxycarbonyl.

G is Cyc₁ or hydroxy.

Cyc₁ is C₅₋₁₀ monocyclic or bicyclic carbocycle, or 5- to 10-memberedmonocyclic or bicyclic heterocycle, the carbocycle and heterocycle areoptionally substituted by 1 to 5 J¹⁷.

Cyc₂ is C₅₋₁₀ monocyclic or bicyclic heterocycle, or 5- to 10-memberedmonocyclic or bicyclic heterocycle. The carbocycle and heterocycle areoptionally substituted by 1 to 5 J^(n).

J¹⁷ and J¹⁸ are each independently

-   (a) C₁₋₄ alkyl,-   (b) C₂₋₄ alkenyl,-   (c) C₁₋₄ alkoxy,-   (d) a halogen atom,-   (e) —CF₃,-   (f) alkylthio,-   (g) amino,-   (h) (C₁₋₄ alkyl)amino,-   (i) di(C₁₋₄ alkyl)amino,-   (j) formyl,-   (k) phenyl,-   (l) phenoxy,-   (m) hydroxy (C₁₋₂) alkyl,-   (n) (C₅₋₁₀ monocyclic or bicyclic carbocycle)-O—(C₁₋₂)alkyl,-   (o) C₁₋₄ alkoxycarbonylvinyl,-   (p) C₁₋₂ alkyl substituted by group(s) selected from —O— (C₁₋₂    alkylene)-phenyl (said phenyl is optionally substituted by 1 to 3    C₁₋₄ alkoxy), —O—CONH-phenyl (said phenyl is optionally substituted    by 1 to 3 C₁₋₄ alkyl, nitro or C₁₋₄ alkoxycarbonyl), or    —O—CONH—(C₁₋₄)alkyl (said alkyl is optionally substituted by 1 to 3    C₁₋₄ alkyl, carboxyl or C₁₋₄ alkoxycarbonyl),-   (q) phenylthio,-   (r) —CON(C₁₋₄ alkyl)₂,-   (s) —SO₂N(C₁₋₄ alkyl)₂,-   (t) C₁₋₄ alkoxy (C₁₋₂) alkyl,-   (u) C₁₋₄ alkoxycarbonyloxy (C₁₋₂) alkyl,

The carbocycle, phenyl, Cyc₁ and Cyc₂ in J¹⁷ and J¹⁸ are optionallysubstituted by 1 or 2 J¹⁶, or J¹⁷ and J¹⁸ optionally show —O—, andfurther, J¹⁸ and J¹⁹ optionally show a single bond.

d′₁ is an integer of 1-4, d′₂ is an integer of 1-4, and d′₃ is aninteger of 1-4. E is a single bond or C₁₋₄ alkylene substituted orunsubstituted by C₅₋₆ monocyclic carbocycle.

A compound represented by the formula (Ib) to be excluded from thecompound of the present invention corresponds to a compound representedby the formula (I) disclosed in WO03/033002. Therefore, the definitionof each substituent (functional group) in the formula (Ib) follows thedefinition described in the publication.

The compounds of the aforementioned (1)-(13) in the present inventionspecifically include the following.

-   2-aminoethylthio bis(4-chloro-2-fluorophenyl)borane-   (4-(phenylglutamineboryl)phenyl)    (4′-(phenylhydroxyboryl)phenyl)ether-   bis(4,4′-(phenylhydroxyboryl)phenyl)ether-   poly(4,4′-biphenylene N-methylaminoethoxyborane)-   bis(4,4′-(phenylaminoethoxyboryl)phenyl)ether-   (4-(phenylasparagineboryl)phenyl)(4′-(phenylhydroxyboryl)phenyl)ether-   bis(3,3′-(phenylhydroxyboryl)benzyl)ether-   bis(3,3′-(phenylaminoethoxyboryl)benzyl)ether-   4,4′(phenyl-2-aminoethylthioboryl)diphenyl-   4,4′(phenyl-2-aminoethoxyboryl)diphenyl-   poly(2,5-dimethoxy-4-phenylborinic acid)-   poly(aminoethyl-2,5-dimethoxy-4-phenylborinate)-   poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylene hydroxyborane)-   poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylene    2-aminoethoxyborane)-   poly(4,4′-phenylenemethyleneoxymethylene    4,4′-phenylene-dimethylaminoethoxyborinic acid)-   poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylene    2-piperidinomethoxyborane)-   poly(1,4-phenyleneoxy-1,4-phenylenehydroxyborane)-   poly(aminoethoxyboryldiphenylether)-   poly(isopropoxyboryldiphenylether)-   poly(4,4′-diphenylether dimethylaminoethoxyborane)-   poly(4,4′-diphenylether-2-pyridyl-4-trifluoromethylphenylmethoxyborane)-   poly(4,4′-diphenylether-2-aminoethylthioborane)-   poly(phenylenemethyleneoxyphenylenehydroxyborane)-   poly(phenylenemethyleneoxyphenyleneaminoethoxyborane)-   poly(phenylenemethyleneoxyphenyleneaminoethylthioborane)-   poly(phenylenemethyleneoxyphenylenedimethylaminoethoxyborane)-   poly(4′-phenylhydroxyboranephenylenemethyleneoxyphenylenehydroxyborane    phenylenemethyleneoxymethylene)-   poly(phenylenemethyleneoxyphenyleneaminoethoxyboranephenylene-methyleneoxymethylenephenylene    aminoethoxyborane)-   poly(phenylenemethyleneoxyphenylenemethylaminoethoxyborane-phenylenemethyleneoxymethylenephenylenemethylaminoethoxyborane)-   poly(4,4′-biphenylene-hydroxyborane    1,4-phenylenemethyleneoxymethylenephenylenehydroxyborane)-   poly(4,4′-biphenylene 2-aminoethoxyborane    1,4-phenylene-methyleneoxymethylene 1,4-phenylene    2-aminoethoxyborane)-   di(3-chloro-4-methylphenyl)-2-aminoethylthioborane-   poly(2,5-dimethoxy-4-phenylene-hydroxyborane-1,4-phenylenehydroxyborane)-   polyaminoethyl(2,5-dimethoxy-4-phenylene)aminoethoxyboryl(1,4-phenylene)borinate-   poly(2-pyridylmethyl(2,5-dimethoxy-4-phenylene)    2-pyridylmethoxyborane-(1,4-phenylene)borinate)-   poly(4,4′-biphenylene-hydroxyborane 4,4′-diphenylether    hydroxyborane)-   poly(4,4′-biphenylene-dimethylaminoethoxyborane 4,4′-diphenylether    dimethylaminoethoxyborane)-   poly(4,4′-biphenylene-aminoethoxyborane-4,4′-diphenylether    aminoethoxyborane)-   poly(phenyleneaminoethoxyborane diphenylether-aminoethoxyborane)-   poly(phenyleneaminoethylthioborane    diphenylether-aminoethylthioborane)-   poly(phenylene 2-piperazinomethoxyborane diphenylether    2-piperidinomethoxyborane)-   poly(methylaminoethoxyborylphenylene    methylaminoethoxyboryldiphenylether)-   poly(pyrrolidinomethoxyborylphenylene    pyrrolidinomethoxyboryldiphenylether)-   poly(aminoethylaminoethoxyborylphenylene    aminoethylaminoethoxyboryldiphenylether)-   poly(metaphenylene-hydroxyborane-4,4′-diphenyletherhydroxyborane)-   poly(metaphenylene-2-piperidinemethoxyborane-4,4′-diphenylether-2-piperidinemethoxyborane)-   poly(metaphenylene-aminoethoxyborane-4,4′-diphenylether    aminoethoxyborane)-   poly(mataphenylene-methylaminoethoxyborane-4,4′-diphenylethermethylaminoethoxyborane)-   poly(metaphenylene-2-dimethylaminoethoxyborane-4,4′-diphenylether-2-dimethylaminoethoxyborane)-   poly(metaphenylene-2-pyridyl-trifluoromethylphenylmethoxyborane-4,4′-diphenylether-2-pyridyl-trifluoromethylphenylmethoxyborane)-   poly(metaphenylene-aminoethylthioborane-4,4′-diphenylether-aminoethylthioborane)-   poly(4,4′-diphenyletherhydroxyborane    phenylenemethyleneoxyphenylenehydroxyborane)-   poly(phenylenemethyleneoxyphenylene-aminoethoxyborane-4,4′-diphenyletheraminoethoxyborane)-   poly(phenyleneoxyphenylene-2-pyrrolidinemethoxyboryl-phenylenemethyleneoxyphenylene-2-pyrrolidinemethoxyborane)-   poly(phenylenemethyleneoxyphenylene-dimethylaminoethoxyborane-4,4′-diphenylether    dimethylaminoethoxyborane)-   poly(phenylenemethyleneoxyphenylene-2-pyridylmethoxyborane-4,4′-diphenylether-2-pyridylmethoxyborane)-   poly(4,4′-biphenylene-aminoethoxyborane-1,4-phenylene-methyleneoxy-1,4-phenylene-aminoethoxyborane)-   poly(4,4′-biphenylene-dimethylaminoethoxyborane-1,4-phenylene-methyleneoxyphenylenedimethylaminoethoxyborane)-   poly(4,4′-biphenylene-2-pyridylmethoxyborane-1,4-phenylene-methyleneoxy-1,4-phenylene-2-pyridylmethoxyborane)-   poly(4,4′-biphenylene-2-hydroxyethylaminoethoxyborane-1,4-phenylene-methyleneoxy-1,4-phenylene-2-hydroxyethylaminoethoxyborane)-   poly(4,4′-phenylene-methyleneoxymethylene-phenylene-hydroxyborane-4,4′-phenyleneoxyphenyleneborinic    acid)-   poly(phenylene-methyleneoxymethylene-phenylene-aminoethoxyborane-phenyleneoxyphenyleneaminoethoxyborane)-   poly(phenylene methyleneoxymethylene phenylene    dimethylaminoethoxyborane phenylene oxy phenylene    dimethylaminoethoxyborane)-   poly(phenylene methyleneoxymethylene phenylene aminoethylthioborane    phenylene oxy phenylene aminoethylthioborane)-   poly(diphenylene-methylaminoethoxyboryl-1,4-phenylene-methyleneoxymethylenephenylene-methylaminoethoxyborane)-   poly(1,4-phenylene-methyleneoxymethylenephenylenemethylaminoethoxyborane-1,4-phenylene-methylaminoethoxyborane)-   poly(1,4-phenylene-methyleneoxymethylenephenylene-aminoethylaminoethoxyborane-1,4-phenylene-aminoethylaminoethoxyborane)-   polytetramethyleneborinic acid-   2-dimethylaminoethyl bis(4-trifluoromethylphenyl)borinate-   1,3-dimethylaminopropyl bis(3-chloro-4-methylphenyl)borinate-   di(3-chloro-4-methylphenyl)(2,3-diaminopropionate-O,N)borane-   di(3-chloro-4-methylphenyl)piperazinoethoxyborane-   di(3-chloro-4-methylphenyl)piperidinoethoxyborane-   di(3-chloro-4-methylphenyl)-2-piperidinoethoxyborane-   bis(4-trifluoromethylphenyl)borinic acid-   di(3-fluoro-4-chlorophenyl)borinic acid-   2-aminoethyl-bis(3-chloro-4-fluorophenyl)borinate-   2-dimethylaminoethyl bis(3-chloro-4-fluorophenyl)borinate-   bis(4-chloro-2-fluorophenyl)borinic acid-   bis(3,4-difluorophenyl)borinic acid-   bis(3,4,5-trifluorophenyl)borinic acid-   bis(2,4-difluorophenyl)borinic acid-   bis(3-fluoro-4-chlorophenyl)borinic acid-   2-aminoethyl bis(4-chloro-2-fluorophenyl)borinate-   poly(4,4′-biphenylhydroxyborane)-   2-aminoethyl bis(3-chloro-4-fluorophenyl)borinate-   2-aminoethyl bis(3,4-difluorophenyl)borinate-   2-amino-1-phenylethyl bis(3,4-difluorophenyl)borinate-   aminoethyl bis(3,4,5-trifluorophenyl)borinate-   2-pyridylmethyl bis(3,4,5-trifluorophenyl)borinate-   aminoethyl bis(3,5-difluorophenyl)borinate-   dimethylaminoethyl bis(3,5-difluorophenyl)borinate-   aminoethyl bis(4-chloro-3-fluorophenyl)borinate-   dimethylaminoethyl bis(4-chloro-3-fluorophenyl)borinate-   di(3-fluoro-4-chlorophenyl)(2,4-diaminolactonate-O,N)borane-   di(3-fluoro-4-chlorophenyl)(glutaminate-O,N)borane-   bis(3-chloro-5-fluorophenyl)borinic acid-   bis(3-chloro-6-fluorophenyl)borinic acid-   aminoethyl bis(3-chloro-5-fluorophenyl)borinate-   aminoethyl bis(3-chloro-6-fluorophenyl)borinate-   methylaminoethyl bis(3-chloro-6-fluorophenyl)borinate-   bis(4-cyanophenyl)borinic acid-   aminoethyl bis(4-cyanophenyl)borinate-   2-pyridylmethyl bis(4-cyanophenyl)borinate-   benzylaminoethyl bis(4-cyanophenyl)borinate-   2-aminoethylthio bis(4-cyanophenyl)borane-   secondary-butyl phenyl borinic acid-   normal-butyl phenyl borinic acid-   tertiary-butyl phenyl borinic acid-   aminoethyl secondary-butyl phenylborinate-   aminoethyl tertiary-butyl phenylborinate-   aminoethyl normal-butyl phenylborinate-   1,4-bis(hydroxyphenylboryl)butane-   4-hydroxybutylphenylborinic acid-   bis(4-chlorophenyl)borinic acid-   bis(di(3-chloro-4-methylphenyl)boryloxyethyl)piperazine-   bis(3-chloro-4-methylphenyl 2-pyridylmethoxyborylphenyl)ether-   1,4-bis(phenyl-2-aminoethoxyboryl)benzene-   1,3-bis(phenyl-2-aminoethoxyboryl)benzene-   1,3-bis(phenylhydroxyboryl)benzene-   diphenyl(argininate-O,N)borane-   diphenyl(glutaminate-O,N)borane-   (2-phenylhydroxyborylbenzyl)(3-(phenylhydroxyboryl)benzyl)ether-   bis(3-chloro-4-methylphenyl hydroxyborylbenzyl)ether-   bis(phenyl 2-pyridyl-4-methoxyphenylmethoxyborylbenzyl)ether-   bis(3-chloro-4-methylphenyl) 2-pyridyl-4-methoxyphenylmethoxyborane-   1,4-bis(3-chloro-4-methylphenyl-2-aminoethoxyboryl)benzene-   di((phenylglycine-O,N boryl)phenyl)ether-   1,3,5-tri(phenylhydroxyboryl)benzene-   bis((4,4′-phenylaminoethoxyboryl)benzyl)ether-   1,3,5-tri(2-pyridyl-4-trifluoromethylphenylmethoxyphenylboryl)benzene-   (2-pyridyl-phenylmethoxyphenylboryl 2-benzyl)ether-   (2-pyridyl-4-trifluoromethylphenylmethoxyphenylboryl 2-benzyl)ether-   1,4-bis(phenylhydroxyboryl)naphthalene    diphenyl(asparaginate-O,N)borane-   bis((4,4′-phenylhydroxyboryl)benzyl)ether-   bis(2-pyridyl-4-trifluoromethylphenylmethoxyphenylboryl    4-benzyl)ether-   bis(4-chloro-3-methylphenylhydroxyboryl 4-benzyl)ether-   4,4′-phenylhydroxyboryl 4-biphenyl-   bis(4,4′-(1-naphthylhydroxyboryl)benzyl)ether-   bis(4-fluorophenylhydroxyboryl 4-benzyl)ether-   bis(4-trifluoromethylphenylhydroxyboryl 4-benzyl)ether-   bis(3-chloro-4-methylphenylhydroxyboryl 4-benzyl)ether-   (3-chloro-4-fluorophenyl)boronic acid-   1,4-bis(phenyl-2-aminoethoxyboryl) 2-methylbenzene    1,2-bis(phenylhydroxyboryl)benzene-   bis(2,2′-(phenyl-2-aminoethoxyboryl)benzyl)ether-   diphenyl-2-aminophenylthioborane-   2-aminoethylthiodiphenylborane-   di(4,4′-phenyldimethylaminoethoxyboryl)benzylether-   poly(4,4′-biphenylene-2-pyridyl-4-trifluoromethylphenylmethoxyborane-   4,4′-diphenylether 2-pyridyl-4-trifluoromethoxyborane)-   diphenyl 2-aminoethylaminoethyl borinate-   di(trifluoromethylphenyl) 2-pyridinomethylborinate-   di(3-chloro-6-methyl-phenyl)(argininate-O,N)borane-   poly(phenylenemethyleneoxyphenyleneaminoethoxyborane)-   poly(phenylenemethyleneoxyphenyleneaminoethylthioborane)-   dibutyl(alanine-O,N)borane-   di(3-chloro-6-methyl-phenyl)(citrullinate-O,N)borane-   FITC aminoethylaminoethyl diphenylborinate-   tetramethylrhodamine aminoethylaminoethyl diphenylborinate-   di(3-chloro-4-methylphenyl)N-methylpiperidinomethylborinate-   di(3-chloro-6-methylphenyl)benzylaminoethylborinate-   poly(4,4′-biphenylene-methylaminoethoxyborane 1,4-phenylene    methyleneoxymethylenephenylene-methylaminoethoxyborane)-   (4-(phenyl-dimethylaminoethoxyboryl)phenyl)-(4′-(methoxymethoxymethylphenyl-dimethylaminoethoxyboryl)phenyl)ether-   (4-(phenyl-N-methylaminoethoxyboryl)phenyl)-(4′-(methoxymethoxymethylphenyl-N-methylaminoethoxyboryl)phenyl)ether-   di((phenylglycine-O,N boryl)phenyl)ether-   diphenyl(glycylglutamine-O,N)borane-   di(3-chloro-6-methylphenyl)borinic acid-   bis(3,3′(phenyldimethylaminoethoxyboryl)benzyl)ether-   (3,3′-(phenylpiperazino-O,O-ethoxyboryl)benzyl)ether    diphenyl(2,3-diaminopropionate-O,N)borane-   diphenyl(tetramethylrhodamine 2,3-diaminopropionate-O,N)borane-   diphenyl(tetramethylrhodamine 2,6-diaminocapronate-O,N)borane-   diphenyl(FITC-2,6-diaminocapronate-O,N)borane-   diphenyl(2,3-diaminobutyrate-O,N)borane-   diphenyl(2,5-diaminopentanate-O,N)borane-   di(3-chloro-4-methylphenyl)(anthranate-O,N)borane-   di(trifluoromethylphenyl) 2-aminoethylborinate-   di(3-chloro-4-methylphenyl)(glutaminate-O,N)borane-   dibutyl(asparagine-O,N)borane-   di(4-(phenyl-2-pyridylmethoxyboryl)benzyl)ether-   di(1-(pyridin-2-yl)-1-(4-methoxyphenyl)methyl-phenyl-borylbenzyl)ether-   bis((4,4′-phenylhydroxyboryl)benzyloxybenzyl)hydroxyborane-   di(trifluoromethylphenyl) 2-propylaminoethylborinate-   bis((4,4′-phenylaminoethoxyboryl)benzyloxybenzyl)aminoethoxyborane-   bis((4,4′-phenyl    methylaminoethoxyboryl)benzyloxybenzyl)methylaminoethoxyborane-   bis((4,4′-phenyldimethylaminoethoxyboryl)benzyloxybenzyl)dimethylaminoethoxyborane-   bis((4,4′-phenyl    2-pyridyl-4-trifluoromethylphenylmethoxyboryl)benzyloxybenzyl)    2-pyridyl-4-trifluoromethyl phenylmethoxyborane-   diphenyl(2-piperazine-3-carboxyamide-carboxy)borane-   diphenyl(methionate-O,N)borane-   phenyl 3-piperidinooxyboryl phenylether-   4,4′-(phenyl piperazino-O,O-ethoxyboryl)phenylether-   4,4′-(phenyl piperazino-O,O-ethoxyboryl)benzylether-   bis(4,4′-(phenyldimethylaminoethoxyboryl)phenyl)ether-   bis(3,3′-(phenylbenzylaminoethoxyboryl)phenyl)ether-   di(3-chloro-2-methylphenyl)borinic acid-   4,4′-di((3-chloro-4-methylphenyl 2-hydroxyboryl)phenyl)ether-   phenyl naphthyl 2-pyridylmethylborinate-   phenyl naphthyl dimethylaminoethylborinate-   phenyl naphthyl benzylaminoethylborinate-   bis(4,4′-(phenyl 2-amino-2-benzylethoxyboryl)benzyl)ether-   bis(3,3′-(phenyldimethylaminoethoxyboryl)benzyl)ether-   di(3-chloro-4-methylphenyl)dimethylaminoethylborinate-   di(3-chloro-4-methylphenyl)-2-benzyl-2-aminoethylborinate-   di(3-chloro-4-methylphenyl)1-phenyl 2-aminoethylborinate-   di(3-chloro-4-methylphenyl)butylaminoethyl borinate-   di(3-chloro-4-methylphenyl)benzylaminoethyl borinate-   diphenyl(R) 2-benzyl-2-aminoethyl borinate-   diphenyl(S) 2-benzyl-2-aminoethyl borinate-   di(3-chloro-4-methylphenyl) 1-phenylaminoethylborinate-   di(3-chloro-4-methylphenyl)pyridylmethylborinate-   di(3-chloro-4-methylphenyl)borinic acid anhydride-   diphenylborinic acid anhydride-   diphenyl(picolinate-O,N)borane-   diphenyl(2-aminophenyl carboxylate-O,N)borane-   di(3-chloro-4-methylphenyl) 2-aminophenylborinate-   di(3-chloro-4-methylphenyl)(2-pyridine carboxylate-O,N)borane-   poly(4,4′-diphenylether glutamine-O,N)borane-   poly(4,4′-diphenyl glutamine-O,N borane)-   diphenyl 1-(2-aminobenzyl) 1-phenylmethylborinate-   di(3-chloro-4-methylphenyl) 1-(2-aminobenzyl) 1-phenylmethylborinate-   diphenyl(2-aminohexanecarboxylate-O,N)borane-   di(3-chloro-4-methylphenyl)(norloysinate-O,N)borane-   diphenyl 2-aminobutylborinate-   di(3-chloro-4-methylphenyl) 2-aminobutylborinate-   di(trifluoromethylphenyl)borinic acid-   di(3-chloro-4-methylphenyl)borinic acid-   di(trifluoromethylphenyl) 2-aminoethylborinate-   di(trifluoromethylphenyl) 2-dimethylaminoethylborinate-   di(4-chloro-3-fluoro-phenyl) 2-aminoethylborinate-   di(4-chloro-2-fluorophenyl) 2,3-diamino-2-propyl-borinate-   di(4-chloro-3-fluorophenyl) 2-amino-2-methyl-propyl-borinate-   di(4-chloro-3-fluorophenyl) 2-phenylaminoethyl borinate-   di(4-chloro-3-fluorophenyl) 2-amino-3-hydroxybutyl borinate-   bis(diphenyl piperazino-O,O-ethoxyborane)-   4-((2-aminoethoxy)phenylboryl)benzyl-4′-((2-aminoethoxy)phenylboryl)phenethylether-   di(3-chlorophenyl)borinic acid-   di(5-chloro-2-methylphenyl) 2-piperidinomethylborinate-   di((5-chloro-2-methylphenyl)hydroxyborylphenyl)ether-   di(5-chloro-2-methylphenyl) 2-aminoethylborinate-   diphenyl(ornithine-O,N)borane-   di(5-chloro-2-methylphenyl) 2-butylaminoethylborinate-   di(3-chloro-4-methylphenyl) 2-piperidinomethylborinate-   di(3-chloro-4-methylphenyl) 2-piperidinoethylborinate-   4,4′-((2-aminoethoxy)(3-chloro-4-methylphenyl)boryl)diphenylether-   bis(4,4′-(phenyldimethylaminoethoxyboryl)phenyl)ether-   bis(3-chloro-4-methylphenyl hydroxyborylphenyl)ether-   1,4-bis(phenylhydroxyboryl)benzene-   di(2-thiophene)borinic acid-   diphenyl(glycinate-O,N)borane-   diphenyl(serinate-O,N)borane-   diphenyl(glutaminate-O,N)borane-   diphenyl(asparaginate-O,N)borane-   diphenyl(alaninate-O,N)borane-   diphenyl(phenylalaninate-O,N)borane-   diphenyl(tryptophanate-O,N)borane-   diphenyl(leucinate-O,N)borane-   diphenyl(isoleucinate-O,N)borane-   diphenyl(2,4-diaminolactonate-O,N)borane-   diphenyl(tyrosinate-O,N)borane-   diphenyl(threoninate-O,N)borane-   diphenyl(cysteinate-O,N)borane-   diphenyl(histidinate-O,N)borane-   diphenyl(hydroxyprolinate-O,N)borane-   diphenyl(glutaminate-O,N)borane-   diphenyl(asparaginate-O,N)borane-   diphenyl(lysinate-O,N)borane-   diphenyl(2,3-diaminopropionate-O,N)borane-   bis(4,4′-(phenyl-glutamineboryl)phenyl)ether-   bis(4,4′-(phenylasparagineboryl)phenyl)ether-   (4-(phenyl-glutamic acid    boryl)phenyl)-(4′-(hydroxymethylphenyl-glutamic acid    boryl)phenyl)ether-   diphenyl(glutaminate-O,N)borane-   diphenyl(prolinate-O,N)borane-   (3-phenoxybenzyl)-(3′-(phenyl-2-aminoethoxyboryl)benzyl)ether-   diphenyl(2-piperazinecarboxy)borane-   diphenyl(2,4-diaminolacetic acid)borane-   di(3-chloro-4-methylphenyl)-(picolinate-O,N)borane-   di(3-chloro-4-methylphenyl)(asparaginate-O,N)borane-   di(3-chloro-4-methylphenyl) 2-aminophenylthioborane-   di(4-trifluoromethylphenyl)(picolinate-O,N)borane-   di(4-trifluoromethylphenyl) 2-aminoethylthioborane-   di(3-chloro-4-methylphenyl)(2,6-diaminopimelinate-O,N)borane-   di(3-chloro-4-methylphenyl)(citrullinate-O,N)borane-   di(3-chloro-4-methylphenyl)(glycylglutaminate-O,N)borane-   di(4-trifluoromethylphenyl)(1,3-propylenediaminediacetate-O,N)borane-   di(4-trifluoromethylphenyl)(glycylglycinate-O,N)borane-   di(3-chloro-4-methylphenyl)(allothreoninate-O,N)borane-   di(3-chloro-4-methylphenyl)(norloysinate-O,N)borane-   di(3-chloro-4-methylphenyl)(2,4-diaminobutyrate-O,N)boranediphenyl    dimethylaminoethylthioborane-   di(3-chloro-4-methylphenyl)dimethylaminoethylthioborane-   (4-(2-thiophenehydroxyboryl)phenoxyethyl)(4′-(2-thiophenehydroxyboryl)benzyl)ether-   1,2-di(phenylhydroxyboryl)benzene-   1,2-di(phenylaminoethoxyboryl)benzene-   poly(2,5-dimethylphenyl asparagine-O,N borane)-   poly(phenylene 2-aminoethylaminoethoxy borane)-   poly(phenylene 2-pyridylmethoxy borane)-   poly(1,4-phenylenehydroxyboryl-1,3-phenyleneborinic acid)-   poly(1,4-phenylene aminoethoxyboryl-1,3-phenyleneaminoethoxyborane)-   2,8-di(3-thiophenylglutamine-O,N boryl)dibenzothiophene-   4,4′-(dicyano-phenyl)borinic acid-   3,3′-(dicyano-phenyl)borinic acid-   diphenyl(citrullinate-O,N)borane-   diphenyl(ornithinate-O,N)borane-   poly(1,2-phenylene-hydroxyborane)-   poly(2,5-dimethyl-1,4-phenylene-hydroxyborane)-   poly(2-methyl-1,3-phenylene-hydroxyborane)-   poly(2,8-dibenzothiophenylene-hydroxyborane)-   poly(2,2′-biphenylene-hydroxyborane)-   poly(1,4-naphthalene-hydroxyborane)-   poly(9,10-anthracene-hydroxyborane)-   poly(3,6-carbazole-hydroxyborane)-   poly(5-methyl-1,3-phenylene-hydroxyborane)-   poly(5,5′-bithiophene-hydroxyborane)-   poly(2,2′-binaphthyl-hydroxyborane)-   poly(4,4′-biphenylene aminoethoxyborane)-   poly(4,4′-biphenylene N-hydroxyethylaminoethoxyborane)-   bis(4,4′-(3-chloro-4-methylphenylhydroxyboryl)benzyl)ether-   poly(4-phenylborinic acid)-   naphthaleneboronic acid-   bis(4-(4-trifluoromethylphenylhydroxyboryl)benzyl)ether-   poly(2,5-dimethylphenyl aminopropoxyborane)-   poly(2,5-dimethylphenyl aminopropylthioborane)-   bis(3-(4-methoxyphenylhydroxyboryl)benzyl)ether-   (3-(phenylhydroxyboryl)benzyl) (4-(phenylhydroxyboryl)benzyl)ether-   (2-(phenylhydroxyboryl)benzyl) (3-(phenylhydroxyboryl)benzyl)ether-   (2-(phenylhydroxyboryl)benzyl) (4-(phenylhydroxyboryl)benzyl)ether-   (3-(phenylaminoethoxyboryl)benzyl)    (4-(phenylaminoethoxyboryl)benzyl)ether-   bis(3-(3-chloro-4-methylphenylhydroxyboryl)benzyl)ether-   (2-(phenylaminoethoxyboryl)benzyl)    (3-(phenylaminoethoxyboryl)benzyl)ether-   (2-(phenylaminoethoxyboryl)benzyl)    (4-(phenylaminoethoxyboryl)benzyl)ether-   bis(3-(4-fluorophenylhydroxyboryl)benzyl)ether-   bis(3-(4-fluorophenylaminoethoxyboryl)benzyl)ether-   bis(4-(4-chloro-3-methyl-phenyl)hydroxyborylbenzyl)ether-   bis(4-(4-chloro-3-methyl-phenylaminoethoxyborylbenzyl)ether-   bis(3-(3′,4′-methylenedioxy-phenylhydroxyboryl)benzyl)ether-   (3-(3-chloro-4-methylphenylhydroxyboryl)benzyl)    (4-(3-chloro-4-methylphenylhydroxyboryl)benzyl)ether-   (3-(3′,4′,5′-trifluorophenylhydroxyboryl)benzyl)(4-(3′,4′,5′-trifluorophenylhydroxyboryl)benzyl)ether-   bis(3-(4-methoxyphenylaminoethoxyboryl)benzyl)ether-   (3-(4-chloro-3-methylphenylhydroxyboryl)benzyl)(2-(4-chloro-3-methylphenylhydroxyboryl)benzyl)ether-   bis(3-(4-cyanophenylhydroxyboryl)benzyl)ether-   bis(3-(2′-thiophenylhydroxyboryl)benzyl)ether-   bis(3-(1′-naphthylhydroxyboryl)benzyl)ether-   bis(4-(2-methoxy-5-fluorophenylhydroxyboryl)benzyl)ether-   bis(4-(2-methoxy-5-fluorophenylaminoethoxyboryl)benzyl)ether-   (3-(4-chloro-3-methyl-phenylaminoethoxyboryl)benzyl)(2-(4-chloro-3-methyl-phenylaminoethoxyboryl)benzyl)ether-   bis(4-(3,4-difluorophenylhydroxyboryl)benzyl)ether-   bis(4-(3,4-difluorophenylaminoethoxyboryl)benzyl)ether-   (3-(3′,4′,5′-trifluorophenylaminoethoxyboryl)benzyl)(4-(3′,4′,5′-trifluorophenylaminoethoxyboryl)benzyl)ether-   5,5′-(phenylhydroxyboryl)-2,2′-dithiophene-   5,5′-(phenylaminoethoxyboryl)-2,2′-dithiophene-   3,5-di(phenylaminoethoxyboryl)toluene-   2,5-di(phenylhydroxyboryl)toluene-   2,2′di(phenylhydroxyboryl)-1,1′-binaphthyl-   2,2′-di(phenylaminoethoxyboryl)-1,1′-binaphthyl-   bis(4-(4-methylphenylhydroxyboryl)benzyl)ether-   bis(4-(4-methylphenylaminoethoxyboryl)benzyl)ether-   4,4′-(4-methylphenylhydroxyboryl)diphenyl-   4,4′-(4-methylphenylaminoethoxyboryl)diphenyl-   4,4′-(4-methylphenylhydroxyboryl)diphenylether-   poly(2,5-dimethylphenyl 2-pyridylmethoxyborane)-   4,4′-bis(3-chloro-4-methyl-phenylhydroxyboryl)diphenylether-   (2-(phenylhydroxyboryl)phenethyl)((2-phenylhydroxyboryl)benzyl)ether-   (2-(phenylaminoethoxyboryl)phenethyl)((2-phenylaminoethoxyboryl)benzyl)ether-   (4-phenylhydroxyborylphenyl)(4′-phenylhydroxyborylbenzyl)ether-   (4-phenylaminoethoxyborylphenyl)(4′-phenylaminoethoxyborylbenzyl)ether-   (4-trifluoromethylphenylhydroxyborylphenyl)(4′-trifluoromethylphenylhydroxyborylbenzyl)ether-   (4-trifluoromethylphenylaminoethoxyborylphenyl)(4′-trifluoromethylphenylaminoethoxyborylbenzyl)ether-   9,10-bis-(trifluoromethylphenylhydroxyboryl)anthracene-   9,10-bis-(trifluoromethylphenylaminoethoxyboryl)anthracene-   bis(3-(1-naphthylaminoethoxyboryl)benzyl)ether-   4,5-di(phenylhydroxyboryl)-2,7-di-tert-butyl-9,9-dimethylxanthrene-   4,5-di(phenylaminoethoxyboryl)-2,7-di-tert-butyl-9,9-dimethylxanthrene-   (4-(phenylhydroxyboryl)phenoxyethyl)(4-(phenylhydroxyboryl)benzyl)ether-   (4-(phenylaminoethoxyboryl)phenoxyethyl)(4-(phenylaminoethoxyboryl)benzyl)ether-   6,6′-(phenylhydroxyboryl)-2,2′-dipyridyl-   6,6′-(phenylaminoethoxyboryl)-2,2′-dipyridyl-   bis(2,5-(phenylhydroxyboryl))furan-   bis(2,5-(phenylaminoethoxyboryl))furan-   bis(4,4′-(phenyl-N,N-dimethylaminoethoxyboryl)phenyl)ether-   bis(4,4′-(phenyl-N-methylaminoethoxyboryl)phenyl)ether-   2,8-di(phenylhydroxyboryl)dibenzothiophene-   bis(4,4′-(phenyl-glutamineboryl)phenyl)ether-   2,8-di(3-thiophenyl-2-pyrrolidinomethoxyboryl)dibenzothiophene-   bis(4,4′-(phenyl-asparagineboryl)phenyl)ether-   (4-(phenyl-N-methylaminoethoxyboryl)phenyl)(4′-(hydroxymethylphenyl-N-methylaminoethoxyboryl)phenyl)ether-   (4-(phenyl-N,N-dimethylaminoethoxyboryl)phenyl)(4′-(hydroxymethylphenyl-N,N-dimethylaminoethoxyboryl)phenyl)ether-   (4-(phenyl-glutamic acid    boryl)phenyl)(4′-(hydroxymethylphenyl-glutamic acid    boryl)phenyl)ether-   (4-(phenyl-glutamineboryl)phenyl)(4′-(hydroxymethylphenyl-glutamineboryl)phenyl)ether-   bis(4,4′-(phenyl-N,N-dimethylaminoethoxyboryl)phenyl)ether-   bis(4,4′-(phenyl-N-aminoethyl-aminoethoxyboryl)phenyl)ether-   (4-(phenyl-cysteineboryl)phenyl)(4′-(hydroxymethylphenyl-cysteineboryl)phenyl)ether-   bis(4,4′-(phenoxyphenyl-aminoethoxyboryl)phenyl)ether-   bis(4,4′-(phenyl-N-aminoethyl-aminoethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-N-methylaminoethoxyboryl)benzyl)ether-   (4′-trifluoromethylphenyl-N,N-dimethylaminoethoxyboryl)-4-phenyl(4′-trifluoromethylphenyl-N,N-dimethylaminoethoxyborylbenzyl)ether-   (4′-trifluoromethylphenyl-N-methylaminoethoxyboryl)-4-phenyl    (4′-trifluoromethylphenyl-N-methylaminoethoxyboryl-4-benzyl)ether-   bis(3,3′-(phenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-asparagineboryl)benzyl)ether-   bis(3,3′-(phenyl-aminoethylthioboryl)benzyl)ether-   2,8-di(3-thiophenylhydroxyboryl)dibenzothiophene-   bis(4,4′-(p-trifluoromethylphenyl-hydroxyboryl)benzyl)ether-   2,8-di(phenylaminoethoxyboryl)dibenzothiophene-   bis(4,4′-(phenyl-lysineboryl)benzyl)ether-   bis(4,4′-(p-methoxy-phenyl-hydroxyboryl)benzyl)ether-   bis(4,4′-(3,4-difluorophenyl-hydroxyboryl)benzyl)ether-   bis(4,4′-(p-methoxyphenyl-aminoethoxyboryl)benzyl)ether-   bis(4,4′-(p-methoxyphenyl-N-methylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(p-methoxyphenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(p-methoxyphenyl-2,4-diaminobutyric acid boryl)benzyl)ether-   bis(4,4′-(3,4-difluorophenyl-aminoethoxyboryl)benzyl)ether-   bis(4,4′-(3,4-difluorophenyl-N-methylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(3,4-difluorophenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(3,4-difluorophenyl-N-aminoethylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(3-chloro-4-methylphenyl-aminoethoxyboryl)benzyl)ether-   bis(4,4′-(3-chloro-4-methylphenyl-N-methylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(3-chloro-4-methylphenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(3-chloro-4-methylphenyl-2-piperidylmethoxyboryl)benzyl)ether-   bis(4,4′-(p-trifluoromethylphenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(p-trifluoromethylphenyl-asparagineboryl)benzyl)ether-   bis(4,4′-(p-trifluoromethylphenyl-aminoethoxyboryl)benzyl)ether-   (4-phenyl-N-methylaminoethoxyborylphenyl)(4′-phenyl-N-methylaminoethoxyborylbenzyl)ether-   (4-phenyl-N,N-dimethylaminoethoxyborylphenyl)    (4′-phenyl-N,N-dimethylaminoethoxyborylbenzyl)ether-   (4-phenyl-2-pyridylmethoxyborylphenyl)(4′-phenyl-2-pyridylmethoxyborylbenzyl)ether-   4-(phenyl-p-methoxyphenyl-2-pyridylmethoxyboryl)-phenyl    4′-(phenyl-p-methoxyphenyl-2-pyridylmethoxyboryl)benzylether-   bis(4,4′-(phenyl-3-piperidyloxyboryl)phenyl)ether-   bis(4,4′-(phenyl-2-pyridylmethoxyboryl)phenyl)ether-   bis(4,4′-(phenyl-aminoethylthioboryl)phenyl)ether-   bis(4,4′-(phenyl-2-amino-1-phenylethoxyboryl)phenyl)ether-   bis(4,4′-(phenyl-ornithineboryl)phenyl)ether-   bis(4,4′-(phenyl-2,3-diaminopropionic acid boryl)phenyl)ether-   bis(4,4′-(phenyl-lysineboryl)phenyl)ether-   bis(4,4′-(phenyl-2-pyrrolidinemethoxyboryl)phenyl)ether-   bis(4,4′-(naphthylhydroxyboryl)phenyl)ether-   bis(4,4′-(tolylhydroxyboryl)phenyl)ether-   bis(4,4′-(naphthyl-aminoethoxyboryl)phenyl)ether-   bis(4,4′-(naphthyldimethylaminoethoxyboryl)phenyl)ether-   bis(4,4′-(naphthyl-2-pyridylmethoxyboryl)phenyl)ether-   bis(4,4′-(naphthylglutamineboryl)phenyl)ether-   bis(4,4′-(naphthyl 2,4-diaminopropionic acid boryl)phenyl)ether-   bis(4,4′-(tolyldimethylaminoethoxyboryl)phenyl)ether-   bis(4,4′-(tolylpiperadylethoxyboryl)phenyl)ether-   bis(4,4′-(tolylasparagineboryl)benzyl)ether-   bis(4,4′-(tolyllysineboryl)phenyl)ether-   bis(4,4′-(phenyl-aminoethylthioboryl)benzyl)ether-   bis(4,4′-(phenyl-2-pyrrolidinemethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-2,4-diaminobutyric acid boryl)benzyl)ether-   bis(4,4′-(phenyl-butylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-phenylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-benzylaminoethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-N-methylpiperidine-methoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-1-methyl-2-aminoethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-1-piperidylethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-2-pyrrolidinomethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-2-phenyl-2-aminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-2-piperidylmethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-dimethylaminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-1-methyl-2-aminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-1-piperidylethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-2-pyridylmethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-2-amino-1-phenylethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-N-methylaminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-N-aminoethyl-1-methyl-2-aminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-glutamineboryl)benzyl)ether-   bis(3,3′-(phenyl-2,4-diaminobutyric acid boryl)benzyl)ether-   bis(3,3′-(phenyl-N-butylaminoethoxyboryl)benzyl)ether-   bis(3,3′-(phenyl-asparagineboryl)benzyl)ether-   bis(3,3′-(phenyl-lysineboryl)benzyl)ether-   bis(3,3′-(phenyl-ornithineboryl)benzyl)ether-   bis(4,4′-(phenyl-2-methyl-8-quinolinooxyboryl)phenyl)ether-   bis(4,4′-(phenyl-2-benzyl-2-amino-ethoxyboryl)benzyl)ether-   bis(4,4′-(phenyl-2-benzyl-2-amino-ethoxyboryl)phenyl)ether-   bis(3,3′-(phenyl-2-benzyl-2-amino-ethoxyboryl)benzyl)ether-   2,8-di(phenylglutamine-O,N borane)dibenzothiophene-   2,8-di(phenyl 2-pyrrolidinomethoxyboryl)dibenzothiophene-   2,8-di(phenylarginine-O,N borane)dibenzothiophene-   2,8-di(3-thiophenylaminoethoxyboryl)dibenzothiophene-   bis(2,2′-(phenylhydroxyboryl)benzyl)ether-   2-aminoethyl diphenylborinate-   diphenylborinic acid-   poly(4,4′-biphenylene aminoethylthioborane)-   poly(4-phenylborinic acid)-   poly(dimethylaminoethoxyphenyleneborane)-   1,3,5-tri(phenyl 2-aminoethoxyboryl)benzene-   dibutyl(phenylalanine-O,N)borane-   4,4′-di(phenyl    1-(pyridin-2-yl)-1-trifluoromethylphenylmethoxyboryl)benzylether-   di(3-chloro-6-methylphenyl)aminoethylborinate-   bis(4,4′-(p-trifluoromethylphenyl-asparagineboryl)benzyl)ether-   di(3-chloro-4-methyl)phenyl(methionate-O,N)borane-   poly(1,4-phenylene 2-pyridylmethoxyborane)-   poly(diphenyletherhydroxyborane)-   4,4′-di(phenyl    1-(pyridin-2-yl)-1-trifluoromethylphenylmethoxyborylbenzyl)ether

The present invention also relates to the compounds represented by thefollowing formula (4′) or (8′) or a pharmaceutically acceptable saltthereof.R₃′—[—X′—B(ZR₁′)—]_(n)—R₄′  (4′)R₃′—B(ZR₁′)—X′—B(ZR₂′)—R₄′  (8′)

In the formulas, B is a boron atom, Z is O or S, R₁′ and R₂′ are H,—(CH₂)_(m)—NR₅′R₆′, —CHR₁₁′R₁₂′, —COCH(NH₂)—(CH₂)_(m)NHCONH₂ or—COCH(NH₂)—(CH₂)_(m)—COR₁₉′. Here, R₅′, R₆′, R₁₁′, R₁₂′ and R₁₉′ areindependently H, or amino or heterocyclyl, each of which is substitutedor unsubstituted. R₃′ and R₄′ are H, aryl or heterocyclyl, X′ issubstituted or unsubstituted aromatic group, m is an integer of 1-5, andn is an integer of 1-100.

The “amino”, “heterocyclyl”, “aryl” and “aromatic group” are as definedabove.

Specifically, the following compound can be mentioned:

The compounds (1)-(13) in the present invention can be converted topharmaceutically acceptable non-toxic salts by a known method. Thenon-toxic salts include, for example, alkali metal salts, alkaline earthmetal salts, amine salts, acid addition salts, solvates (includinghydrates) and the like. In general, water-soluble ones are preferable.

Suitable non-toxic salts are salts with alkali metal such as potassium,sodium and the like; salts with alkaline earth metal such as calcium,magnesium and the like; and salts with organic amine such astriethylamine, methylamine, dimethylamine, cyclopentylamine,benzylamine, phenethylamine, piperidine, monoethanolamine,diethanolamine, tris(hydroxymethyl)aminomethane, lysine, arginine,N-methyl-D-glucamine and the like, preferably, alkali metal salts.

Moreover, as suitable acid addition salts, inorganic acid salts such ashydrochloride, hydrobromide, sulfate, phosphate, nitrate, and organicacid salts such as acetate, trifluoroacetate, lactate, tartrate,oxalate, fumarate, maleate, citrate, benzoate, methanesulfonate,ethanesulfonate, benzenesulfonate, toluenesulfonate, isethionate,glucuronate and gluconate can be mentioned.

The compound of the present invention also includes solvates. Solvate isa conjugate, particularly in a crystal form, of the aforementionedcompound of the present invention and a pharmaceutically acceptablesolvent (for example, water, organic solvent) at a stoichiometrical ornon-stoichiometrical ratio.

The present invention relates to a prophylactic and/or therapeutic drugfor a disease caused by protein cross-linking, which contains theaforementioned protein cross-linking inhibitor.

As the disease caused by abnormal protein cross-linking, for example,Alzheimer's disease, Huntington's disease, Parkinson's disease, Celiacdisease, cataract, mad cow disease, congenital lamellar ichthyosis andcongenital hemostatic disorder can be mentioned. Particularly, it isdesirably used as prophylactic and/or therapeutic drug for Alzheimer'sdisease.

The compound of the present invention can be synthesized by the methodsdescribed in WO03/033002 and WO2007/061074 or a method analogousthereto. In addition, the compound of the present invention can besynthesized by the following method or a method analogous thereto.

The main starting materials for the production of the compound of thepresent invention are monobromo compound, dibromo compound andalkoxyborane. A bromo compound is reacted with alkyl lithium to give alithium compound R₃—Li (formula (17)). A dibromo compound (Br—X—Br orBr—Y—Br) is reacted with alkyl lithium to give a dilithium compound(Li—X—Li or Li—Y—Li) (formula (14) and formula (15)). Alternatively,magnesium is reacted to give a Grignard reagent (formula (16)). Thesemetal compounds are reacted with trialkoxyborane to give dialkoxyboraneR₃—B(OAlk)₂ (formula (19)). R₃—B(OAlk)₂ is reacted with Li—X—Li to giveR₃—B(OAlk)-X—B—(OAlk)-R₃ (formula (20)) (Alk is an alkyl group having 1to 4 carbon atoms). A dilithium compound (Li—X—Li) is reacted withR₃—B(OAlk)-X—B—(OAlk)-R₃ to give (—B(OAlk)-X—)_(n). The resultantproduct is treated with acidic water to give (—B(OH)—X—), (formula(22)). R₃—Li, R₄—Li, (OAlk)₂B—X—B(OAlk)₂ and Li—Y—Li are reacted to giveR₃—(—Y—B(OAlk)-X—B(OAlk)-)_(n)—R₄ and this is treated with acidic waterto give R₃—(—Y—B(OH)—X—B(OH)—)_(n)—R₄ (formula (23)). Li—X—Li is reactedwith (OAlk)₂B—Y—B(OAlk)₂ to give (—X—B(OAlk)-Y—B(OAlk-)_(n), which istreated with acidic water to give (—X—B(OH)—Y—B(OH)—)_(n) (formula(24)). These two bifunctional compounds are reacted to give variousborinic acids. Borinic acid is reacted with desired HZR wherein R is R₁or R₂ used in the formulas (1)-(13)) to give the object compound(formulas (25) and (26)).

By a reaction with diphenylborinic acid using amino acid and βaminothiol instead of β amino alcohol, a dehydrating reaction occurs anda desired compound can be obtained (formula (27), formula (28)).C₆H₅B(OH)C₆H₅+HOOC₆CHRNH₂→C₆H₅B(OCOCHRNH₂)C₆H₅  (27)C₆H₅B(OH)C₆H₅+HSCH₂CH₂NH₂→C₆H₅B(SCH₂CH₂NH₂)C₆H₅  (28)

As for a part of the compounds (1)-(13) of the present invention,according to the aforementioned schemes (14)-(26), borinic acid issynthesized from a bromine compound and bromobenzene by a similarmethod, which is reacted with amino alcohol, amino acid or aminothiol tosynthesize a desired compound.

In addition, a compound represented by the formula (4′) or (8′) can besynthesized according to the formulas (20), (29), (30) and (31).

In the following, the compounds represented by the formulas (1)-(13)(including compounds represented by the formulas (4′) and (8′)) are alsoreferred to as the compound of the present invention.

In the present invention, the enzyme (transglutaminase (TGase))inhibitory action is determined by measuring the enzyme activity by anappropriately-modified method based on Lorand et al. (Lorand, L. et al.(1971), Anal Biochem. 1971 November; 44(1):221-31.). For example, themethod described in the Example can be performed.

In the present invention, the polyglutamine aggregation inhibitoryactivity (x-Fold) can be measured, for example, by the method describedin the Example.

The SOC (store operated calcium channel)-suppressive action can bemeasured by the method described in the Example and using, for example,FDSS 3000.

The compound of the present invention (i.e., active substance or activeingredient) is administered systemically or topically in an oral orparenteral dosage form to a test subject (mammal inclusive of human,preferably human). The parenteral administration includes intravenousadministration, intraarterial administration, intramuscularadministration, subcutaneous administration, intradermal administration,intraperitoneal administration, intrarectal administration, intraduraladministration, vaginal administration, transmucosal administration andthe like.

While the dose varies depending on the kind of the compound to beadministered, age, body weight and symptom of the subject ofadministration, treatment effect, administration method and the like,generally, for example, 10 μg-1000 mg is orally administered to oneadult (body weight about 60 kg) once to several times per day or, forexample, 1 μg-100 mg is parenterally administered to one adult (bodyweight about 60 kg) once to several times per day.

The administration preparation of the compound of the present inventionincludes, but are not limited to, tablet, pill, suspension, solution,capsule, syrup, elixir, granule, powder and the like for oraladministration, injection, external preparation, suppository, externalliquid, ointment, embrocation, inhalant, spray, pessary for vaginaladministration and the like for parenteral administration.

The aforementioned preparation can contain a pharmacologicallyacceptable carrier (excipient, diluent and the like) or an additive incombination with the compound of the present invention as an activeingredient.

As the aforementioned excipient and additive, those conventionally usedin the field of medicaments can be used. For example, the agents andformulation methods described in Remington: The Science and Practice ofPharmacy 9^(th) ed. (1995) MACK PUBLISHING COMPANY (US) can be referredto.

Examples of the excipient include lactose, mannitol, glucose,microcrystalline cellulose, starch and the like.

Examples of the additive include binders (hydroxypropylcellulose,polyvinylpyrrolidone, magnesium alumino metasilicate etc.),disintegrants (calcium cellulose glycolate etc.), lubricants (magnesiumstearate etc.), stabilizers, solubilizing agents (glutamic acid,aspartic acid etc.) and the like.

The preparation of the present invention may be coated with a coatingagent (sucrose, gelatin, hydroxypropylcellulose,hydroxypropylmethylcellulosephthalate etc.), or may be coated with twoor more layers. By applying such coating, the forms of control releasepreparation, enteric preparation and the like can be provided. Further,a capsule of absorbable substances such as gelatin is also encompassed.

In a liquid for oral administration, one or more of the activitysubstances are dissolved, suspended or emulsified in a generally-useddiluent (purified water, ethanol, buffer, or a mixed solution thereofetc.). Further, the liquid may contain a wetting agent, a suspendingagent, an emulsifier, a stabilizer, a sweetening agent, a flavoringagent, an aromatic, a preservative, a buffering agent and the like.

The injection for parenteral administration includes a solution, asuspension, an emulsion and an injection obtained by dissolving orsuspending in a solvent when in use. An injection can be obtained bydissolving, suspending or emulsifying one or more active substances in asolvent. As the solvent, for example, distilled water for injection,saline, vegetable oil, alcohols such as propylene glycol, polyethyleneglycol and ethanol and a combination thereof are used. Furthermore, theinjection may contain a stabilizer (amino acid such as lysine,methionine and the like, sugar such as trehalose and the like), asolubilizing agent (glutamic acid, aspartic acid, polysorbate 80(registered trademark) etc.), a suspending agent, an emulsifier, asoothing agent, a buffering agent, a preservative and the like. Theseinjections are sterilized in the final step or produced and prepared byan aseptic operation method. In addition, an aseptic solid agent, forexample, a freeze-dried product may be produced, and dissolved insterilized or aseptic distilled water for injection or other solvent andused.

A spray may contain, besides a generally-used diluent, a stabilizer suchas sodium bisulfite and a buffering agent that achieves isotonicity, forexample, an isotonic agent such as sodium chloride, sodium citrate andcitric acid.

In the present specification, when the terminal group is a hydroxylgroup, a chemical formula omitting a hydroxyl group is sometimesdescribed. The number after the compound name is the compound No.

EXAMPLE Experimental Example 1 Measurement of TG

The compound of the present invention (10 mM, 1 μL) was taken in a96-well plate (Nunc, 96 Well Black Plate with Clear Bottom), an enzymereaction solution (100 mM HEPES-NaOH, pH 7.5, 1 mM CaCl₂, 20 μMmonodansyl cadaverine, 0.05 mg/mL N,N-dimethylcasein, 5 μg/mL TGase)(0.1 ml) was added and the mixture was sufficiently mixed without makingfoams. The mixture was set on a fluorescence drug screening system FDSS3000 (Hamamatsu Photonics K.K.), and changes in the fluorescencewavelength per unit time at 340 nm were measured, based on which theTGase inhibitory activity of the compound of the present invention wascalculated. As a control, change in the fluorescence when 1 μL of DMSO(dimethyl sulfoxide) was added instead of the compound of the presentinvention was taken as 100, and TG50 was when the activity decreased tohalf due to the compound of the present invention. The results are shownin the following.

Experimental Example 2 Measurement of x-Fold

Truncated N-terminal huntington 150 Q-EGFP-Neuron 2a cells (preparedaccording to Wang, G. H., Nukina, N et al, Neuroreport, 10, 2435-2438(1999)) were cultured for one day in a 96-well plate, 1 μM ponasterone A(2 μL) and 5 μM dibutyl cyclic AMP (2 μL) were added such that theconcentration of the compound of the present invention became 20 μM, andthe mixture was cultured for 20 hr. The cells were fixed with 4%para-formaldehyde and, 30 min later, the cells were washed with PBS andstained with Hoechst 33342. The number of the aggregated cells, and thetotal number of cells were counted by Array Scan V T1 (manufactured byCellomics, Pittuburg, USA), and the ratio of the aggregated cells to thetotal number of cells was determined (x-Fold). Without the compound ofthe present invention, the respective numbers of cells were almost thesame, and the number of the aggregated cells to the total number ofcells was almost 1. A smaller value shows a stronger polyglutamineaggregation inhibitory activity. The results are shown in the following.

Experimental Example 3 Measurement of SOC IC50

CHO cell culture medium was replaced with a BSS solution which is anextracellular fluidfree of calcium, the compound of the presentinvention was added 1 min later, and 1 μM thapsigargin was allowed toact thereon 2 min later to deplete intracellular calcium store. After 9min, to the extracellular fluid was added calcium chloride at the finalconcentration of 2 mM, and an influence of each compound on the degreeof increase in the intracellular calcium concentration after additionwas estimated, based on which SOC suppressive action (IC50) wasdetermined. The results are shown in the following.

Example 1 2-aminoethylthio bis(4-chloro-2-fluorophenyl)borane (6014)

TG 28, x-Fold 0.95

Example 2(4-(phenylglutamineboryl)phenyl)(4′-(phenylhydroxyboryl)phenyl)ether(7111)

TG 28, x-Fold 0.82, SOC IC50 0.2 μM

Example 3 bis(4,4′-(phenylhydroxyboryl)phenyl)ether (536)

TG −20, x-Fold 0.49, SOC IC50 0.5 μM

Example 4 poly(4,4′-biphenylene N-methylaminoethoxyborane) (1130)

TG 109, x-Fold 0.80, SOC IC50 5 μM

Example 5 bis(4,4′-(phenylaminoethoxyboryl)phenyl)ether (1022)

TG −4, x-Fold 0.60, SOC IC50 0.15 μM

Example 6(4-(phenylasparagineboryl)phenyl)(4′-(phenylhydroxyboryl)phenyl)ether(7132)

TG 23, x-Fold 1.01, SOC IC50 0.2 μM

Example 7 bis(3,3′-(phenylhydroxyboryl)benzyl)ether (162OH)

TG 14, x-Fold 1.03, SOC IC50 0.2 μM

Example 8 bis(3,3′-(phenylaminoethoxyboryl)benzyl)ether (162AE)

TG 24, x-Fold 1.1, SOC IC50 0.2 μM

Example 9 4,4′(phenyl-2-aminoethylthioboryl)diphenyl (6077)

TG 12, x-Fold 0.87, SOC IC50 0.5 μM

Example 10 4,4′(phenyl-2-aminoethoxyboryl)diphenyl (6076)

TG 7, x-Fold 0.92, SOC IC50 0.5 μM

Example 11 poly(2,5-dimethoxy-4-phenylborinic acid) (6047)

TG 36, x-Fold 0.99

Example 12 poly(aminoethyl-2,5-dimethoxy-4-phenylborinate) (6050)

TG 91, x-Fold 1.04

Example 13 poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylenehydroxyborane) (1122)

TG 100, x-Fold 1.11

4,4′-p-brombenzyl ether (90 mg) was dissolved in ether (4 ml), and themixture was cooled to −78° C. 1N sec-Butyllithium (0.75 mL) was addedand the mixture was stirred for 60 min (SOLUTION A).4,4′-parabromophenyl ether (90 mg) was dissolved in ether (4 ml), andthe mixture was cooled to −78° C. Thereto was added 1N sec-butyllithium(0.7 mL) and the mixture was stirred for 30 min. Triisopropoxyborane(188 mg) was added and the mixture was stirred to −65° C. (SOLUTION B).SOLUTION A and SOLUTION B were mixed, and the mixture was graduallywarmed and stirred at room temperature for 15 hr. The mixture wasacidified with 1N hydrochloric acid, and the organic layer was washedwith water, dried, and concentrated to give the title compound (154 mg).

Example 14 poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylene2-aminoethoxyborane) (1132)

TG 85, x-Fold 1.03

Poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylene hydroxyborane) (34mg) obtained in Example 13 was dissolved in a mixture of ethanol (0.5mL) and ether (0.5 mL) and the mixture was stirred at 50° C. for 1 hr.After concentration, ether (1 mL) was added to produce the titlecompound (15 mg) as a white precipitate.

Example 15 poly(4,4′-phenylenemethyleneoxymethylene4,4′-phenylene-dimethylaminoethoxyborinic acid) (1133)

TG 91, x-Fold 0.90

Example 16 poly(1,4-phenylenemethyleneoxymethylene 1,4-phenylene2-piperidinomethoxyborane) (1134)

TG 86, x-Fold 0.95

Example 17 poly(1,4-phenyleneoxy-1,4-phenylenehydroxyborane) (503)

TG 111, x-Fold 0.65

4,4′-Dibromodiphenylether (328 mg) was dissolved in ether (10 ml),sec-butyllithium (2 ml) was added at −95° C. and the mixture was warmedto −78° C. 30 min later. Thereto was added triisopropoxyborane (188 mg)and the mixture was stirred for 1 hr. The mixture was gradually warmedand stirred at room temperature for 15 hr. The mixture was acidifiedwith 1N hydrochloric acid, and the organic layer was washed with water,dried, concentrated, and subjected to silica gel column chromatographyto give the title compound (112 mg).

Example 18 poly(aminoethoxyboryldiphenylether) (1042D)

TG −17, x-Fold 0.84, SOC IC50 1.5 μM

Example 19 poly(isopropoxyboryldiphenylether) (1042E)

TG 47, x-Fold 0.86

Example 20 poly(4,4′-diphenylether dimethylaminoethoxyborane) (1056)

TG 54, x-Fold 0.63, SOC IC50 4 μM

Example 21poly(4,4′-diphenylether-2-pyridyl-4-trifluoromethylphenylmethoxyborane)(1120)

TG 111, x-Fold 0.72

Example 22 poly(4,4′-diphenylether-2-aminoethylthioborane) (1121)

TG 30, x-Fold 0.62

Example 23 poly(phenylenemethyleneoxyphenylenehydroxyborane) (1107)

TG 114, x-Fold 0.62

4,4′-p-bromophenyl p-brombenzyl ether (171 mg) was dissolved in ether (8ml), and the mixture was cooled to −100° C. Thereto was added 1Nsec-butyllithium (1 mL) and the mixture was stirred for 30 min to −78°C. (SOLUTION A). p-bromophenyl p-brombenzyl ether (171 mg) was dissolvedin ether (10 ml), and the mixture was cooled to −78° C. Thereto wasadded 1N sec-butyllithium (1 ml) and the mixture was stirred for 30 min.Triisopropoxyborane (188 mg) was added and the mixture was stirred to−65° C. (SOLUTION B). SOLUTION A and SOLUTION B were mixed, and themixture was gradually warmed and stirred at room temperature for 15 hr.The mixture was acidified with 1N hydrochloric acid, and the organiclayer was washed with water, dried, and concentrated to give the titlecompound (161 mg).

Example 24 poly(phenylenemethyleneoxyphenyleneaminoethoxyborane) (1116)

TG 96, x-Fold 0.78

Example 25 poly(phenylenemethyleneoxyphenyleneaminoethylthioborane)(1117)

TG 12, x-Fold 0.69

Example 26 poly(phenylenemethyleneoxyphenylenedimethylaminoethoxyborane)(1109)

TG 116, x-Fold 0.78

Example 27poly(4′-phenylhydroxyboranephenylenemethyleneoxyphenylenehydroxyboranephenylenemethyleneoxymethylene) (1108-3)

TG 45, x-Fold 0.86, SOC IC50 5 μM

The title compound (189 mg) was obtained from bis(4-bromobenzyl)ether(178 mg) and parabromophenyl parabrombenzyl ether (171 mg).

Example 28poly(phenylenemethyleneoxyphenyleneaminoethoxyboranephenylene-methyleneoxymethylenephenyleneaminoethoxyborane) (1114)

TG 94, x-Fold 0.72

Example 29poly(phenylenemethyleneoxyphenylenemethylaminoethoxyborane-phenylenemethyleneoxymethylenephenylenemethylaminoethoxyborane)(1115)

TG 52, x-Fold 0.83

Example 30 poly(4,4′-biphenylene-hydroxyborane1,4-phenylenemethyleneoxymethylenephenylenehydroxyborane) (1141c)

TG 107, x-Fold 1.02

Example 31 poly(4,4′-biphenylene 2-aminoethoxyborane1,4-phenylene-methyleneoxymethylene 1,4-phenylene 2-aminoethoxyborane)(1146)

TG 127, x-Fold 0.95

Example 32 di(3-chloro-4-methylphenyl)-2-aminoethylthioborane (3115)

TG 12, x-Fold 1.02, SOC IC50 1 μM

di(3-Chloro-4-methylphenyl)borinic acid (44 mg) and 2-aminoethanethiol(35 mg) were reacted in ethanol (1 mL) to give the title compound (52mg).

Example 33poly(2,5-dimethoxy-4-phenylene-hydroxyborane-1,4-phenylenehydroxyborane)(6048)

TG 51, x-Fold 0.92

Paradibromobenzene (353.85 mg) was dissolved in ether (10 mL), andsec-butyllithium (3 mL) was added at −95° C. 30 min later,triisoproxyborane (552 μL) was added at −78° C. and the mixture wasstirred for 1 hr (SOLUTION A). 2,5-Dimethoxy-1,4-dibromobenzene (443.35mg) was dissolved in ether (10 μL), sec-butyllithium (3 ml) was added at−95° C. and the mixture was stirred for 30 min (SOLUTION B). SOLUTION Aand SOLUTION B were mixed at −78° C., and the mixture was graduallywarmed to room temperature and stirred overnight. Thereto was addedhydrochloric acid solution to give the title compound (4.9 mg).

Example 34poly(aminoethyl(2,5-dimethoxy-4-phenylene)aminoethoxyboryl(1,4-phenylene)borinate)(6051)

TG 39, x-Fold 1.01

Example 35 poly(2-pyridylmethyl(2,5-dimethoxy-4-phenylene)2-pyridylmethoxyborane-(1,4-phenylene)borinate) (6053)

TG 14, x-Fold 0.98

Example 36 poly(4,4′-biphenylene-hydroxyborane 4,4′-diphenyletherhydroxyborane) (1068)

TG 6, x-Fold 0.65, SOC IC50 3 4M

4,4′-Dibromobiphenyl (312 mg) was dissolved in ether (10 mL), and themixture was cooled to −100° C. Thereto was added 1N sec-butyllithium(2.1 mL) and the mixture was stirred for 30 min to −78° C. (SOLUTION A).4,4′-Dibromodiphenylether (328 mg) was dissolved in ether (10 ml), andthe mixture was cooled to −78° C. Thereto was added 1N sec-butyllithium(2.1 ml) and the mixture was stirred for 30 min. Triisopropoxyborane(376 mg) was added and the mixture was stirred to −65° C. (SOLUTION B).SOLUTION A and SOLUTION B were mixed, and the mixture was graduallywarmed and stirred at room temperature for 15 hr. The mixture wasacidified with 1N hydrochloric acid, and the organic layer was washedwith water, dried, and concentrated to give the title compound (114 mg).

Example 37 poly(4,4′-biphenylene-dimethylaminoethoxyborane4,4′-diphenyletherdimethylaminoethoxyborane) (1074)

TG −22, x-Fold 0.73

Example 38 poly(4,4′-biphenylene-aminoethoxyborane-4,4′-diphenyletheraminoethoxyborane) (1077)

TG 79, x-Fold 0.71

Example 39 poly(phenyleneaminoethoxyboranediphenylether-aminoethoxyborane) (1060)

TG 99, x-Fold 1.04

Example 40 poly(phenyleneaminoethylthioboranediphenylether-aminoethylthioborane) (1062)

TG 26, x-Fold 0.52

Example 41 poly(phenylene 2-piperazinomethoxyborane diphenylether2-piperidinomethoxyborane) (1063)

TG 54, x-Fold 0.63, SOC IC50 2 μM

Example 42 poly(methylaminoethoxyborylphenylenemethylaminoethoxyboryldiphenylether) (1064)

TG 8, x-Fold 0.53, SOC IC50 2 μM

Example 43 poly(pyrrolidinomethoxyborylphenylenepyrrolidinomethoxyboryldiphenylether) (1065)

TG 13, x-Fold 0.73, SOC IC50 3 μM

Example 44 poly(aminoethylaminoethoxyborylphenyleneaminoethylaminoethoxyboryldiphenylether) (1066)

TG 12, x-Fold 0.54, SOC IC50 4 μM

Example 45poly(metaphenylene-hydroxyborane-4,4′-diphenyletherhydroxyborane) (1097)

TG 99, x-Fold 0.52

Example 46poly(metaphenylene-2-piperidinemethoxyborane-4,4′-diphenylether-2-piperidinemethoxyborane)(1102)

TG 93, x-Fold 0.50

Example 47 poly(metaphenylene-aminoethoxyborane-4,4′-diphenyletheraminoethoxyborane) (1103)

TG 106, x-Fold 0.58

Example 48poly(metaphenylene-methylaminoethoxyborane-4,4′-diphenylethermethylaminoethoxyborane)(1104)

TG 102, x-Fold 0.59

Example 49poly(metaphenylene-2-dimethylaminoethoxyborane-4,4′-diphenylether-2-dimethylaminoethoxyborane)(2102)

TG 89, x-Fold 0.96

Example 50poly(metaphenylene-2-pyridyl-trifluoromethylphenylmethoxyborane-4,4′-diphenylether-2-pyridyl-trifluoromethylphenylmethoxyborane)(1105)

TG 112, x-Fold 0.59

Example 51poly(metaphenylene-aminoethylthioborane-4,4′-diphenylether-aminoethylthioborane)(1106)

TG 13, x-Fold 0.43

Example 52 poly(4,4′-diphenyletherhydroxyboranephenylenemethyleneoxyphenylenehydroxyborane) (1069)

TG 73, x-Fold 0.69

Example 53poly(phenylenemethyleneoxyphenylene-aminoethoxyborane-4,4′-diphenyletheraminoethoxyborane)(1075)

TG 113, x-Fold 0.74

Example 54poly(phenyleneoxyphenylene-2-pyrrolidinemethoxyboryl-phenylenemethyleneoxyphenylene-2-pyrrolidinemethoxyborane)(1080)

TG 112, x-Fold 0.67

Example 55poly(phenylenemethyleneoxyphenylene-dimethylaminoethoxyborane-4,4′-diphenyletherdimethylaminoethoxyborane) (1081)

TG 151, x-Fold 0.71

Example 56poly(phenylenemethyleneoxyphenylene-2-pyridylmethoxyborane-4,4′-diphenylether-2-pyridylmethoxyborane)(1082)

TG 74, x-Fold 0.71

Example 57poly(4,4′-biphenylene-aminoethoxyborane-1,4-phenylene-methyleneoxy-1,4-phenylene-aminoethoxyborane)(1125)

TG 5.98, x-Fold 0.67, SOC IC50 μM

Example 58poly(4,4′-biphenylene-dimethylaminoethoxyborane-1,4-phenylene-methyleneoxyphenylenedimethylaminoethoxyborane)(1124)

TG 45, x-Fold 0.62

Example 59poly(4,4′-biphenylene-2-pyridylmethoxyborane-1,4-phenylene-methyleneoxy-1,4-phenylene-2-pyridylmethoxyborane)(1126)

TG 107, x-Fold 0.72

Example 60poly(4,4′-biphenylene-2-hydroxyethylaminoethoxyborane-1,4-phenylene-methyleneoxy-1,4-phenylene-2-hydroxyethylaminoethoxyborane)(1127)

TG 24, x-Fold 0.73

Example 61poly(4,4′-phenylene-methyleneoxymethylene-phenylene-hydroxyborane-4,4′-phenyleneoxyphenyleneborinicacid) (1123)

TG 100, x-Fold 0.99

4,4′-Dibromodibenzyl ether (96 mg) was dissolved in ether (6 ml), 1Msec-butyllithium (0.7 mL) was added and the mixture was stirred for 30min. Triisoproxyborane (240 μL) was added at −78° C. and the mixture wasstirred for 1 hr (SOLUTION A). 4,4′-Dibromodiphenyl ether (82.7 mg) wasdissolved in ether (5 ml), 1N sec-butyllithium (0.7 mL) was added at−78° C. and the mixture was stirred (SOLUTION B). SOLUTION A andSOLUTION B were mixed at −78° C., and the mixture was gradually warmedto room temperature and stirred overnight. 1N Hydrochloric acid wasadded, and the ether layer was washed with saturated brine, dried, andconcentrated to give the title compound (150 mg).

Example 62poly(phenylene-methyleneoxymethylene-phenylene-aminoethoxyborane-phenyleneoxyphenyleneaminoethoxyborane)(1135)

TG 94, x-Fold 0.95

Example 63 poly(phenylene methyleneoxymethylene phenylenedimethylaminoethoxyborane phenylene oxy phenylenedimethylaminoethoxyborane) (1136)

TG 63, x-Fold 1.04

Example 64 poly(phenylene methyleneoxymethylene phenyleneaminoethylthioborane phenylene oxy phenylene aminoethylthioborane)(1137)

TG 11, x-Fold 0.95

Example 65poly(diphenylene-methylaminoethoxyboryl-1,4-phenylene-methyleneoxymethylenephenylene-methylaminoethoxyborane)(1142)

TG 115, x-Fold 1.02, SOC IC50 7 μM

Example 66poly(1,4-phenylene-methyleneoxymethylenephenylenemethylaminoethoxyborane-1,4-phenylene-methylaminoethoxyborane)(1144)

TG 120, x-Fold 1.18, SOC IC50 >20 μM

Example 67poly(1,4-phenylene-methyleneoxymethylenephenylene-aminoethylaminoethoxyborane-1,4-phenylene-aminoethylaminoethoxyborane)(1145)

TG 122, x-Fold 0.87

4,4′-Parabrombenzyl ether (180 mg) was dissolved in ether (10 mL), andthe mixture was cooled to −78° C. 1.57N tert-Butyllithium (0.7 mL) wasadded and the mixture was stirred for 60 min (SOLUTION A).1,4-Dibromobenzene (118 mg) was dissolved in ether (10 mL), and themixture was cooled to −78° C. 1.57N tert-Butyllithium (0.7 mL) was addedand the mixture was stirred for 30 min. Triisopropoxyborane (188 mg) wasadded and the mixture was stirred to −65° C. (SOLUTION B). SOLUTION Aand SOLUTION B were mixed, and the mixture was gradually warmed to roomtemperature and stirred for 15 hr. The mixture was acidified with 1Nhydrochloric acid, and the organic layer was washed with water, dried,and concentrated to give the title compound (184 mg).

Example 68 polytetramethyleneborinic acid (6060)

TG 119, x-Fold 1.04

1,4-Tetramethylenedibromide (262 mg) was dissolved in ether (10 ml), andreacted with magnesium (Mg) (58 mg). Trimethoxyboroxin (60 μL) was addedand the mixture was stirred overnight. Hydrochloric acid was added andthe ether layer was concentrated to give the title compound (43.8 mg).

Example 69 2-dimethylaminoethyl bis(4-trifluoromethylphenyl)borinate(5034)

TG 76, x-Fold 1.02

Example 70 1,3-dimethylaminopropyl bis(3-chloro-4-methylphenyl)borinate(5141)

TG 13, x-Fold 0.73, SOC IC50 0.3 μM

Example 71 di(3-chloro-4-methylphenyl)(2,3-diaminopropionate-O,N)borane(5142)

TG 51, x-Fold 0.97, SOC IC50 1 μM

Example 72 di(3-chloro-4-methylphenyl)piperazinoethoxyborane (5143)

TG 41, x-Fold 1.02, SOC IC50 0.5 μM

Example 73 di(3-chloro-4-methylphenyl)piperidinoethoxyborane (5144)

TG 35, x-Fold 0.85, SOC IC50 1.2 μM

Example 74 di(3-chloro-4-methylphenyl)-2-piperidinoethoxyborane (5145)

TG 41, x-Fold 0.95, SOC IC50 1 μM

Example 75 bis(4-trifluoromethylphenyl)borinic acid (6001)

TG 97, x-Fold 0.88

Example 76 bis(3-chloro-4-fluorophenyl)borinic acid (6004)

TG 117, x-Fold 0.78

Example 77 2-aminoethyl-bis(3-chloro-4-fluorophenyl)borinate (6006)

TG 98, x-Fold 0.91

Example 78 2-dimethylaminoethyl bis(3-chloro-4-fluorophenyl)borinate(6007)

TG 104, x-Fold 1.02

Example 79 bis(4-chloro-2-fluorophenyl)borinic acid (6008)

TG 97, x-Fold 0.88

Example 80 bis(3,4-difluorophenyl)borinic acid (6009)

TG 93, x-Fold 0.90

Example 81 bis(3,4,5-trifluorophenyl)borinic acid (6010)

TG 97, x-Fold 0.92

Example 82 bis(2,4-difluorophenyl)borinic acid (6011)

TG 103, x-Fold 0.95

Example 83 bis(3-fluoro-4-chlorophenyl)borinic acid (6012)

TG 101, x-Fold 0.92

Example 84 2-aminoethyl bis(4-chloro-2-fluorophenyl)borinate (6013)

TG 91, x-Fold 0.92

Example 85 poly(4,4′-biphenylhydroxyborane) (504)

TG 128, x-Fold 0.79

4,4′-Dibromodiphenyl (234 mg) was dissolved in ether (10 ml), and 1.5Ntert-butyllithium (1.3 mL) was added at −95° C. 30 min later,triisoproxyborane (345 μL) was added at −78° C. and the mixture wasstirred for 1 hr (SOLUTION A). 4,4′-Dibromodiphenyl (234 mg) wasdissolved in ether (10 mL), 1.5N tert-butyllithium (1.3 mL) was added at−95° C. and the mixture was stirred (SOLUTION B). SOLUTION A andSOLUTION B were mixed at −78° C., and the mixture was gradually warmedto room temperature and stirred overnight. 1N Hydrochloric acid solutionwas added and the ether layer was with washed with saturated brine anddried and concentrated to give the title compound (155 mg).

Example 86 2-aminoethyl bis(3-chloro-4-fluorophenyl)borinate (6015)

TG 103, x-Fold 0.99

Example 87 2-aminoethyl bis(3,4-difluorophenyl)borinate (6016)

TG 91, x-Fold 1.02

Example 88 2-amino-1-phenylethyl bis(3,4-difluorophenyl)borinate (6017)

TG 82, x-Fold 0.83

Example 89 aminoethyl bis(3,4,5-trifluorophenyl)borinate (6018)

TG 80, x-Fold 0.94

Example 90 2-pyridylmethyl bis(3,4,5-trifluorophenyl)borinate (6019)

TG 93, x-Fold 0.81

Example 91 aminoethyl bis(3,5-difluorophenyl)borinate (6020)

TG 107, x-Fold 0.99

Example 92 dimethylaminoethyl bis(3,5-difluorophenyl)borinate (6021)

TG 106, x-Fold 1.00

Example 93 aminoethyl bis(4-chloro-3-fluorophenyl)borinate (6023)

TG 117, x-Fold 0.93

Example 94 dimethylaminoethyl bis(4-chloro-3-fluorophenyl)borinate(6024)

TG 114, x-Fold 0.95

Example 95 di(3-fluoro-4-chlorophenyl)(2,4-diaminolactonate-O,N)borane(6025)

TG 114, x-Fold 0.88

Example 96 di(3-fluoro-4-chlorophenyl)(glutaminate-O,N)borane (6026)

TG 124, x-Fold 0.86

Example 97 bis(3-chloro-5-fluorophenyl)borinic acid (6027)

TG 122, x-Fold 0.72

Example 98 bis(3-chloro-6-fluorophenyl)borinic acid (6029)

TG 111, x-Fold 0.95

Example 99 aminoethyl bis(3-chloro-5-fluorophenyl)borinate (6030)

TG 109, x-Fold 0.73

Example 100 aminoethyl bis(3-chloro-6-fluorophenyl)borinate (6032)

TG 119, x-Fold 0.97

Example 101 methylaminoethyl bis(3-chloro-6-fluorophenyl)borinate (6033)

TG 122, x-Fold 1.02

Example 102 bis(4-cyanophenyl)borinic acid (5009)

TG 72, x-Fold 1.10

Example 103 aminoethyl bis(4-cyanophenyl)borinate (6034)

TG 114, x-Fold 0.89

Example 104 2-pyridylmethyl bis(4-cyanophenyl)borinate (6037)

TG 94, x-Fold 1.16

Example 105 benzylaminoethyl bis(4-cyanophenyl)borinate (6038)

TG 92, x-Fold 1.05

Example 106 2-aminoethylthio bis(4-cyanophenyl)borane (6039)

TG 23, x-Fold 0.92

Example 107 secondary-butyl phenyl borinic acid (6040)

TG 111, x-Fold 0.98

Example 108 normal-butyl phenyl borinic acid (6041)

TG 111, x-Fold 1.00

Example 109 tertiary-butyl phenyl borinic acid (6042)

TG 108, x-Fold 1.02, SOC IC50 >10 μM

Example 110 aminoethyl secondary-butyl phenylborinate (6043)

TG 115, x-Fold 1.02, SOC IC50 >10 μM

Example 111 aminoethyl tertiary-butyl phenylborinate (6044)

TG 121, x-Fold 1.02

Example 112 aminoethyl normal-butyl phenylborinate (6046)

TG 123, x-Fold 0.99

Example 113 1,4-bis(hydroxyphenylboryl)butane (6059)

TG 112, x-Fold 0.99

Example 114 4-hydroxybutylphenylborinic acid (6059-9)

TG 120, x-Fold 0.99, SOC IC50 2 μM

Example 115 bis(4-chlorophenyl)borinic acid (385)

TG 101, x-Fold 1.07

Example 116 bis(di(3-chloro-4-methylphenyl)boryloxyethyl)piperazine(419)

TG 108, x-Fold 1.02

Example 117 bis(3-chloro-4-methylphenyl2-pyridylmethoxyborylphenyl)ether (434)

TG 108, x-Fold 0.06, SOC IC50 1.5 μM

Example 118 1,4-bis(phenyl-2-aminoethoxyboryl)benzene (544)

TG 93, x-Fold 0.97, SOC IC50 2 μM

Example 119 1,3-bis(phenylhydroxyboryl)benzene (554)

TG 101, x-Fold 0.84, SOC IC50 >20 μM

Example 120 1,3-bis(phenyl-2-aminoethoxyboryl)benzene (805)

TG 88, x-Fold 1.08

Example 121 1,2-bis(phenylhydroxyboryl)benzene (583)

TG 121, x-Fold 0.94

Example 122 diphenyl(argininate-O,N)borane (880)

TG 93, x-Fold 0.98, SOC IC50 7 μM

Arginine (82 mg) and 2-aminoethyldiphenylborinate (112 mg) were stirredin ethanol (0.4 ml), water (1.5 ml) and acetic acid (0.9 ml) at 110° C.for 3 hr to give the title compound (17 mg).

The present compound were also obtained by heating argininehydrochloride (211 mg) and sodium tetraphenylborate (342 mg) in water (5mL) at 100° C. for 3 hr.

Example 123 diphenyl(glutaminate-O,N)borane (870)

TG 98, x-Fold 0.84, SOC IC50 1 μM

Example 124(2-phenylhydroxyborylbenzyl)(3-(phenylhydroxyboryl)benzyl)ether (656)

TG 90, x-Fold 0.96

Example 125 bis(3-chloro-4-methylphenyl hydroxyborylbenzyl)ether (595)

TG 113, SOC IC50 10 μM

Example 126 bis(phenyl 2-pyridyl-4-methoxyphenylmethoxyborylbenzyl)ether(601)

TG 81, x-Fold 1.04

Example 127 bis(3-chloro-4-methylphenyl)2-pyridyl-4-methoxyphenylmethoxyborane (592)

TG 109, x-Fold 0.70

Example 128 1,4-bis(3-chloro-4-methylphenyl-2-aminoethoxyboryl)benzene(573)

TG 143, x-Fold 0.93

Example 129 di((phenylglycine-O,N boryl)phenyl)ether (1016)

TG 101, x-Fold 0.78

Example 130 1,3,5-tri(phenylhydroxyboryl)benzene (563)

TG 116, x-Fold 0.85

Example 131 bis((4,4′-phenylaminoethoxyboryl)benzyl)ether (163AE)

TG 16, x-Fold 1.1, SOC IC50 0.3 μM

Example 1321,3,5-tri(2-pyridyl-4-trifluoromethylphenylmethoxyphenylboryl)benzene(567)

TG 88, x-Fold 0.95

Example 133 (2-pyridyl-phenylmethoxyphenylboryl 2-benzyl)ether (566)

TG 106, x-Fold 1.00

Example 134 (2-pyridyl-4-trifluoromethylphenylmethoxyphenylboryl2-benzyl)ether (558)

TG 94, x-Fold 0.92

Example 135 1,4-bis(phenylhydroxyboryl)naphthalene (602)

TG 99, x-Fold 1.03

Example 136 diphenyl(asparaginate-O,N)borane (871)

TG 96, x-Fold 0.98

Example 137 bis((4,4′-phenylhydroxyboryl)benzyl)ether (163OH)

TG 14, x-Fold 0.99, SOC IC50 0.3 μM

Example 138 bis(2-pyridyl-4-trifluoromethylphenylmethoxyphenylboryl4-benzyl)ether (607)

TG 96, x-Fold 0.99

Example 139 bis(4-chloro-3-methylphenylhydroxyboryl 4-benzyl)ether (611)

TG 122, x-Fold 0.88

Example 140 4,4′-phenylhydroxyboryl 4-biphenyl (548)

TG −72, x-Fold 0.85

Example 141 bis(4,4′-(1-naphthylhydroxyboryl)benzyl)ether (620)

TG 97, x-Fold 0.92

Example 142 bis(4-fluorophenylhydroxyboryl 4-benzyl)ether (621)

TG 88, x-Fold 0.24

Example 143 bis(4-trifluoromethylphenylhydroxyboryl 4-benzyl)ether (618)

TG 118, x-Fold 0.90

Example 144 bis(3-chloro-4-methylphenylhydroxyboryl 4-benzyl)ether (612)

TG 99, x-Fold 0.87

Example 145 (3-chloro-4-fluorophenyl)boronic acid (6005)

TG 97, x-Fold 0.91

Example 146 1,4-bis(phenyl-2-aminoethoxyboryl) 2-methylbenzene (803)

TG 91, x-Fold 1.02

Example 147 1,3-bis(phenylhydroxyboryl)benzene (554)

TG 101, x-Fold 0.87, SOC IC50 20 μM

Example 148 bis(2,2′-(phenyl-2-aminoethoxyboryl)benzyl)ether (557)

TG 68, x-Fold 1.00

Example 149 4,4′-di((phenyl1-(pyridin-2-yl)-1-trifluoromethylphenylmethoxyboryl)benzyl)ether (607)

TG 96, x-Fold 0.99

Example 150 diphenyl-2-aminophenylthioborane (4122)

TG 2, x-Fold 0

Example 151 2-aminoethylthiodiphenylborane (1031)

TG 33, x-Fold 0.87

Example 152 di(4,4′-phenyldimethylaminoethoxyboryl)benzylether (1073)

TG54, x-Fold 1.07

Example 153poly(4,4′-biphenylene-2-pyridyl-4-trifluoromethylphenylmethoxyborane4,4′-diphenylether 2-pyridyl-4-trifluoromethoxyborane) (1079)

TG 65, x-Fold 0.79

Example 154 diphenyl 2-aminoethylaminoethyl borinate (1089)

TG 105, x-Fold 0.96-

Example 155 di(trifluoromethylphenyl) 2-pyridinomethylborinate (427)

TG 100, x-Fold 1.02

Example 156 di(3-chloro-6-methyl-phenyl)(argininate-O,N)borane (7138)

TG 91, x-Fold 1.08

Example 157 poly(phenylenemethyleneoxyphenyleneaminoethoxyborane) (1116)

TG 96, x-Fold 0.73

Example 158 poly(phenylenemethyleneoxyphenyleneaminoethylthioborane)(1117)

TG 12, x-Fold 0.69

Example 159 dibutyl(alanine-O,N)borane (926)

TG 102, x-Fold 0.96

Example 160 di(3-chloro-6-methyl-phenyl)(citrullinate-O,N)borane (7139)

TG 88, x-Fold 1.02

Example 161 FITC aminoethylaminoethyl diphenylborinate (1098)

TG 6, x-Fold 0.99

NHS-Florescein (Pierce: catalog No. 46100) (4.73 mg) was dissolved inDMF (100 μL), TEAB (pH 7.5) (100 μL) and diphenyl2-aminoethylaminoethoxyborane (2.68 mg) were added, and the mixture wasstirred at room temperature for 3 hr and applied to DEAE cellulosecolumn for purification, whereby the title compound (8.1 mg) wasobtained.

Example 162 tetramethylrhodamine aminoethylaminoethyl diphenylborinate(1099)

TG −2, x-Fold 0.85

Example 163 di(3-chloro-4-methylphenyl)N-methylpiperidinomethylborinate(347)

TG 109, x-Fold 1.00

Example 164 di(3-chloro-6-methylphenyl)benzylaminoethylborinate (376)

TG 94, x-Fold 0.67

Example 165 poly(4,4′-biphenylene-methylaminoethoxyborane 1,4-phenylenemethyleneoxymethylenephenylene-methylaminoethoxyborane) (1143)

TG 120, x-Fold 0.99

Example 166 di(3-chloro-6-methylphenyl)aminoethylborinate (372)

TG 74, x-Fold 0.70

Example 167(4-(phenyl-dimethylaminoethoxyboryl)phenyl)-(4′-(methoxymethoxymethylphenyl-dimethylaminoethoxyboryl)phenyl)ether(2006)

TG 21, x-Fold 0.71

Example 168(4-(phenyl-N-methylaminoethoxyboryl)phenyl)-(4′-(methoxymethoxymethylphenyl-N-methylaminoethoxyboryl)phenyl)ether(2007)

TG 35, x-Fold 0.72

Example 169 di((phenylglycine-O,N boryl)phenyl)ether (1016)

TG 101, x-Fold 0.78

Example 170 diphenyl(glycylglutamine-O,N)borane (907)

TG 96, x-Fold 0.96

Example 171 di(3-chloro-6-methylphenyl)borinic acid (370)

TG 98, x-Fold 0.71

Example 172 bis(3,3′(phenyldimethylaminoethoxyboryl)benzyl)ether (2024)

TG 69, x-Fold 1.22

Example 173 (3,3′-(phenylpiperazino-O,O-ethoxyboryl)benzyl)ether (2026)

TG 122, x-Fold 1.06

Example 174 diphenyl(2,3-diaminopropionate-O,N)borane (2031-4)

TG 103, x-Fold 0.99

Example 175 diphenyl(tetramethylrhodamine2,3-diaminopropionate-O,N)borane (2033)

TG 5, x-Fold 0.89

Example 176 diphenyl(tetramethylrhodamine2,6-diaminocapronate-O,N)borane (2035)

TG 47, x-Fold 1.06

Example 177 diphenyl(FITC-2,6-diaminocapronate-O,N)borane (2036)

TG 28, x-Fold 1.00

Example 178 diphenyl(2,3-diaminobutyrate-O,N)borane (2039)

TG 142, x-Fold 0.89

Example 179 diphenyl(2,5-diaminopentanate-O,N)borane (2044)

TG 127, x-Fold 0.99

Example 180 di(3-chloro-4-methylphenyl)(anthranate-O,N)borane (4124)

TG 35, x-Fold 0.98

Example 181 di(trifluoromethylphenyl) 2-aminoethylborinate (424)

TG 54, x-Fold 0.69

Example 182 di(3-chloro-4-methylphenyl)(glutaminate-O,N)borane (4105)

TG 137, x-Fold 1.01

Di(3-chloro-4-methylphenyl)borinic acid (32 mg) and glutamine (15 mg)were reacted in ethanol (0.6 mL) at 90° C. for 2 hr to give the titlecompound (34 mg).

Example 183 dibutyl(asparagine-O,N)borane (925)

TG 91, x-Fold 1.02

Example 184 di(4-(phenyl-2-pyridylmethoxyboryl)benzyl)ether (2049)

TG 94, x-Fold 0.95

Example 185bis(4,4′-(p-trifluoromethylphenyl-asparagineboryl)benzyl)ether (2064)

TG 130, x-Fold 0.94, SOC IC50 >20 μM

Aminoethyldiphenylborinate (112 mg) and piperazinecarboxylic acid (102mg) were reacted in ethanol (0.6 mL) and acetic acid (30 mL) at 80° C.for 5 hr to give the title compound (36 mg).

Example 186Di(1-(pyridin-2-yl)-1-(4-methoxyphenyl)methyl-phenyl-borylbenzyl)ether(601)

TG 81, x-Fold 0.98

Example 187 bis((4,4′-phenylhydroxyboryl)benzyloxybenzyl)hydroxyborane(2086)

TG 106, x-Fold 0.97

Example 188 di(trifluoromethylphenyl) 2-propylaminoethylborinate (428)

TG 91, x-Fold 0.98

Example 189bis((4,4′-phenylaminoethoxyboryl)benzyloxybenzyl)aminoethoxyborane(2088)

TG 119, x-Fold 0.94

Example 190 bis((4,4′-phenylmethylaminoethoxyboryl)benzyloxybenzyl)methylaminoethoxyborane (2089)

TG 99, x-Fold 1.05

Example 191bis((4,4′-phenyldimethylaminoethoxyboryl)benzyloxybenzyl)dimethylamino-ethoxyborane(2090)

TG 85, x-Fold 1.04

Example 192 bis((4,4′-phenyl2-pyridyl-4-trifluoromethylphenylmethoxyboryl)benzyloxybenzyl)2-pyridyl-4-trifluoromethyl phenylmethoxyborane (2091)

TG 102, x-Fold 0.95

Example 193 diphenyl(2-piperazine-3-carboxyamide-carboxy)borane (899)

TG 92, x-Fold 1.03

Aminoethyldiphenylborinate (112 mg) and pyrazine 2,3-dicarboxylic acidmonoamide (83 mg) were reacted in ethanol (0.5 mL) and acetic acid (30mg) to give the title compound (40 mg).

Example 194 diphenyl(methionate-O,N)borane (901)

TG 106, x-Fold 1.03

The title compound (35 mg) was obtained from diphenylborinic acid (61mg) and methionine (50 mg).

Example 195 di(phenyl 3-piperidinooxyboryl phenyl)ether (2108)

TG 115, x-Fold 0.77

Example 196 4,4′-(phenyl piperazino-O,O-ethoxyboryl)phenylether (2109)

TG 117, x-Fold 0.90

Example 197 4,4′-(phenyl piperazino-O,O-ethoxyboryl)benzylether (3001)

TG 99, x-Fold 1.02

Example 198 bis(4,4′-(phenyldimethylaminoethoxyboryl)benzyl)ether (3003)

TG 28, x-Fold 0.8

Example 199 bis(3,3′-(phenylbenzylaminoethoxyboryl)benzyl)ether (3017)

TG 3, x-Fold 0.90

Example 200 di(3-chloro-2-methylphenyl)borinic acid (442)

TG 100, x-Fold 0.92

Example 201 4,4′-di((3-chloro-4-methylphenyl 2-hydroxyboryl)phenyl)ether(431)

TG 99, x-Fold 0.57

Example 202 phenyl naphthyl 2-pyridylmethylborinate (3041)

TG 91, x-Fold 0.94

Example 203 phenyl naphthyl dimethylaminoethylborinate (3044)

TG 97, x-Fold 0.97

Example 204 phenyl naphthyl benzylaminoethylborinate (3045)

TG 61, x-Fold 0.79

Example 205 bis(4,4′-(phenyl 2-amino-2-benzylethoxyboryl)benzyl)ether(3087)

TG 47, x-Fold 0.80

Example 206 bis(3,3′-(phenyldimethylaminoethoxyboryl)benzyl)ether (3107)

TG 34, x-Fold 1.14

Example 207 di(3-chloro-4-methylphenyl)dimethylaminoethylborinate (3108)

TG 83, x-Fold 0.91

Example 208 di(3-chloro-4-methylphenyl)-2-benzyl-2-aminoethylborinate(3109)

TG −7, x-Fold 0.67

Example 209 di(3-chloro-4-methylphenyl)1-phenyl 2-aminoethylborinate(3111)

TG 1, x-Fold 0.98

Example 210 di(3-chloro-4-methylphenyl)butylaminoethyl borinate (3112)

TG 27, x-Fold 0.98, SOC IC50 2 μM

Example 211 di(3-chloro-4-methylphenyl)benzylaminoethyl borinate (3113)

TG 86, x-Fold 0.99, SOC IC50 1 μM

Example 212 diphenyl(R) 2-benzyl-2-aminoethyl borinate (3073)

TG 115, x-Fold 0.75

Example 213 diphenyl(S) 2-benzyl-2-aminoethyl borinate (3075)

TG 117, x-Fold 1.00

Example 214 di(3-chloro-4-methylphenyl) 1-phenylaminoethylborinate(3114)

TG −7, x-Fold 0.90, SOC IC50 2 μM

Example 215 di(3-chloro-4-methylphenyl)pyridylmethylborinate (3116)

TG 69, x-Fold 1.03, SOC IC50 2 μM

Example 216 di(3-chloro-4-methylphenyl)borinic acid anhydride (4139)

TG 17, x-Fold 1.03, SOC IC50 0.6 μM

Example 217 diphenylborinic acid anhydride (4111)

TG 118, x-Fold 0.94

Example 218 diphenyl(picolinate-O,N)borane (4118)

TG 90, x-Fold 0.97

Example 219 diphenyl(2-aminophenyl carboxylate-O,N)borane (4119)

TG 91, x-Fold 0.88

Example 220 di(3-chloro-4-methylphenyl) 2-aminophenylborinate (4121)

TG 26, x-Fold 0.50, SOC IC50 0.5 μM

Example 221 di(3-chloro-4-methylphenyl)(2-pyridinecarboxylate-O,N)borane (4123)

TG 73, x-Fold 0.94

Example 222 poly(4,4′-diphenylether glutamine-O,N borane) (8003)

TG 122, x-Fold 0.86

Compound 7142 (Example 478) (53.3 mg) and glutamine (44 mg) were reactedin ethanol (2 ml) at 80° C. for 24 hr to give the title compound (14mg).

NMR (DMSO) 1.95 (m, 2H), 2.0 (m, m, 2H), 2.23 (m, 2H), 3.35 (m, 4H),7.4-8.1 (m, 8H)

Example 223 poly(4,4′-diphenyl glutamine-O,N borane) (8006)

TG 116, x-Fold 1.02

Compound 4144 (Example 235) (41.3 mg) and glutamine (36 mg) were reactedin ethanol (2 ml) at 80° C. for 24 hr to give the title compound (75mg).

NMR (DMSO) 1.95 (m, 2H), 2.05 (m, 2H), 2.25 (m, 2H), 3.40 (m, 4H),6.8-7.7 (m, 8H)

Example 224 diphenyl 1-(2-aminobenzyl) 1-phenylmethylborinate (4127)

TG 112, x-Fold 0.89

Example 225 di(3-chloro-4-methylphenyl) 1-(2-aminobenzyl)1-phenylmethylborinate (4128)

TG 109, x-Fold 1.03, SOC IC50 0.5 μM

Example 226 diphenyl(2-aminohexanecarboxylate-O,N)borane (4129)

TG 97, x-Fold 0.94

Example 227 di(3-chloro-4-methylphenyl)(norloysinate-O,N)borane (4130)

TG 110, x-Fold 0.99

Example 228 diphenyl 2-aminobutylborinate (4131)

TG 99, x-Fold 0.98

Example 229 di(3-chloro-4-methylphenyl) 2-aminobutylborinate (4132)

TG 40, x-Fold 1.09, SOC IC50 0.5 μM

Example 230 di(trifluoromethylphenyl)borinic acid (4138)

TG 108, x-Fold 1.03

Example 231 di(3-fluoro-4-chlorophenyl)borinic acid (4140)

TG 94, x-Fold 1.01

Example 232 di(trifluoromethylphenyl) 2-aminoethylborinate (4141)

TG 108, x-Fold 1.10

Example 233 di(trifluoromethylphenyl) 2-dimethylaminoethylborinate(4142)

TG 112, x-Fold 1.12

Example 234 di(4-chloro-3-fluoro-phenyl) 2-aminoethylborinate (4143)

TG 98, x-Fold 1.07, SOC IC50 0.5 μM

Example 235 di(4-chloro-3-fluorophenyl) 2,3-diamino-2-propyl-borinate(4144)

TG 80, x-Fold 1.03

Example 236 di(4-chloro-3-fluorophenyl) 2-amino-2-methyl-propyl-borinate(4145)

TG 87, x-Fold 1.10

Example 237 di(4-chloro-3-fluorophenyl) 2-phenylaminoethyl borinate(4146)

TG 88, x-Fold 1.15

Example 238 di(4-chloro-3-fluorophenyl) 2-amino-3-hydroxybutyl borinate(4147)

TG 87, x-Fold 1.07

Example 239 bis(diphenyl piperazino-O,O-ethoxyborane) (356)

TG 126, x-Fold 0.94

Example 2404-((2-aminoethoxy)phenylboryl)benzyl-4′-((2-aminoethoxy)phenylboryl)phenethylether(7117)

TG 25, x-Fold 0.99, SOC IC50 0.08 μM

Example 241 di(3-chlorophenyl)borinic acid (244)

TG 67, x-Fold 1.10

Example 242 di(5-chloro-2-methylphenyl) 2-piperidinomethylborinate (371)

TG 98, x-Fold 1.17

Example 243 di((5-chloro-2-methylphenyl)hydroxyborylphenyl)ether (436)

TG 106, x-Fold 0.73

Example 244 di(5-chloro-2-methylphenyl) 2-aminoethylborinate (372)

TG 74, x-Fold 0.76, SOC IC50 1 μM

Example 245 diphenyl(ornithine-O,N)borane (921)

TG 94, x-Fold 0.91

Example 246 di(5-chloro-2-methylphenyl) 2-butylaminoethylborinate (376)

TG 94, x-Fold 0.67

Example 247 di(3-chloro-4-methylphenyl) 2-piperidinomethylborinate (422)

TG 99, x-Fold 0.91, SOC IC50 0.7 μM

Example 248 di(3-chloro-4-methylphenyl) 2-piperidinoethylborinate (421)

TG 103, x-Fold 0.87

Example 2494,4′-((2-aminoethoxy)(3-chloro-4-methylphenyl)boryl)diphenylether (7118)

TG 25, x-Fold 0.74, SOC IC50 0.3 μM

Example 250 bis(4,4′-(phenyldimethylaminoethoxyboryl)phenyl)ether (1007)

TG 125, x-Fold 0.86

Example 251 bis(3-chloro-4-methylphenyl hydroxyborylphenyl)ether (488)

TG 121, x-Fold 0.83

Example 252 1,4-bis(phenylhydroxyboryl)benzene (542)

TG 93, x-Fold 0.95, SOC IC50 0.5 μM

Example 253 di(2-thiophene)borinic acid (283)

TG 92, x-Fold 1.11

Example 254 diphenyl(glycinate-O,N)borane (827)

TG 101, x-Fold 0.95

Example 255 diphenyl(serinate-O,N)borane (828)

TG 113, x-Fold 0.94, SOC IC50 0.5 μM

Example 256 diphenyl(glutaminate-O,N)borane (829)

TG 112, x-Fold 0.67, SOC IC50 1.5 μM

Diphenylborinic acid (78 mg) and sodium glutamate (73 mg) were stirredwith heating in ethanol, water 1:1 mixture (1 mL) at 70° C. for 1 hr togive the title compound (120 mg).

Example 257 diphenyl(asparaginate-O,N)borane (830)

TG 103, x-Fold 0.98

Diphenylborinic acid (50 mg) and aspartic acid (25 mg) were stirred withheating in ethanol, water 1:1 mixture (1 mL) at 70° C. for 1 hr to givethe title compound (6 mg).

Example 258 diphenyl(alaninate-O,N)borane (833)

TG 110, SOC IC50 5 μM

Diphenylborinic acid (50 mg) and L-alanine (25 mg) were stirred withheating in ethanol, water 1:1 mixture (1 mL) at 70° C. for 1 hr to givethe title compound (6 mg).

Example 259 diphenyl(phenylalaninate-O,N)borane (841)

TG 67, x-Fold 0.97, SOC IC50 2.5 μM

Diphenylborinic acid (47 mg) and phenylalanine (43 mg) were stirred withheating in ethanol, water 1:1 mixture (1 mL) at 70° C. for 16 hr to givethe title compound (10 mg).

Example 260 diphenyl(tryptophanate-O,N)borane (836)

TG 106, x-Fold 0.89

Diphenylborinic acid (46 mg) and tryptophan (52 mg) were stirred withheating in ethanol, water 1:1 mixture (1 mL) at 70° C. for 1 hr to givethe title compound (15 mg).

Example 261 diphenyl(leucinate-O,N)borane (837)

TG 109, x-Fold 0.89

Diphenylborinic acid (46 mg) and leucine (33 mg) were stirred withheating in ethanol, water 1:1 mixture (1 ml) at 70° C. for 1 hr to givethe title compound (10 mg).

Example 262 diphenyl(isoleucinate-O,N)borane (838)

TG 115, x-Fold 0.97

Diphenylborinic acid (52 mg) and isoleucine (37 mg) were stirred withheating in ethanol, water 1:1 mixture (1 mL) at 70° C. for 1 hr to givethe title compound (10 mg).

Example 263 diphenyl(2,4-diaminolactonate-O,N)borane (2045)

TG 146, x-Fold 0.89, SOC IC50 3 μM

Sodium tetraphenylborate (342 mg) and 2,4-diaminobutyricacid-hydrochloride (191 mg) were stirred with heating in water (7 ml) at80° C. for 1 hr to give the title compound (160 mg).

Example 264 diphenyl(tyrosinate-O,N)borane (842)

TG 109, x-Fold 1.00, SOC IC50 5 μM

Diphenylborinic acid (57 mg) and tyrosine (57 mg) were stirred withheating in ethanol, water 1:1 mixture (1 ml) at 70° C. for 1 hr to givethe title compound (24 mg).

Example 265 diphenyl(threoninate-O,N)borane (851)

TG 112, x-Fold 0.94

Diphenylborinic acid (42 mg) and threonine (28 mg) were stirred withheating in ethanol, water 1:1 mixture (0.5 mL) at 70° C. for 1 hr togive the title compound (20 mg).

Example 266 diphenyl(cysteinate-O,N)borane (847)

TG 84, x-Fold 0.87, SOC IC50 3 μM

Diphenylborinic acid (31 mg) and cysteine (21 mg) were stirred withheating in ethanol, water 1:1 mixture (0.5 mL) at 70° C. for 1 hr togive the title compound (20 mg).

Example 267 diphenyl(histidinate-O,N)borane (848)

TG 82, x-Fold 0.60, SOC IC50 3 μM

Diphenylborinic acid (32 mg) and histidine hydrochloride (36 mg) werestirred with heating in ethanol, water 1:1 mixture (0.5 mL) at 70° C.for 1 hr to give the title compound (6 mg).

Example 268 diphenyl(hydroxyprolinate-O,N)borane (852)

TG 103, x-Fold 0.96, SOC IC50 5 μM

Diphenylborinic acid (41 mg) and hydroxyproline (30 mg) were stirredwith heating in ethanol, water 1:1 mixture (0.5 ml) at 70° C. for 1 hrto give the title compound (5 mg).

Example 269 diphenyl(glutaminate-O,N)borane (879)

TG 95, x-Fold 1.01, SOC IC50 3 μM

Diphenyl 2-aminoethylborinate (112 mg) and glutamine (74 mg) werestirred with heating in a mixture of ethanol (0.4 mL), water (1.5 ml)and acetic acid (0.03 ml) at 100° C. for 10 min to give the titlecompound (21 mg).

Example 270 diphenyl(asparaginate-O,N)borane (855)

TG 111, x-Fold 0.54, SOC IC50 0.7 μM

Diphenylborinic acid (182 mg) and asparagine (32 mg) were stirred withheating in ethanol, water 3:1 mixture (1 mL) at 70° C. for 1 hr to givethe title compound (14 mg).

Example 271 diphenyl(lysinate-O,N)borane (906)

TG 109, x-Fold 1.07, SOC IC50 0.5 μM

Diphenylborinic acid (49 mg) and lysine hydrochloride (49 mg) werestirred with heating in a mixture of ethanol (1.5 ml) and water (0.5 mL)at 80° C. for 1 hr to give the title compound (44 mg).

Example 272 diphenyl(2,3-diaminopropionate-O,N)borane (2043)

TG 83, x-Fold 0.09, SOC IC50 0.3 μM

Sodium tetraphenylborate (342 mg) and 2,4-diaminopropionicacid•hydrochloride (141 mg) were stirred with heating in water (5.5 ml)at 80° C. for 2 hr to give the title compound (203 mg).

Example 273 bis(4,4′-(phenyl-glutamineboryl)phenyl)ether (1024)

TG 83, x-Fold 0.56, SOC IC50 0.25 μM

Bis(4,4′-(phenylhydroxyboryl)phenyl)ether (22 mg) and glutamine (19 mg)were heated in ethanol (2 mL) at 60° C. for 1 hr to give the titlecompound (8 mg).

Example 274 bis(4,4′-(phenylasparagineboryl)phenyl)ether (1023)

TG 56, x-Fold 0.59, SOC IC50 0.3 μM

Bis(4,4′-(phenylhydroxyboryl)phenyl)ether (20 mg) and asparagine (14 mg)were stirred with heating in ethanol (3 mL) at 60° C. for 1 hr to givethe title compound (7 mg).

Example 275 (4-(phenyl-glutamic acidboryl)phenyl)-(4′-(hydroxymethylphenyl-glutamic acid boryl)phenyl)ether(1036)

TG 117, x-Fold 0.67, SOC IC50 0.3 μM

4-(Phenyl-hydroxyboryl)phenyl)-4′-(hydroxymethylphenyl-hydroxyboryl)phenyl)ether(27 mg) and sodium glutamate (22.3 mg) were reacted in ethanol (0.5 mL)to give the title compound (23 mg).

Example 276 diphenyl(glutaminate-O,N)borane (854)

TG 105, x-Fold 0.8

Diphenylborinic acid (39 mg) and glutamine (3.7 mg) were reacted inethanol (0.6 mL) at 60° C. for 1 hr to give the title compound (10 mg).

Example 277 diphenyl(prolinate-O,N)borane (843)

TG 105, x-Fold 0.98, SOC IC50 0.3 μM

Diphenylborinic acid (47 mg) and proline (2.7 mg) were reacted inethanol (0.6 ml) at 60° C. for 1 hr to give the title compound (10 mg).

Example 278(3-phenoxybenzyl)-(3′-(phenyl-2-aminoethoxyboryl)benzyl)ether (7119)

TG 2, x-Fold 1.08, SOC IC50 0.3 μM

Using 3-bromobenzyl-3′-phenoxybenzylether (1173 mg), bromobenzene (400mg) and triisopropoxyborane (560 mg) as main starting materials,hydroxybromo compound was synthesized, and reacted with ethanolamine atroom temperature to give the title compound (700 mg).

NMR (CDCl₃), 2.73 (m, 2H), 3.72 (t, 2H), 4.14 (m, 4H), 4.49 (s, 2H),6.8-7.3 (m, 18H)

Example 279 diphenyl(2-piperazinecarboxy)borane (894)

TG 103, x-Fold 0.98

Example 280 diphenyl(2,4-diaminolacetic acid)borane (897)

TG 98, x-Fold 0.88

Aminoethyldiphenylborinate (112 mg) and 2,4-diaminobutyricacid•hydrochloride (35 mg) were reacted in ethanol (0.5 ml) and aceticacid (30 mg) to give the title compound (139 mg).

Example 281 di(3-chloro-4-methylphenyl)-(picolinate-O,N)borane (4123)

TG 77, x-Fold 0.94

Example 282 di(3-chloro-4-methylphenyl)(asparaginate-O,N)borane (4103)

TG 112, x-Fold 0.95, SOC IC50 0.3 μM

Di(3-chloro-4-methyl)phenylborinic acid (82 mg) and asparagine (81 mg)were reacted in ethanol (0.6 mL) to give the title compound (37 mg).

Example 283 di(3-chloro-4-methylphenyl) 2-aminophenylthioborane (4125)

TG 12, x-Fold 0.83, SOC IC50 0.9 μM

Di(3-chloro-4-methyl)phenylborinic acid (47 mg) anddimethylaminoethanethiol (17 mg) were stirred in ether (1 ml) overnight,ether (2 ml) was added to give the title compound (17 mg) as a whiteprecipitate.

Example 284 di(4-trifluoromethylphenyl) (picolinate-O,N)borane (5003)

TG 89, x-Fold 1.03

Example 285 di(4-trifluoromethylphenyl) 2-aminoethylthioborane (5004)

TG 51, x-Fold 0.99, SOC IC50 2 μM

Example 286 di(3-chloro-4-methylphenyl)(2,6-diaminopimelinate-O,N)borane(5012)

TG 104, x-Fold 0.93

Example 287 di(3-chloro-4-methylphenyl)(citrullinate-O,N)borane (5013)

TG 146, x-Fold 1.00

Example 288 di(3-chloro-4-methylphenyl)(glycylglutaminate-O,N)borane(5014)

TG 106, x-Fold 1.02

Example 289di(4-trifluoromethylphenyl)(1,3-propylenediaminediacetate-O,N)borane(5015)

TG 94, x-Fold 1.08, SOC IC50 0.3 μM

Example 290 di(4-trifluoromethylphenyl)(glycylglycinate-O,N)borane(5018)

TG 113, x-Fold 1.05

Example 291 di(3-chloro-4-methylphenyl)(allothreoninate-O,N)borane(5019)

TG 50, x-Fold 1.02, SOC IC50 0.5 μM

Example 292 di(3-chloro-4-methylphenyl)(norloysinate-O,N)borane (5020)

TG 146, x-Fold 1.00, SOC IC50 1 μM

Example 293 di(3-chloro-4-methylphenyl)(2,4-diaminobutyrate-O,N)borane(5021)

TG 116, x-Fold 0.91

Example 294 diphenyl dimethylaminoethylthioborane (4106)

TG 114, x-Fold 0.96, SOC IC50 2 μM

1N Sodium hydroxide (0.28 mL) was added to dimethylaminoethylthiolhydrochloride (40 mg) and the mixture was extracted with ether.Diphenylborinic acid (44 mg) was added and the mixture was dried tosolidness, ethanol (1 mL) was added and the mixture was stirred for 15hr, dried to solidness and washed with ether to give the title compound(2 mg).

Example 295 di(3-chloro-4-methylphenyl)dimethylaminoethylthioborane(4107)

TG 107, x-Fold 0.92, SOC IC50 0.8 μM

Example 296(4-(2-thiophenehydroxyboryl)phenoxyethyl)(4′-(2-thiophenehydroxyboryl)benzyl)ether(795)

TG 97, x-Fold 0.74

Example 297 1,2-di(phenylhydroxyboryl)benzene (806)

TG 89, x-Fold 0.69

1,2-Dibromobenzene (236 mg) was reacted with 1N sec-BuLi (2.1 mL) at−98° C. (SOLUTION A). Bromobenzene was reacted with sec-BuLi andtriisopropoxyborane (460 μL) (SOLUTION B). SOLUTION A and SOLUTION Bwere reacted to give the title compound (95 mg) as a candy-likesubstance.

Example 298 1,2-di(phenylaminoethoxyboryl)benzene (810)

TG 101, x-Fold 1.01

Example 299 poly(2,5-dimethylphenyl asparagine-O,N borane) (8007)

TG 118, x-Fold 1.13

Poly(2,5-dimethylphenyl hydroxyborane) (34 mg) and glutamine (40 mg)were stirred in ethanol at 80° C. for 12 hr to give the title compound(7 mg).

NMR (DMSO) 1.95 (m, 2H), 2.0 (m, 2H), 2.1 (m, 6H), 3.2 (m, 4H), 7.2-8.0(m, 2H)

Example 300 poly(phenylene 2-aminoethylaminoethoxy borane) (1085)

TG 95, x-Fold 0.80, SOC IC50 5 μM

Example 301 poly(phenylene 2-pyridylmethoxy borane) (1083)

TG 108, x-Fold 0.84

Example 302 poly(1,4-phenylenehydroxyboryl-1,3-phenyleneborinic acid)(6062)

TG 103, x-Fold 0.94

Example 303 poly(1,4-phenyleneaminoethoxyboryl-1,3-phenyleneaminoethoxyborane) (6082)

TG 103, x-Fold 0.91

Example 304 2,8-di(3-thiophenylglutamine-O,N boryl)dibenzothiophene(8020)

TG 47, x-Fold 0.90

Compound 8013 (Example 406) (24 mg) and glutamine (19 mg) were stirredin ethanol at 80° C. for 12 hr to give the title compound (16 mg).

NMR (DMSO) 1.90 (m, 2H), 1.95 (m, 2H), 2.10 (m, 4H), 2.30 (m, 4H),7.0-8.0 (m, 12H)

Example 305 4,4′-di(cyano-phenyl)borinic acid (6095)

TG 94, x-Fold 0.98

Example 306 3,3′-di(cyano-phenyl)borinic acid (6096)

TG 90, x-Fold 0.98

Example 307 diphenyl(citrullinate-O,N)borane (7021)

TG 54, x-Fold 1.06, SOC IC50 0.5 μM

Example 308 diphenyl(ornithinate-O,N)borane (7020)

TG 27, x-Fold 1.05, SOC IC50 0.5 μM

Example 309 poly(1,2-phenylene-hydroxyborane) (7047)

TG 109, x-Fold 0.93

Example 310 poly(2,5-dimethyl-1,4-phenylene-hydroxyborane) (7051)

TG 114, x-Fold 1.02

2,5-Dimethyl-1,5-dibromobenzene (263 mg) was dissolved in ether (10 mL)at −78° C., sec-butyllithium (2 ml) was added and the mixture wasstirred for 1 hr. Triisopropoxyborane (220 μL) was added and the mixturewas gradually warmed to room temperature and treated with hydrochloricacid to give the title compound (74.5 mg).

NMR (CDCl₃) 2.38 (s, 6H), 7.4 (m, 2H)

Example 311 poly(2-methyl-1,3-phenylene-hydroxyborane) (7052)

TG 111, x-Fold 1.00

Example 312 poly(2,8-dibenzothiophenylene-hydroxyborane) (7053)

TG 98, x-Fold 1.00

Example 313 poly(2,2′-biphenylene-hydroxyborane) (7056)

TG 107, x-Fold 0.98

Example 314 poly(1,4-naphthalene-hydroxyborane) (7057)

TG 104, x-Fold 0.93

4,4′-parabrombenzylether (90 mg) was dissolved in ether (4 mL), and 1Nsec-butyllithium (0.75 mL) cooled to −78° C. was added and the mixturewas stirred for 60 min (SOLUTION A). 4,4′-parabromophenylether (90 mg)was dissolved in ether (4 mL) and the mixture was cooled to −78° C. 1Nsec-Butyllithium (0.7 mL) was added and the mixture was stirred for 30min. Triisopropoxyborane (188 mg) was added and the mixture was stirredto −65° C. (SOLUTION B). SOLUTION A and SOLUTION B were mixed and themixture was gradually warmed and stirred at room temperature for 15 hr.The mixture was acidified with 1N hydrochloric acid, and the organiclayer was washed with water, dried, and concentrated to give the titlecompound (154 mg).

Example 315 poly(9,10-anthracene-hydroxyborane) (7058)

TG 102, x-Fold 0.92

Example 316 poly(3,6-carbazole-hydroxyborane) (7059)

TG 72, x-Fold 1.11

Example 317 poly(5-methyl-1,3-phenylene-hydroxyborane) (7063)

TG 107, x-Fold 0.99

Example 318 poly(5,5′-bithiophene-hydroxyborane) (7064)

TG 81, x-Fold 1.02

Example 319 poly(2,2′-binaphthyl-hydroxyborane) (7065)

TG 108, x-Fold 1.04

Example 320 poly(4,4′-biphenylene aminoethoxyborane) (1128)

TG 100, x-Fold 0.78, SOC IC50 5 μM

Poly(4,4′-biphenylborinic acid) (38 mg) was dissolved in ether (0.5 mL),ethanolamine (13 mg) was added and the mixture was stirred for 10 hr.Ether (1 mL) was added to give the title compound (12 mg) as aprecipitate.

Example 321 poly(4,4′-biphenylene N-hydroxyethylaminoethoxyborane)(1129)

TG 116, x-Fold 0.78

Example 322 bis(4,4′-(3-chloro-4-methylphenylhydroxyboryl)benzyl)ether(612)

TG 98, x-Fold 0.32, SOC IC50 0.2 μM

Example 323 poly(4-phenylborinic acid) (502)

TG 111, x-Fold 0.82

Paradibromobenzene (148 mg) was dissolved in ether (10 ml),sec-butyllithium (1.5 mL) was added at −95° C. and the mixture wasstirred for 30 min. Triisoproxyborane (276 μL) was added at −78° C. andthe mixture was stirred for 1 hr (SOLUTION A). Paradibromobenzene (148mg) was dissolved in ether (10 mL), sec-butyllithium (1.5 ml) was addedat −95° C. and the mixture was stirred for 30 min (SOLUTION B). SOLUTIONA and SOLUTION B were mixed at −78° C., and the mixture was graduallywarmed to room temperature and stirred overnight. Hydrochloric acidsolution was added, and the mixture was applied to column chromatographyto give the title compound (110 mg).

Example 324 naphthaleneboronic acid (7126)

x-Fold 0.76

Example 325 bis(4-(4-trifluoromethylphenylhydroxyboryl)phenyl)ether(2054)

TG 92, x-Fold 0.99, SOC IC50 4 μM

Example 326 poly(2,5-dimethylphenyl aminopropoxyborane) (8009)

TG 103, x-Fold 1.09

Compound 7051 (Example 310) (34 mg) and ethanolamine (17 mg) werereacted at room temperature for 4 hr to give the title compound (8.7mg).

NMR (CDCl₃) 2.34 (s, 6H), 2.62 (m, 2H), 2.95 (m, 2H), 3.65 (m, 2H),7.2-7.8 (m, 2H)

Example 327 poly(2,5-dimethylphenyl aminopropylthioborane) (8010)

TG 14, x-Fold 1.07

Compound 7051 (Example 310) (32 mg) and aminoethanethiol (20 mg) werereacted at room temperature for 4 hr to give the title compound (28 mg).

NMR (CDCl₃) 1.8-2.0 (br, 2H), 2.31 (m, 6H), 2.76 (m, 2H), 3.01 (m, 2H)

Example 328 bis(3-(4-methoxyphenylhydroxyboryl)benzyl)ether (2072)

TG 100, x-Fold 1.04

Example 329(3-(phenylhydroxyboryl)benzyl)(4-(phenylhydroxyboryl)benzyl)ether (672)

TG 81, SOC IC50 0.2 μM

Example 330(2-(phenylhydroxyboryl)benzyl)(3-(phenylhydroxyboryl)benzyl)ether (655)

TG 89, x-Fold 0.90

Example 331(2-(phenylhydroxyboryl)benzyl)(4-(phenylhydroxyboryl)benzyl)ether (682)

TG 101, x-Fold 0.98, SOC IC50 1 μM

Example 332(3-(phenylaminoethoxyboryl)benzyl)(4-(phenylaminoethoxyboryl)benzyl)ether(674)

TG 21, x-Fold 0.98, SOC IC50 0.2 μM

Example 333 bis(3-(3-chloro-4-methylphenylhydroxyboryl)benzyl)ether(701)

TG 107, x-Fold 1.09

Example 334(2-(phenylaminoethoxyboryl)benzyl)(3-(phenylaminoethoxyboryl)benzyl)ether(687)

TG 21, x-Fold 1.02, SOC IC50 0.3 μM

Example 335 (2-(phenylaminoethoxyboryl)benzyl)(4-(phenylaminoethoxyboryl)benzyl)ether (686)

TG 91, x-Fold 1.02

Example 336 bis(3-(4-fluorophenylhydroxyboryl)benzyl)ether (688)

TG 101, x-Fold 1.02

Example 337 bis(3-(4-fluorophenylaminoethoxyboryl)benzyl)ether (689)

TG 102, x-Fold 0.98

Example 338 bis(4-(4-chloro-3-methyl-phenyl)hydroxyborylbenzyl)ether(693)

TG 110, x-Fold 0.83

Example 339 bis(4-(4-chloro-3-methyl-phenylaminoethoxyborylbenzyl)ether(696)

TG 115, x-Fold 0.91

Example 340 bis(3-(3′,4′-methylenedioxy-phenylhydroxyboryl)benzyl)ether(700)

TG 63, x-Fold 1.01

Example 341(3-(3-chloro-4-methylphenylhydroxyboryl)benzyl)(4-(3-chloro-4-methylphenylhydroxyboryl)benzyl)ether(701)

TG 107, x-Fold 1.04

Example 342(3-(3′,4′,5′-trifluorophenylhydroxyboryl)benzyl)(4-(3′,4′,5′-trifluorophenylhydroxyboryl)benzyl)ether(702)

TG 114, x-Fold 1.02

Example 343 bis(3-(4-methoxyphenylaminoethoxyboryl)benzyl)ether (704)

TG 55, x-Fold 1.02

Example 344(3-(4-chloro-3-methylphenylhydroxyboryl)benzyl)(2-(4-chloro-3-methylphenylhydroxyboryl)benzyl)ether(705)

TG 91, x-Fold 0.93

Example 345 bis(3-(4-cyanophenylhydroxyboryl)benzyl)ether (706)

TG 95, x-Fold 0.92

Example 346 bis(3-(2′-thiophenylhydroxyboryl)benzyl)ether (707)

TG 101, x-Fold 0.81

Example 347 bis(3-(1′-naphthylhydroxyboryl)benzyl)ether (708)

TG 104, x-Fold 0.90

Example 349 bis(4-(2-methoxy-5-fluorophenylhydroxyboryl)benzyl)ether(710)

TG 104, x-Fold 0.80

Example 350 bis(4-(2-methoxy-5-fluorophenylaminoethoxyboryl)benzyl)ether(717)

TG 105, x-Fold 0.92

Example 351(3-(4-chloro-3-methyl-phenylaminoethoxyboryl)benzyl)(2-(4-chloro-3-methyl-phenylaminoethoxyboryl)benzyl)ether(711)

TG 103, x-Fold 1.00

Example 352 bis(4-(3,4-difluorophenylhydroxyboryl)benzyl)ether (718)

TG 97, x-Fold 1.02

Example 353 bis(4-(3,4-difluorophenylaminoethoxyboryl)benzyl)ether (712)

TG 115, x-Fold 0.85

Example 354(3-(3′,4′,5′-trifluorophenylaminoethoxyboryl)benzyl)(4-(3′,4′,5′-trifluorophenylaminoethoxyboryl)benzyl)ether(719)

TG 113, x-Fold 1.09

Example 355 5,5′-(phenylhydroxyboryl)-2,2′-dithiophene (731)

TG 91, x-Fold 1.09

Example 356 5,5′-(phenylaminoethoxyboryl)-2,2′-dithiophene (735)

TG 51, x-Fold 1.06

Example 357 3,5-di(phenylaminoethoxyboryl)toluene (736)

TG 89, x-Fold 1.03

Example 358 2,5-di(phenylhydroxyboryl)toluene (739)

TG 112, x-Fold 0.91

Example 359 2,2′-di(phenylhydroxyboryl)-1,1′-binaphthyl (744)

TG 139, x-Fold 0.96

Example 360 2,2′-di(phenylaminoethoxyboryl)-1,1′-binaphthyl (745)

TG 88, x-Fold 1.05

Example 361 bis(4-(4-methylphenylhydroxyboryl)benzyl)ether (709)

TG 100, x-Fold 0.88, SOC IC50 >20 μM

Example 362 bis(4-(4-methylphenylaminoethoxyboryl)benzyl)ether (729)

TG 108, x-Fold 1.08

Example 363 4,4′-(4-methylphenylhydroxyboryl)diphenyl (752)

TG 97, x-Fold 0.92

Example 364 4,4′-(4-methylphenylaminoethoxyboryl)diphenyl (754)

TG 44, x-Fold 0.82

Example 365 4,4′-(4-methylphenylhydroxyboryl)diphenylether (753)

TG 118, x-Fold 0.91

Example 366 poly(2,5-dimethylphenyl 2-pyridylmethoxyborane) (8011)

TG 108, x-Fold 0.93

Compound 7051 (Example 310) (7.2 mg) and 2-pyridylmethanol (6 mg) werereacted in ethanol at room temperature for 4 hr to give the titlecompound (4 mg).

NMR (CDCl₃) 3.45 (m, 6H), 4.72 (m, 2H), 7.2-8.5 (m, 6H)

Example 367 4,4′-bis(3-chloro-4-methyl-phenylhydroxyboryl)diphenylether(513)

TG 113, x-Fold 0.73

Example 368(2-(phenylhydroxyboryl)phenethyl)((2-phenylhydroxyboryl)benzyl)ether(6055)

TG 52, x-Fold 1.03

Example 369(2-(phenylaminoethoxyboryl)phenethyl)((2-phenylaminoethoxyboryl)benzyl)ether(7133)

TG 105, x-Fold 1.10

Example 370(4-phenylhydroxyborylphenyl)(4′-phenylhydroxyborylbenzyl)ether (775)

TG 39, x-Fold 0.76, SOC IC50 2 μM

Example 371(4-phenylaminoethoxyborylphenyl)(4′-phenylaminoethoxyborylbenzyl)ether(778)

TG 16, x-Fold 0.85, SOC IC50 2 μM

Example 372(4-trifluoromethylphenylhydroxyborylphenyl)(4′-trifluoromethylphenylhydroxyborylbenzyl)ether(784)

TG −18, x-Fold 0.86, SOC IC50 1 μM

Example 373(4-trifluoromethylphenylaminoethoxyborylphenyl)(4′-trifluoromethylphenylaminoethoxyborylbenzyl)ether(785)

TG 1, x-Fold 0.84, SOC IC50 2 μM

Example 374 9,10-bis-(trifluoromethylphenylhydroxyboryl)anthracene (764)

TG 17, x-Fold 1.14

Example 375 9,10-bis-(trifluoromethylphenylaminoethoxyboryl)anthracene(787)

TG 44, x-Fold 1.05

Example 376 bis(3-(1-naphthylaminoethoxyboryl)benzyl)ether (788)

TG 75, x-Fold 0.93

Example 3774,5-di(phenylhydrixyboryl)-2,7-di-tert-butyl-9,9-dimethylxanthrene (763)

TG 70, x-Fold 0.75, SOC IC50 >20 μM

Example 3784,5-di(phenylaminoethoxyboryl)-2,7-di-tert-butyl-9,9-dimethylxanthrene(765)

TG 88, x-Fold 0.79

Example 379(4-(phenylhydroxyboryl)phenoxyethyl)(4-(phenylhydroxyboryl)benzyl)ether(818)

TG 92, x-Fold 0.74

Example 380(4-(phenylaminoethoxyboryl)phenoxyethyl)(4-(phenylaminoethoxyboryl)benzyl)ether(820)

TG 92, x-Fold 0.67

Example 381 6,6′-(phenylhydroxyboryl)-2,2′-dipyridyl (813)

TG 55, x-Fold 0.80

Example 382 6,6′-(phenylaminoethoxyboryl)-2,2′-dipyridyl (814)

TG 76, x-Fold 0.80

Example 383 bis(2,5-(phenylhydroxyboryl))furan (914)

TG 103, x-Fold 0.92

Example 384 bis(2,5-(phenylaminoethoxyboryl))furan (915)

TG 60, x-Fold 1.05

Example 385 bis(4,4′-(phenyl-N,N-dimethylaminoethoxyboryl)phenyl)ether(1007)

TG 116, x-Fold 0.78

Example 386 bis(4,4′-(phenyl-N-methylaminoethoxyboryl)phenyl)ether(1014)

TG 10, x-Fold 0.98, SOC IC50 0.5 μM

Example 387 2,8-di(phenylhydroxyboryl)dibenzothiophene (8012)

TG 96, x-Fold 0.73

2,8-Dibromodibenzothiophene (242 mg) was dissolved in ether (7 mL), andthe mixture was cooled to −78° C. Secondary butyllithium (2 mL) wasadded and the mixture was stirred for 1 hr. Further, isopropoxyborane(460 μL) was added and the mixture was stirred for 1 hr (SOLUTION A). Ina separate flask, bromobenzene (211 mg) was dissolved in ether (10 ml),secondary butyllithium (2 mL) was added and the mixture was stirred for1 hr (SOLUTION B). SOLUTION A and SOLUTION B were mixed, and the mixturewas gradually warmed to room temperature. The mixture was treated withhydrochloric acid the next morning to give the title compound (150 mg).

NMR (CDCl₃) 4.3 (s, 2H), 6.8-8.2 (m, 16H)

Example 388 bis(4,4′-(phenyl-glutamineboryl)phenyl)ether (7085)

TG 41, x-Fold 0.67, SOC IC50 0.5 μM

Example 3892,8-di(3-thiophenyl-2-pyrrolidinomethoxyboryl)dibenzothiophene (8019)

TG 81, x-Fold 0.83

Compound 8012 (Example 387) (25 mg) and 2-pyrrolidinemethanol (18 mg)were stirred in ethanol at room temperature for 5 hr to give the titlecompound (4.9 mg).

NMR (CDCl₃) 1.6-1.8 (m, 8H), 3.42-4 (m, 4H), 4.64 (m, 4H), 7.0-7.8 (m,12H)

Example 390 bis(4,4′-(phenyl-asparagineboryl)phenyl)ether (1023)

TG 56, x-Fold 0.59

Example 391(4-(phenyl-N-methylaminoethoxyboryl)phenyl)(4′-(hydroxymethylphenyl-N-methylaminoethoxyboryl)phenyl)ether(1028)

TG 15, x-Fold 0.32, SOC IC50 0.5 μM

Example 392(4-(phenyl-N,N-dimethylaminoethoxyboryl)phenyl)(4′-(hydroxymethylphenyl-N,N-dimethylaminoethoxyboryl)phenyl)ether(1030)

TG 83, x-Fold 0.91

Example 393 (4-(phenyl-glutamic acidboryl)phenyl)(4′-(hydroxymethylphenyl-glutamic acid boryl)phenyl)ether(1036)

TG 117, x-Fold 0.56

Example 394(4-(phenyl-glutamineboryl)phenyl)(4′-(hydroxymethylphenyl-glutamineboryl)phenyl)ether(1037)

TG 41, x-Fold 0.44, SOC IC50 1.5 μM

Example 395 bis(4,4′-(phenyl-N,N-dimethylaminoethoxyboryl)phenyl)ether(1007)

TG 116, x-Fold 0.86

Example 396 bis(4,4′-(phenyl-N-aminoethyl-aminoethoxyboryl)phenyl)ether(1040)

TG 3, x-Fold 0.58, SOC IC50 1.2 μM

Example 397(4-(phenyl-cysteineboryl)phenyl)(4′-(hydroxymethylphenyl-cysteineboryl)phenyl)ether(1038)

TG 70, x-Fold 0.59

Example 398 bis(4,4′-(phenoxyphenyl-aminoethoxyboryl)phenyl)ether (1042)

TG −17, x-Fold 0.88

Example 399 bis(4,4′-(phenyl-N-aminoethyl-aminoethoxyboryl)benzyl)ether(1084)

TG 53, x-Fold 0.96

Example 400 bis(4,4′-(phenyl-N-methylaminoethoxyboryl)benzyl)ether(2047)

TG 52, x-Fold 1.01

Example 401(4′-trifluoromethylphenyl-N,N-dimethylaminoethoxyboryl)-4-phenyl(4′-trifluoromethylphenyl-N,N-dimethylaminoethoxyborylbenzyl)ether(1139)

TG 121, x-Fold 0.95

Example 402(4′-trifluoromethylphenyl-N-methylaminoethoxyboryl)-4-phenyl(4′-trifluoromethylphenyl-N-methylaminoethoxyboryl-4-benzyl)ether(1140)

TG −12, x-Fold 0.57

Example 403 bis(3,3′-(phenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether(2022)

TG 67, x-Fold 1.14, SOC IC50 2 μM

Example 404 bis(3,3′-(phenyl-asparagineboryl)benzyl)ether (2023)

TG 105, x-Fold 1.07, SOC IC50 4 μM

Example 405 bis(3,3′-(phenyl-aminoethylthioboryl)benzyl)ether (3014)

TG −3, x-Fold 0.86, SOC IC50 0.5 μM

Example 406 2,8-di(3-thiophenylhydroxyboryl)dibenzothiophene (8013)

TG 61, x-Fold 0.85

2,8-Dibromodibenzothiophene (242 mg) was lithiated, and reacted withtriisopropoxyborane (499 mg) (SOLUTION A). Bromothiophene (326 mg) waslithiated (SOLUTION B). SOLUTION A and SOLUTION B were mixed at −78° C.,and the mixture was gradually warmed to room temperature to synthesizethe title compound (230 mg).

NMR (DMSO) 3.45 (m, 2H), 7.5-8.1 (m, 12H)

Example 407 bis(4,4′-(p-trifluoromethylphenyl-hydroxyboryl)benzyl)ether(2052)

TG 77, x-Fold 1.02

Example 408 2,8-di(phenylaminoethoxyboryl)dibenzothiophene (8014)

TG 108, x-Fold 0.92

Compound 8012 (Example 387) (30 mg) and 2-aminoethanol (7.4 mg) weresynthesized by stirring at room temperature for 5 hr to give the titlecompound (6.3 mg).

NMR (CDCl₃), 2.60 (m, 4H), 3.50 (m, 4H), 3.98 (m, 4H) 7.2-8.0 (m, 16H)

Example 409 bis(4,4′-(phenyl-lysineboryl)benzyl)ether (2051)

TG 29, x-Fold 0.86, SOC IC50 1.5 μM

Example 410 bis(4,4′-(p-methoxy-phenyl-hydroxyboryl)benzyl)ether (2072)

TG 130, x-Fold 0.90, SOC IC50 2 μM

Example 411 bis(4,4′-(3,4-difluorophenyl-hydroxyboryl)benzyl)ether(2073)

TG 138, x-Fold 0.90

Example 412 bis(4,4′-(p-methoxyphenyl-aminoethoxyboryl)benzyl)ether(2074)

TG 65, x-Fold 0.89, SOC IC50 2 μM

Example 413bis(4,4′-(p-methoxyphenyl-N-methylaminoethoxyboryl)benzyl)ether (2075)

TG 28, x-Fold 0.81, SOC IC50 0.8 μM

Example 414bis(4,4′-(p-methoxyphenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether(2076)

TG 128, x-Fold 0.90

Example 415 bis(4,4′-(p-methoxyphenyl-2,4-diaminobutyric acidboryl)benzyl)ether (2077)

TG 130, x-Fold 0.90

Example 416 bis(4,4′-(3,4-difluorophenyl-aminoethoxyboryl)benzyl)ether(2078)

TG 114, x-Fold 0.92

Example 417bis(4,4′-(3,4-difluorophenyl-N-methylaminoethoxyboryl)benzyl)ether(2079)

TG 91, x-Fold 1.01

Example 418bis(4,4′-(3,4-difluorophenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether(2080)

TG 45, x-Fold 1.02

Example 419bis(4,4′-(3,4-difluorophenyl-N-aminoethylaminoethoxyboryl)benzyl)ether(2081)

TG 140, x-Fold 0.90

Example 420bis(4,4′-(3-chloro-4-methylphenyl-aminoethoxyboryl)benzyl)ether (2056)

TG −3, x-Fold 0.81, SOC IC50 1.2 μM

Example 421bis(4,4′-(3-chloro-4-methylphenyl-N-methylaminoethoxyboryl)benzyl)ether(2057)

TG −1, x-Fold 1.03, SOC IC50 1.2 μM

Example 422bis(4,4′-(3-chloro-4-methylphenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether(2058)

TG 13, x-Fold 0.95, SOC IC50 1.2 μM

Example 423bis(4,4′-(3-chloro-4-methylphenyl-2-piperidylmethoxyboryl)benzyl)ether(2059)

TG 27, x-Fold 0.76, SOC IC50 1.2 μM

Example 424bis(4,4′-(p-trifluoromethylphenyl-N,N-dimethylaminoethoxyboryl)benzyl)ether(2063)

TG 22, x-Fold 1.03, SOC IC50 1.2 μM

Example 425bis(4,4′-(p-trifluoromethylphenyl-asparagineboryl)benzyl)ether (2064)

TG 130, x-Fold 0.9, SOC IC50 0.5 μM

Bis(4,4′-(p-trifluoromethylphenyl-hydroxyboryl)benzyl)ether (85 mg) andasparagine (48 mg) were reacted in ethanol (0.7 mL) to give the titlecompound (8 mg).

Example 426bis(4,4′-(p-trifluoromethylphenyl-aminoethoxyboryl)benzyl)ether (2068)

TG 19, x-Fold 0.93, SOC IC50 1.2 μM

Example 427 (4-phenyl-N-methylaminoethoxyborylphenyl)(4′-phenyl-N-methylaminoethoxyborylbenzyl)ether (2093)

TG 20, x-Fold 0.73, SOC IC50 0.8 μM

Example 428 (4-phenyl-N,N-dimethylaminoethoxyborylphenyl)(4′-phenyl-N,N-dimethylaminoethoxyborylbenzyl)ether (2094)

TG 53, x-Fold 0.82, SOC IC50 1.5 μM

Example 429(4-phenyl-2-pyridylmethoxyborylphenyl)(4′-phenyl-2-pyridylmethoxyborylbenzyl)ether(2095)

TG 102, x-Fold 0.81, SOC IC50 0.7 μM

Example 430 4-(phenyl-p-methoxyphenyl-2-pyridylmethoxyboryl)-phenyl4′-(phenyl-p-methoxyphenyl-2-pyridylmethoxyboryl)benzylether (2096)

TG 106, x-Fold 1.03

Example 431 bis(4,4′-(phenyl-3-piperidyloxyboryl)phenyl)ether (2052)

TG 118, x-Fold 1.02

Example 432 bis(4,4′-(phenyl-2-pyridylmethoxyboryl)phenyl)ether (2111)

TG 60, x-Fold 0.71, SOC IC50 0.3 μM

Example 433 bis(4,4′-(phenyl-aminoethylthioboryl)phenyl)ether (2112)

TG −5, x-Fold 0.71, SOC IC50 0.5 μM

Example 434 bis(4,4′-(phenyl-2-amino-1-phenylethoxyboryl)phenyl)ether(2113)

TG 43, x-Fold 0.60, SOC IC50 0.4 μM

Example 435 bis(4,4′-(phenyl-ornithineboryl)phenyl)ether (2117)

TG 26, x-Fold 0.84, SOC IC50 2 μM

Example 436 bis(4,4′-(phenyl-2,3-diaminopropionic acidboryl)phenyl)ether (2115)

TG 104, x-Fold 0.85

Example 437 bis(4,4′-(phenyl-lysineboryl)phenyl)ether (2116)

TG 119, x-Fold 0.85

Example 438 bis(4,4′-(phenyl-2-pyrrolidinemethoxyboryl)phenyl)ether(2118)

TG 29, x-Fold 0.67, SOC IC50 2 μM

Example 439 bis(4,4′-(naphthylhydroxyboryl)phenyl)ether (2119)

TG 33, x-Fold 0.54

Example 440 bis(4,4′-(tolylhydroxyboryl)phenyl)ether (2120)

TG 63, x-Fold 0.69

Example 441 bis(4,4′-(naphthyl-aminoethoxyboryl)phenyl)ether (2121)

TG −1, x-Fold 0.58

Example 442 bis(4,4′-(naphthyldimethylaminoethoxyboryl)phenyl)ether(2122)

TG 102, x-Fold 0.58

Example 443 bis(4,4′-(naphthyl-2-pyridylmethoxyboryl)phenyl)ether (2123)

TG 84, x-Fold 0.63, SOC IC50 3 μM

Example 444 bis(4,4′-(naphthylglutamineboryl)phenyl)ether (2124)

TG 20, x-Fold 0.65, SOC IC50 1.4 μM

Example 445 bis(4,4′-(naphthyl 2,4-diaminopropionic acidboryl)phenyl)ether (2125)

TG 108, x-Fold 0.49

Example 446 bis(4,4′-(tolyldimethylaminoethoxyboryl)phenyl)ether (2127)

TG 73, x-Fold 0.85

Example 447 bis(4,4′-(tolylpiperadylethoxyboryl)phenyl)ether (2128)

TG 97, x-Fold 0.49

Example 448 di(3-chloro-4-methyl)phenyl(methionate-O,N)borane (4103)

TG 112, x-Fold 0.95

Di(3-chloro-4-methylphenyl)borinic acid (45.8 mg) and asparagine (19 mg)were reacted in ethanol (1 mL) at 90° C. for 1 hr to give the titlecompound (24 mg).

Example 449 bis(4,4′-(tolylasparagineboryl)benzyl)ether (2129)

TG 92, x-Fold 0.89

Example 450 bis(4,4′-(tolyllysineboryl)phenyl)ether (2130)

TG 53, x-Fold 0.49

Example 451 bis(4,4′-(phenyl-aminoethylthioboryl)benzyl)ether (2135)

TG 6, x-Fold 0.91, SOC IC50 1.4 μM

Example 452 bis(4,4′-(phenyl-2-pyrrolidinemethoxyboryl)benzyl)ether(2136)

TG 29, x-Fold 0.96, SOC IC50 0.5 μM

Example 453 bis(4,4′-(phenyl-2,4-diaminobutyrate boryl)benzyl)ether(2137)

TG 113, x-Fold 1.04

Example 454 bis(4,4′-(phenyl-butylaminoethoxyboryl)benzyl)ether (2144)

TG 15, x-Fold 0.97, SOC IC50 0.5 μM

Example 455 bis(4,4′-(phenyl-phenylaminoethoxyboryl)benzyl)ether (2145)

TG 23, x-Fold 1.04, SOC IC50 0.5 μM

Example 456 bis(4,4′-(phenyl-benzylaminoethoxyboryl)benzyl)ether (2146)

TG 29, x-Fold 0.87, SOC IC50 0.5 μM

Example 457bis(4,4′-(phenyl-N-methylpiperidine-methoxyboryl)benzyl)ether (3002)

TG 30, x-Fold 1.10, SOC IC50 0.6 μM

Example 458 bis(4,4′-(phenyl-1-methyl-2-aminoethoxyboryl)benzyl)ether(3004)

TG 31, x-Fold 1.10, SOC IC50 0.5 μM

Example 459 bis(4,4′-(phenyl-1-piperidylethoxyboryl)benzyl)ether (3005)

TG 80, x-Fold 1.03

Example 460 bis(3,3′-(phenyl-2-pyrrolidinomethoxyboryl)benzyl)ether(3015)

TG 26, x-Fold 0.95, SOC IC50 0.4 μM

Example 461 poly(1,4-phenylene 2-pyridylmethoxyborane) (6078)

TG 30, x-Fold 0.85

Example 462 bis(3,3′-(phenyl-2-phenyl-2-aminoethoxyboryl)benzyl)ether(3018)

TG 31, x-Fold 0.92, SOC IC50 0.3 μM

Example 463 bis(3,3′-(phenyl-2-piperidylmethoxyboryl)benzyl)ether (3020)

TG 24, x-Fold 0.92, SOC IC50 0.3 μM

Example 464 bis(3,3′-(phenyl-dimethylaminoethoxyboryl)benzyl)ether(3021)

TG 41, x-Fold 0.76, SOC IC50 0.8

Example 465 bis(3,3′-(phenyl-1-methyl-2-aminoethoxyboryl)benzyl)ether(3022)

TG 18, x-Fold 1.06, SOC IC50 0.2 μM

Example 466 bis(3,3′-(phenyl-1-piperidylethoxyboryl)benzyl)ether (3023)

TG 71, x-Fold 1.04

Example 467 bis(3,3′-(phenyl-2-pyridylmethoxyboryl)benzyl)ether (3024)

TG 60, x-Fold 0.98, SOC IC50 0.25 μM

Example 468 bis(3,3′-(phenyl-2-amino-1-phenylethoxyboryl)benzyl)ether(3025)

TG 35, x-Fold 0.98, SOC IC50 0.3 μM

Example 469 bis(3,3′-(phenyl-N-methylaminoethoxyboryl)benzyl)ether(3026)

TG 15, x-Fold 0.94, SOC IC50 0.25 μM

Example 470bis(3,3′-(phenyl-N-aminoethyl-1-methyl-2-aminoethoxyboryl)benzyl)ether(3027)

TG 19, x-Fold 1.02, SOC IC50 0.3 μM

Example 471 bis(3,3′-(phenyl-glutamineboryl)benzyl)ether (3028)

TG 52, x-Fold 1.04, SOC IC50 0.6 μM

Example 472 bis(3,3′-(phenyl-2,4-diaminobutyric acid boryl)benzyl)ether(3029)

TG 47, x-Fold 0.95, SOC IC50 1 μM

Example 473 bis(3,3′-(phenyl-N-butylaminoethoxyboryl)benzyl)ether (3030)

TG-4, x-Fold 0.96, SOC IC50 0.5 μM

Example 474 bis(3,3′-(phenyl-asparagineboryl)benzyl)ether (3031)

TG 145, x-Fold 1.04, SOC IC50 0.5 μM

Example 475 bis(3,3′-(phenyl-lysineboryl)benzyl)ether (3032)

TG 21, x-Fold 1.01, SOC IC50 0.6 μM

Example 476 bis(3,3′-(phenyl-ornithineboryl)benzyl)ether (3033)

TG 103, x-Fold 0.95, SOC IC50 1.5 μM

Example 477 bis(4,4′-(phenyl-2-methyl-8-quinolinooxyboryl)phenyl)ether(3037)

TG 97, x-Fold 1.02

Example 478 poly(diphenyletherhydroxyborane) (7142)

TG 121

4,4′-Dibromodiphenylether (28 mg) was lithiated using isobutyllithiumand reacted with triisopropoxyborane to give the title compound (150mg).

NMR (CDCl₃) 3.45 (br, 1H), 6.7-8.0 (m, 8H)

Example 479 bis(4,4′-(phenyl-2-benzyl-2-amino-ethoxyboryl)benzyl)ether(3076)

TG 54, x-Fold 1.00, SOC IC50 1.5 μM

Example 480 bis(4,4′-(phenyl-2-benzyl-2-amino-ethoxyboryl)phenyl)ether(3077)

TG 59, x-Fold 0.66, SOC IC50 1.5 μM

Example 481 bis(3,3′-(phenyl-2-benzyl-2-amino-ethoxyboryl)benzyl)ether(3085)

TG 48, x-Fold 0.80, SOC IC50 1.5 μM

Example 482 2,8-di(phenylglutamine-O,N borane)dibenzothiophene (8015)

TG 114, x-Fold 1.08

Compound 8012 (Example 387) (40 mg) and glutamine (31 mg) were reactedat 80° C. to give the title compound (15 mg).

NMR (DMSO) 2.2 (m, 2H), 2.5 (m, 4H), 3.3 (m, 10H), 7.0-7.8 (m, 16H)

Example 483 2,8-di(phenyl 2-pyrrolidinomethoxyboryl)dibenzothiophene(8016)

TG 107, x-Fold 0.73

The title compound (37 mg) was obtained from compound 8013 (Example 406)(30 mg) and 2-pyrrolidinemethanol (16 mg).

NMR (DMSO) 1.05 (m, 4H), 1.7 (m, 4H), 3.3-3.5 (m, 4H), 7.7-8.0 (m, 16H)

Example 484 2,8-di(phenylarginine-O,N borane)dibenzothiophene (8017)

TG 82, x-Fold 0.78

The title compound (30 mg) was obtained from compound 8012 (Example 387)(24 mg) and arginine (32 mg).

NMR (DMSO) 1.06 (m, 2H), 2.60 (m, 4H), 3.3 (m, 6H), 7.1-7.8 (m, 16H)

Example 485 2,8-di(3-thiophenylaminoethoxyboryl)dibenzothiophene (8018)

TG 76, x-Fold 0.98

The title compound (6.4 mg) was obtained from compound 8013 (Example406) (42 mg) and ethanolamine (14 mg).

NMR (CDCl₃) 2.41 (4H), 2.65 (m, 4H), 3.65 (m, 4H), 7.0-7.9 (m, 12H)

Example 486 bis(2,2′-(phenylhydroxyboryl)benzyl)ether (161OH)

TG 52, x-Fold 1.04, SOC IC50 0.5 μM

Example 487 2-aminoethyl diphenylborinate (2APB)

TG 90, x-Fold 0.64, SOC IC50 3 μM

Example 488 diphenylborinic acid (3036)

TG 108, x-Fold 1.01, SOC IC50 4 μM

Example 489 poly(4,4′-biphenylene aminoethylthioborane) (1130)

TG 118, x-Fold 0.80

Example 490 poly(4-phenylborinic acid) (502)

TG 111, x-Fold 0.94

Example 491 poly(dimethylaminoethoxyphenyleneborane) (1078)

TG 106, x-Fold 0.84

Example 492 1,3,5-tri(phenyl 2-aminoethoxyboryl)benzene (564)

Example 493 dibutyl(phenylalanine-O,N)borane (929)

TG 106, x-Fold 1.03

TABLE 1 Example SOC IC50 Compound No. No. TG x-Fold (μM) 6014 1 28 0.957111 2 28 0.82 0.2 536 3 −20 0.49 0.5 1130 4 109 0.80 5 1022 5 −4 0.600.15 7132 6 23 1.01 0.2 1620H 7 14 1.03 0.2 162AE 8 24 1.1 0.2 6077 9 120.87 0.5 6076 10 7 0.92 0.5 6047 11 36 0.99 6050 12 91 1.04 1122 13 1001.11 1132 14 85 1.03 1133 15 91 0.90 1134 16 86 0.95 503 17 111 0.651042D 18 −17 0.84 1.5 1042E 19 47 0.86 1056 20 54 0.63 4 1120 21 1110.72 1121 22 30 0.62 1107 23 114 0.62 1116 24 96 0.78 1117 25 12 0.691109 26 116 0.78 1108-3 27 45 0.86 5 1114 28 94 0.72 1115 29 52 0.831141c 30 107 1.02 1146 31 127 0.95 3115 32 12 1.02 1 6048 33 51 0.926051 34 39 1.01 6053 35 14 0.98 1068 36 6 0.65 3 1074 37 −22 0.73 107738 79 0.71 1060 39 99 1.04 1062 40 26 0.52 1063 41 54 0.63 2 1064 42 80.53 2 1065 43 13 0.73 3 1066 44 12 0.54 4 1097 45 99 0.52 1102 46 930.50 1103 47 106 0.58 1104 48 102 0.59 2102 49 89 0.96 1105 50 112 0.591106 51 13 0.43 1069 52 73 0.69 1075 53 113 0.74 1080 54 112 0.67 108155 151 0.71 1082 56 74 0.71 1125 57 5.98 0.67 4 1124 58 45 0.62 1126 59107 0.72 1127 60 24 0.73 1123 61 100 0.99 1135 62 94 0.95 1136 63 631.04 1137 64 11 0.95 1142 65 115 1.02 7 1144 66 120 1.18 >20 1145 67 1220.87 6060 68 119 1.04 5034 69 76 1.02 5141 70 13 0.73 0.3 5142 71 510.97 1 5143 72 41 1.02 0.5 5144 73 35 0.85 1.2 5145 74 41 0.95 1 6001 7597 0.88 6004 76 117 0.78 6006 77 98 0.91 6007 78 104 1.02 6008 79 970.88 6009 80 93 0.90 6010 81 97 0.92 6011 82 103 0.95 6012 83 101 0.926013 84 91 0.92 504 85 128 0.79 6015 86 103 0.99 6016 87 91 1.02 6017 8882 0.83 6018 89 80 0.94 6019 90 93 0.81 6020 91 107 0.99 6021 92 1061.00 6023 93 117 0.93 6024 94 114 0.95 6025 95 114 0.88 6026 96 124 0.866027 97 122 0.72 6029 98 111 0.95 6030 99 109 0.73 6032 100 119 0.976033 101 122 1.02 5009 102 72 1.10 6034 103 114 0.89 6037 104 94 1.166038 105 92 1.05 6039 106 23 0.92 6040 107 111 0.98 6041 108 111 1.006042 109 108 1.02 >10 6043 110 115 1.02 >10 6044 111 121 1.02 6046 112123 0.99 6059 113 112 0.99 6059-9 114 120 0.99 2 385 115 101 1.07 419116 108 1.02 434 117 108 0.06 1.5 544 118 93 0.97 2 554 119 101 0.84 >20805 120 88 1.08 583 121 121 0.94 880 122 93 0.98 7 870 123 98 0.84 1 656124 90 0.96 595 125 113 10 601 126 81 1.04 592 127 109 0.70 573 128 1430.93 1016 129 101 0.78 563 130 116 0.85 163AE 131 16 1.1 0.3 567 132 880.95 566 133 106 1.00 558 134 94 0.92 602 135 99 1.03 871 136 96 0.981630H 137 14 0.99 0.3 607 138 96 0.99 611 139 122 0.88 548 140 −72 0.85620 141 97 0.92 621 142 88 0.24 618 143 118 0.90 612 144 99 0.87 6005145 97 0.91 803 146 91 1.02 554 147 101 0.87 20 557 148 68 1.00 607 14996 0.99 4122 150 2 0 1031 151 33 0.87 1073 152 54 1.07 1079 153 65 0.791089 154 105 0.96- 427 155 100 1.02 7138 156 91 1.08 1116 157 96 0.731117 158 12 0.69 926 159 102 0.96 7139 160 88 1.02 1098 161 6 0.99 1099162 −2 0.85 347 163 109 1.00 376 164 94 0.67 1143 165 120 0.99 372 16674 0.70 2006 167 21 0.71 2007 168 35 0.72 1016 169 101 0.78 907 170 960.96 370 171 98 0.71 2024 172 69 1.22 2026 173 122 1.06 2031-4 174 1030.99 2033 175 5 0.89 2035 176 47 1.06 2036 177 28 1.00 2039 178 142 0.892044 179 127 0.99 4124 180 35 0.98 424 181 54 0.69 4105 182 137 1.01 925183 91 1.02 2049 184 94 0.95 2064 185 130 0.94 >20 601 186 81 0.98 2086187 106 0.97 428 188 91 0.98 2088 189 119 0.94 2089 190 99 1.05 2090 19185 1.04 2091 192 102 0.95 899 193 92 1.03 901 194 106 1.03 2108 195 1150.77 2109 196 117 0.90 3001 197 99 1.02 3003 198 28 0.8 3017 199 3 0.90442 200 100 0.92 431 201 99 0.57 3041 202 91 0.94 3044 203 97 0.97 3045204 61 0.79 3087 205 47 0.80 3107 206 34 1.14 3108 207 83 0.91 3109 208−7 0.67 3111 209 1 0.98 3112 210 27 0.98 2 3113 211 86 0.99 1 3073 212115 0.75 3075 213 117 1.00 3114 214 −7 0.90 2 3116 215 69 1.03 2 4139216 17 1.03 0.6 4111 217 118 0.94 4118 218 90 0.97 4119 219 91 0.88 4121220 26 0.50 0.5 4123 221 73 0.94 8003 222 122 0.86 8006 223 116 1.024127 224 112 0.89 4128 225 109 1.03 0.5 4129 226 97 0.94 4130 227 1100.99 4131 228 99 0.98 4132 229 40 1.09 0.5 4138 230 108 1.03 4140 231 941.01 4141 232 108 1.10 4142 233 112 1.12 4143 234 98 1.07 0.5 4144 23580 1.03 4145 236 87 1.10 4146 237 88 1.15 4147 238 87 1.07 356 239 1260.94 7117 240 25 0.99 0.08 244 241 67 1.10 371 242 98 1.17 436 243 1060.73 372 244 74 0.76 1 921 245 94 0.91 376 246 94 0.67 422 247 99 0.910.7 421 248 103 0.87 7118 249 25 0.74 0.3 1007 250 125 0.86 488 251 1210.83 542 252 93 0.95 0.5 283 253 92 1.11 827 254 101 0.95 828 255 1130.94 0.5 829 256 112 0.67 1.5 830 257 103 0.98 833 258 110 5 841 259 670.97 2.5 836 260 106 0.89 837 261 109 0.89 838 262 115 0.97 2045 263 1460.89 3 842 264 109 1.00 5 851 265 112 0.94 847 266 84 0.87 3 848 267 820.60 3 852 268 103 0.96 5 879 269 95 1.01 3 855 270 111 0.54 0.7 906 271109 1.07 0.5 2043 272 83 0.09 0.3 1024 273 83 0.56 0.25 1023 274 56 0.590.3 1036 275 117 0.67 0.3 854 276 105 0.8 843 277 105 0.98 0.3 7119 2782 1.08 0.3 894 279 103 0.98 897 280 98 0.88 4123 281 77 0.94 4103 282112 0.95 0.3 4125 283 12 0.83 0.9 5003 284 89 1.03 5004 285 51 0.99 25012 286 104 0.93 5013 287 146 1.00 5014 288 106 1.02 5015 289 94 1.080.3 5018 290 113 1.05 5019 291 50 1.02 0.5 5020 292 146 1.00 1 5021 293116 0.91 4106 294 114 0.96 2 4107 295 107 0.92 0.8 795 296 97 0.74 806297 89 0.69 810 298 101 1.01 8007 299 118 1.13 1085 300 95 0.80 5 1083301 108 0.84 6062 302 103 0.94 6082 303 103 0.91 8020 304 47 0.90 6095305 94 0.98 6096 306 90 0.98 7021 307 54 1.06 0.5 7020 308 27 1.05 0.57047 309 109 0.93 7051 310 114 1.02 7052 311 111 1.00 7053 312 98 1.007056 313 107 0.98 7057 314 104 0.93 7058 315 102 0.92 7059 316 72 1.117063 317 107 0.99 7064 318 81 1.02 7065 319 108 1.04 1128 320 100 0.78 51129 321 116 0.78 612 322 98 0.32 0.2 502 323 111 0.82 7126 324 0.762054 325 92 0.99 4 8009 326 103 1.09 8010 327 14 1.07 2072 328 100 1.04672 329 81 0.2 655 330 89 0.90 682 331 101 0.98 1 674 332 21 0.98 0.2701 333 107 1.09 687 334 21 1.02 0.3 686 335 91 1.02 688 336 101 1.02689 337 102 0.98 693 338 110 0.83 696 339 115 0.91 700 340 63 1.01 701341 107 1.04 702 342 114 1.02 704 343 55 1.02 705 344 91 0.93 706 345 950.92 707 346 101 0.81 708 347 104 0.90 710 349 104 0.80 717 350 105 0.92711 351 103 1.00 718 352 97 1.02 712 353 115 0.85 719 354 113 1.09 731355 91 1.09 735 356 51 1.06 736 357 89 1.03 739 358 112 0.91 744 359 1390.96 745 360 88 1.05 709 361 100 0.88 >20 729 362 108 1.08 752 363 970.92 754 364 44 0.82 753 365 118 0.91 8011 366 108 0.93 513 367 113 0.736055 368 52 1.03 7133 369 105 1.10 775 370 39 0.76 2 778 371 16 0.85 2784 372 −18 0.86 1 785 373 1 0.84 2 764 374 17 1.14 787 375 44 1.05 788376 75 0.93 763 377 70 0.75 >20 765 378 88 0.79 818 379 92 0.74 820 38092 0.67 813 381 55 0.80 814 382 76 0.80 914 383 103 0.92 915 384 60 1.051007 385 116 0.78 1014 386 10 0.98 0.5 8012 387 96 0.73 7085 388 41 0.670.5 8019 389 81 0.83 1023 390 56 0.59 1028 391 15 0.32 0.5 1030 392 830.91 1036 393 117 0.56 1037 394 41 0.44 1.5 1007 395 116 0.86 1040 396 30.58 1.2 1038 397 70 0.59 1042 398 −17 0.88 1084 399 53 0.96 2047 400 521.01 1139 401 121 0.95 1140 402 −12 0.57 2022 403 67 1.14 2 2023 404 1051.07 4 3014 405 −3 0.86 0.5 8013 406 61 0.85 2052 407 77 1.02 8014 408108 0.92 2051 409 29 0.86 1.5 2072 410 130 0.90 2 2073 411 138 0.90 2074412 65 0.89 2 2075 413 28 0.81 0.8 2076 414 128 0.90 2077 415 130 0.902078 416 114 0.92 2079 417 91 1.01 2080 418 45 1.02 2081 419 140 0.902056 420 −3 0.81 1.2 2057 421 −1 1.03 1.2 2058 422 13 0.95 1.2 2059 42327 0.76 1.2 2063 424 22 1.03 1.2 2064 425 130 0.9 0.5 2068 426 19 0.931.2 2093 427 20 0.73 0.8 2094 428 53 0.82 1.5 2095 429 102 0.81 0.7 2096430 106 1.03 2052 431 118 1.02 2111 432 60 0.71 0.3 2112 433 −5 0.71 0.52113 434 43 0.60 0.4 2117 435 26 0.84 2 2115 436 104 0.85 2116 437 1190.85 2118 438 29 0.67 2 2119 439 33 0.54 2120 440 63 0.69 2121 441 −10.58 2122 442 102 0.58 2123 443 84 0.63 3 2124 444 20 0.65 1.4 2125 445108 0.49 2127 446 73 0.85 2128 447 97 0.49 4103 448 112 0.95 2129 449 920.89 2130 450 53 0.49 2135 451 6 0.91 1.4 2136 452 29 0.96 0.5 2137 453113 1.04 2144 454 15 0.97 0.5 2145 455 23 1.04 0.5 2146 456 29 0.87 0.53002 457 30 1.10 0.6 3004 458 31 1.10 0.5 3005 459 80 1.03 3015 460 260.95 0.4 6078 461 30 0.85 3018 462 31 0.92 0.3 3020 463 24 0.92 0.3 3021464 41 0.76 0.8 3022 465 18 1.06 0.2 3023 466 71 1.04 3024 467 60 0.980.25 3025 468 35 0.98 0.3 3026 469 15 0.94 0.25 3027 470 19 1.02 0.33028 471 52 1.04 0.6 3029 472 47 0.95 1 3030 473 −4 0.96 0.5 3031 474145 1.04 0.5 3032 475 21 1.01 0.6 3033 476 103 0.95 1.5 3037 477 97 1.027142 478 121 3076 479 54 1.00 1.5 3077 480 59 0.66 1.5 3085 481 48 0.801.5 8015 482 114 1.08 8016 483 107 0.73 8017 484 82 0.78 8018 485 760.98 1610H 486 52 1.04 0.5 2APB 487 90 0.64 3 3036 488 108 1.01 4 1130489 118 0.80 502 490 111 0.94 1078 491 106 0.84 564 492 929 493 106 1.03

Experimental Example 4

The effects of 162AE (bis(3,3′-(phenylaminoethoxyboryl)benzyl)etherdescribed in Example 8) and 163AE(bis(4,4′-(phenylaminoethoxyboryl)benzyl)ether described in Example 131)for I_(CRAC), whose molecular entity as one of SOCE has been clarified,were investigated using an electrophysiological method. STIM1 and Orail(CRACM1) were forcibly expressed in HEK293 cells, and whole cell recordswere taken by the Patch clamp technique. BAPTA (20 mM), which is acalcium chelator, and IP₃ (20 μM) that depletes intracellular calciumstore were added to a recording electrode internal solution (120 mMCs-glutamate, 10 mM HEPES, 3 mM MgCl₂), 10 mM calcium was added to anextracellular solution to facilitate observation of calcium electriccurrent, and a ramp command from −150 mV to +150 mV was input at 0.5 Hzto obtain a current-voltage curve. For quantification of SOCE, the sizeof the inward current at −80 mV was used as an index. After the start ofthe whole cell recording, time was taken to sufficiently activate SOCE(I_(CRAC)), and compounds 162AE and 163AE as inhibitors wereadministered to the cells. As a result of the experiment, theseinhibitors highly strongly inhibited SOCE (I_(CRAC)) and the IC50thereof was 0.086 μM, 0.17 μM (for 162AE, 163AE, respectively), thusexhibiting a strong inhibitory effect. Moreover, since SOCE (I_(CRAC))reconstituted by STIM1 and Orail (CRACM1) is indispensible for theimmune response of T cells, it is considered possible to suppress excessimmune response that occurs in autoimmune diseases, by utilizing theinhibitor, and treat the disease or mitigate the symptoms.

INDUSTRIAL APPLICABILITY

According to the present invention, a drug for the prophylaxis and/ortreatment of a disease based on abnormal protein cross-linking reaction,such as Alzheimer's disease, Parkinson's disease, Celiac disease,cataract, mad cow disease, congenital lamellar ichthyosis, congenitalhemostatic disorder and the like can be provided.

This application is based on a patent application No. 2008-207315 filedin Japan (filing date: Aug. 11, 2008), the contents of which areincorporated in full herein by this reference.

The invention claimed is:
 1. A compound of formula (8′)R₃′—B(ZR₁′)—X′—B(ZR₂′)—R₄′  (8′) wherein B is a boron atom, Z is O, R₁′and R₂′ are H, —(CH₂)_(m)—NH₂, —CH₂R₁₂′ wherein R_(12′) is pyrrolidinyl,—COCH(NH₂)—(CH₂)_(m)NHCONH₂, or —COCH(NH₂)—(CH₂)_(m)—CONH₂, and m is aninteger of 1 to 5, R₃′ and R₄′ are phenyl or thienyl, and X′ is a2,8-dibenzothiophenyl group, or a pharmaceutically acceptable saltthereof.
 2. The compound according to claim 1, which is any of

or a pharmaceutically acceptable salt thereof.
 3. The compound accordingto claim 1 of the formula

or a pharmaceutically acceptable salt thereof.
 4. A proteincross-linking inhibitor comprising the compound according to claim 1, ora pharmaceutically acceptable salt thereof.
 5. The inhibitor accordingto claim 4, wherein the inhibition is polyglutamine aggregationinhibition.
 6. A therapeutic drug for a disease caused by cross-linkingof protein, comprising the compound according to claim 1, or apharmaceutically acceptable salt thereof, wherein the disease isselected from Alzheimer's disease, Parkinson's disease, and mad cowdisease.
 7. A polyglutamine aggregation inhibitor comprising thecompound according to claim 1, or a pharmaceutically acceptable saltthereof.
 8. A therapeutic drug for a disease caused by polyglutamineaggregation, comprising the compound according to claim 1, or apharmaceutically acceptable salt thereof, wherein the disease isHuntington's disease.